What should I do if the genetic test shows no mutation? Do I need to retest?<\/h4><\/div><\/div><\/div><\/div><\/div><\/div><\/div>\u6700\u8fd1\u6709\u4e0d\u5c11\u521a\u786e\u8bca\u7684\u975e\u5c0f\u7ec6\u80de\u80ba\u764c\u75c5\u53cb\u63d0\u51fa\u4e00\u4e2a\u5171\u540c\u7684\u7591\u95ee\u2014\u2014\u201c\u7b2c\u4e00\u6b21\u57fa\u56e0\u68c0\u6d4b\u6ca1\u6709\u7a81\u53d8\u600e\u4e48\u529e\uff0c\u9700\u4e0d\u9700\u8981\u91cd\u65b0\u68c0\u6d4b\u201d\uff0c\u5173\u4e8e\u8fd9\u4e2a\u95ee\u9898\uff0c\u6211\u4ece\u57fa\u56e0\u68c0\u6d4b\u672c\u804c\u5de5\u4f5c\u7684\u89d2\u5ea6\u63d0\u51fa\u4ee5\u4e0b\u601d\u8def\u4f9b\u5404\u4f4d\u501f\u9274\u3002<\/p>\n
\u5f71\u54cd\u57fa\u56e0\u68c0\u6d4b\u7ed3\u679c\u7684\u56e0\u7d20\u5927\u81f4\u53ef\u5305\u62ec\u764c\u79cd\u672c\u8eab\u3001\u6837\u672c\u7c7b\u578b\u4ee5\u53ca\u68c0\u6d4b\u6280\u672f\u7b49\uff0c\u6211\u4eec\u4e0d\u8981\u53ea\u7740\u773c\u4e8e\u68c0\u6d4b\u7ed3\u679c\u7684\u6709\u65e0\uff0c\u8981\u5b66\u4f1a\u4ece\u6839\u672c\u4e0a\u53bb\u770b\u5f85\u3002<\/p>\n<\/div>
\u5f71\u54cd\u56e0\u7d20\u4e00\uff1a\u764c\u79cd<\/span><\/div><\/h4><\/div>\u6709\u53e5\u4fd7\u8bed\u8bf4\u201c\u9f99\u751f\u9f99\uff0c\u51e4\u751f\u51e4\u201d\uff0c\u5bd3\u610f\u662f\u4e0d\u540c\u4e8b\u7269\u7684\u7279\u70b9\u6709\u6240\u4e0d\u540c\uff0c\u4e0d\u53ef\u4e00\u6982\u800c\u8bba\uff0c\u80bf\u7624\u6cbb\u7597\u4e5f\u662f\u5982\u6b64\uff0c\u73b0\u9636\u6bb5\u5e76\u975e\u6240\u6709\u764c\u79cd\u90fd\u6709\u53ef\u53ca\u7684\u9776\u5411\u836f\uff0c\u800c\u6709\u5bf9\u5e94\u9776\u5411\u836f\u7684\u764c\u79cd\u7684\u9776\u5411\u57fa\u56e0\u548c\u7a81\u53d8\u6982\u7387\u4e5f\u6709\u6240\u4e0d\u540c\u3002<\/p>\n
\u4f8b\u5982\u4e73\u817a\u764c\u7684\u4e3b\u8981\u9776\u5411\u76f8\u5173\u57fa\u56e0\u4e3aHER2\u3001PIK3CA\u3001BRCA1\/2\uff0c\u9ed1\u8272\u7d20\u7624\u4e3aBRAF\uff0c\u975e\u5c0f\u7ec6\u80de\u80ba\u764c\u5219\u4e3aEGFR\u3001ALK\u3001ROS1\u3001RET\u3001MET\u3001HER2\u3001BRAF\u3001KRAS\u3001NTRK\u7b49\uff0c\u5176\u4e2dBRAF\u57fa\u56e0\u5728\u9ed1\u8272\u7d20\u7624\u4e2d\u7a81\u53d8\u6982\u7387\u8fbe\u523025%\u5de6\u53f3\uff0c\u5728\u975e\u5c0f\u7ec6\u80de\u80ba\u764c\u4e2d\u4ec5\u4e3a1-5%\u5de6\u53f3\u3002<\/p>\n
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\u5bf9\u4e8e\u57fa\u56e0\u68c0\u6d4b\uff0c\u5404\u4f4d\u5148\u4e0d\u8981\u7ea0\u7ed3\u6709\u65e0\u7a81\u53d8\uff0c\u662f\u5426\u9700\u8981\u4e8c\u6b21\u68c0\u6d4b\uff0c\u66f4\u91cd\u8981\u7684\u662f\u5b66\u4f1a\u770b\u75c5\u7406\u62a5\u544a\uff0c\u4e86\u89e3\u75c5\u7406\u8bca\u65ad\uff0c\u660e\u786e\u81ea\u8eab\u662f\u4f55\u764c\u79cd\uff0c\u6709\u65e0\u5bf9\u5e94\u9776\u5411\u836f\uff0c\u9776\u5411\u57fa\u56e0\u7a81\u53d8\u5927\u81f4\u6982\u7387\u6709\u591a\u5c11\uff0c\u82e5\u662f\u65e0\u9776\u5411\u836f\u6216\u8005\u9776\u5411\u57fa\u56e0\u7a81\u53d8\u6982\u7387\u4f4e\u7684\u764c\u79cd\uff0c\u90a3\u5219\u4e0d\u5fc5\u8fc7\u4e8e\u6267\u7740\u57fa\u56e0\u68c0\u6d4b\u3002<\/p>\n<\/div>
![](\"https:\/\/www.funcbio.cn\/wp-content\/uploads\/2023\/11\/7-1.jpg\" "\\"7-1\\"")
<\/span><\/div>\u5f71\u54cd\u56e0\u7d20\u4e8c\uff1a\u68c0\u6d4b\u6837\u672c<\/div><\/h4><\/div>\u660e\u786e\u764c\u79cd\uff0c\u4e86\u89e3\u662f\u5426\u6709\u5bf9\u5e94\u9776\u5411\u836f\uff0c\u77e5\u6653\u57fa\u56e0\u68c0\u6d4b\u53c2\u8003\u4ef7\u503c\u53ea\u662f\u65b9\u5411\uff0c\u65b9\u5411\u5bf9\u4e86\u8fd8\u8981\u770b\u8d70\u7684\u662f\u5426\u8e0f\u5b9e\uff0c\u57fa\u7840\u662f\u5426\u7262\u9760\uff0c\u800c\u57fa\u56e0\u68c0\u6d4b\u7684\u57fa\u7840\u5c31\u662f\u6837\u672c\uff0c\u4e0d\u540c\u7684\u6837\u672c\u7c7b\u578b\u4ece\u6839\u672c\u4e0a\u5f71\u54cd\u4e86\u68c0\u6d4b\u7ed3\u679c\u662f\u5426\u53ef\u9760\u3002<\/p>\n<\/div><\/div><\/div>
\n1. \u7b2c\u4e00\u6b21\u68c0\u6d4b\u4f7f\u7528\u7684\u662f\u5426\u4e3a\u8840\u6db2\u6837\u672c<\/p>\n<\/div><\/h4><\/div>
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\u6839\u636eNCCN\u975e\u5c0f\u7ec6\u80de\u80ba\u764c\u8bca\u7597\u6307\u5357\u4e2d\u7684\u7ecf\u9a8c\u7edf\u8ba1\uff0c\u8840\u6db2\u6837\u672c\u7684\u5047\u9634\u6027\uff08\u5373\u6f0f\u68c0\uff09\u6982\u7387\u7ea630%\u5de6\u53f3\uff0c\u537310\u540d\u5b9e\u9645\u6709\u7a81\u53d8\u7684\u60a3\u8005\u4e2d\u5c31\u7ea63\u540d\u60a3\u8005\u88ab\u6f0f\u6389[1]\u3002<\/p>\n
2020\u5e74\uff0c\u5929\u6d25\u533b\u79d1\u5927\u5b66\u53d1\u8868\u7814\u7a76\u56de\u987e2018\u5e74\u81f32021\u5e74\u671f\u95f4\u5929\u6d25\u533b\u79d1\u5927\u5b66\u603b\u533b\u9662\u80bf\u7624\u79d1\u6536\u6cbb\u7684NSCLC\u60a3\u8005\uff0c\u5176\u4e2d\u7eb3\u5165\u4e8632\u540d\u65e2\u6709\u7ec4\u7ec7\u6837\u672c\u4e5f\u6709\u8840\u6db2\u6837\u672c\u7684\u60a3\u8005\u5bf9\u6bd4\u4e24\u79cd\u6837\u672c\u7684\u68c0\u6d4b\u7ed3\u679c\uff0c\u572832\u4f8bNSCLC\u60a3\u8005\u4e2d\uff0c\u7ec4\u7ec7\u4e2d\u68c0\u6d4b\u51fa15\u4f8bEGFR\u7a81\u53d8\uff0c\u8840\u6d46\u4e2d\u68c0\u6d4b\u51fa13\u4f8b\u7a81\u53d8\uff08\u5373\u6709\u4e24\u540d\u5b9e\u9645\u6709EGFR\u7a81\u53d8\u7684\u60a3\u8005\u672a\u88ab\u8840\u6db2\u68c0\u6d4b\u51fa\uff09\uff0c\u7a81\u53d8\u4f4d\u70b9\u5b8c\u5168\u4e00\u81f4\u7684\u60a3\u8005\u517110\u4f8b\uff0c5\u4f8b\u60a3\u8005\u7ec4\u7ec7\u4e2d\u68c0\u6d4b\u51fa\u7684EGFR\u7a81\u53d8\u672a\u5728\u8840\u6d46\u4e2d\u68c0\u6d4b\u51fa\uff0c\u7ec4\u7ec7\u4e2d\u672a\u68c0\u6d4b\u5230\u7a81\u53d8\u7684\u8840\u6d46\u4e2d\u5747\u672a\u68c0\u6d4b\u5230\uff0c\u654f\u611f\u6027\u4e3a66.7%\uff0810\/15\uff09\uff0c\u7279\u5f02\u6027\u4e3a100%\uff0817\/17\uff09\uff0c\u4e24\u79cd\u6807\u672c\u68c0\u6d4b\u4e00\u81f4\u6027\u4e3a84.4%\uff0827\/32\uff09[2]\u3002\u7b80\u5355\u6765\u8bf4\uff0c\u8840\u6db2\u4e0d\u4ec5\u6709\u53ef\u80fd\u5c06\u5b9e\u9645\u6709\u7a81\u53d8\u7684\u60a3\u8005\u6f0f\u6389\uff0c\u8fd8\u6709\u53ef\u80fd\u68c0\u6d4b\u5230\u7684\u7a81\u53d8\u4f4d\u70b9\u5c11\u4e8e\u7ec4\u7ec7\u6837\u672c\u3002<\/p>\n
\u5b9e\u9645\u6cbb\u7597\u8fc7\u7a0b\u4e2d\uff0c\u5f88\u591a\u75c5\u53cb\u53ef\u80fd\u7531\u4e8e\u7126\u6025\u3001\u7f3a\u4e4f\u77e5\u8bc6\u3001\u6216\u8005\u60e7\u6015\u7a7f\u523a\u3001\u6c14\u7ba1\u955c\u7b49\u6d3b\u68c0\u624b\u6bb5\u800c\u4e0d\u613f\u53d6\u7ec4\u7ec7\uff0c\u4e8e\u662f\u9009\u62e9\u4e86\u7528\u8840\u6db2\u6837\u672c\u68c0\u6d4b\uff0c\u56e0\u6b64\u82e5\u57fa\u56e0\u68c0\u6d4b\u7ed3\u679c\u4e3a\u9634\u6027\u7684\u75c5\u53cb\u5728\u786e\u8ba4\u81ea\u8eab\u764c\u79cd\u662f\u5426\u6709\u9776\u5411\u836f\u4e4b\u540e\uff0c\u7b2c\u4e00\u70b9\u9700\u8981\u786e\u8ba4\u7684\u5c31\u662f\u6837\u672c\u7c7b\u578b\uff0c\u662f\u5426\u9009\u9519\u4e86\u8840\u6db2\u6837\u672c\uff0c\u5982\u679c\u786e\u5b9e\u4f7f\u7528\u7684\u662f\u8840\u6db2\u6837\u672c\uff0c\u5219\u5efa\u8bae\u6709\u6761\u4ef6\u7684\u60c5\u51b5\u4e0b\u53ef\u4ee5\u91cd\u65b0\u53d6\u7ec4\u7ec7\u68c0\u6d4b\u786e\u8ba4\u4e00\u6b21\u3002<\/p>\n<\/div>
![](\"https:\/\/www.funcbio.cn\/wp-content\/uploads\/2023\/11\/7-2.jpg\" "\\"7-2\\"")
<\/span><\/div>2. \u7b2c\u4e00\u6b21\u68c0\u6d4b\u4f7f\u7528\u7684\u7ec4\u7ec7\u6837\u672c\u662f\u5426\u7b26\u5408\u8d28\u63a7\u8981\u6c42<\/div><\/h4><\/div>\u672c\u4eba\u4ee5\u524d\u7684\u6587\u7ae0\u4e2d\u4e5f\u63d0\u53ca\u8fc7\u7ec4\u7ec7\u6837\u672c\u7684\u8d28\u91cf\u63a7\u5236\uff0c\u901a\u5e38\u7528\u4e8e\u57fa\u56e0\u68c0\u6d4b\u7684\u7ec4\u7ec7\u6837\u672c\u9700\u8981\u80bf\u7624\u6210\u5206\u542b\u91cf\u536020%\u4ee5\u4e0a\uff0c\u4e00\u822c\u68c0\u6d4b\u516c\u53f8\u9700\u898110-15\u5f20\u4e0d\u67d3\u8272\u7684\u5207\u7247\uff0c\u540c\u65f6\u5bf9\u4e8e\u4ece\u8fd9\u4e9b\u5207\u7247\u4e2d\u63d0\u53d6\u5230\u7684DNA\u603b\u91cf\u4e5f\u6709\u57fa\u672c\u8981\u6c42\uff0c\u5927\u81f4\u5982\u4e0b\u3002<\/p>\n<\/div>
![](\"https:\/\/www.funcbio.cn\/wp-content\/uploads\/2023\/11\/7-3.png\" "\\"7-3\\"")
<\/span><\/div>\u5927\u591a\u6570\u60c5\u51b5\u4e0b\u4f7f\u7528\u7ec4\u7ec7\u6837\u672c\u68c0\u6d4b\u5f88\u5c11\u4f1a\u6709\u9519\u6f0f\uff0c\u4f46\u4ecd\u5b58\u5728\u5c0f\u6982\u7387\u610f\u5916\u60c5\u51b5\uff0c\u901a\u5e38\u662f\u7531\u4e8e\u7126\u6025\u5fc3\u7406\uff0c\u8d2a\u56fe\u201c\u5feb\u201d\u5b57\u60f3\u5c3d\u5feb\u62ff\u5230\u7ed3\u679c\u5f00\u59cb\u6cbb\u7597\uff0c\u505a\u7a7f\u523a\u6216\u8005\u6c14\u7ba1\u955c\u7684\u540c\u65f6\u53d6\u4e24\u4efd\uff0c\u4e00\u4efd\u9001\u75c5\u7406\u79d1\u505a\u75c5\u7406\u8bca\u65ad\uff0c\u4e00\u4efd\u9001\u68c0\u6d4b\u516c\u53f8\uff0c\u6216\u8005\u5e72\u8106\u53ea\u53d6\u4e00\u4efd\u7ec4\u7ec7\u76f4\u63a5\u9001\u68c0\u6d4b\u516c\u53f8\u3002\u8fd9\u6837\u5c31\u7ed9\u68c0\u6d4b\u5e26\u6765\u4e86\u4e0d\u786e\u5b9a\u6027\u3002<\/p>\n
\u9996\u5148\u8fd8\u662f\u764c\u79cd\u7684\u95ee\u9898\uff0c\u5404\u4f4d\u4e00\u5b9a\u8981\u8bb0\u4f4f\u5e76\u975e\u6240\u6709\u764c\u79cd\u90fd\u6709\u5bf9\u5e94\u9776\u5411\u836f\uff0c\u5e76\u975e\u90fd\u5fc5\u987b\u57fa\u56e0\u68c0\u6d4b\uff1b\u5176\u6b21\u662f\u7a7f\u523a\u6216\u8005\u6c14\u7ba1\u955c\u5e76\u975e\u6bcf\u6b21\u90fd\u80fd\u53d6\u5230\u60f3\u8981\u7684\u80bf\u7624\u7ec4\u7ec7\uff0c\u6709\u53ef\u80fd\u53ea\u662f\u708e\u75c7\u7ec6\u80de\u6216\u8005\u6781\u5c11\u91cf\u80bf\u7624\u7ec6\u80de\uff0c\u56e0\u6b64\u8fd9\u79cd\u201c\u4e0d\u7b49\u75c5\u7406\u62a5\u544a\u5c31\u76f4\u63a5\u9001\u65b0\u9c9c\u7ec4\u7ec7\u505a\u57fa\u56e0\u68c0\u6d4b\u201d\u7684\u60c5\u51b5\uff0c\u82e5\u68c0\u6d4b\u9633\u6027\uff0c\u6709\u5bf9\u5e94\u9776\u5411\u836f\u90a3\u6700\u597d\uff0c\u82e5\u68c0\u6d4b\u9634\u6027\uff0c\u5219\u4e0d\u80fd\u786e\u5b9a\u662f\u56e0\u4e3a\u81ea\u8eab\u764c\u79cd\u6ca1\u6709\u9776\u5411\u836f\u5bfc\u81f4\u68c0\u6d4b\u4e0d\u5230\u9776\u5411\u57fa\u56e0\u7a81\u53d8\uff0c\u8fd8\u662f\u56e0\u4e3a\u53d6\u6837\u91cf\u4e0d\u591f\u6837\u672c\u8d28\u91cf\u4e0d\u8fc7\u5173\u5bfc\u81f4\u6f0f\u68c0\u3002<\/p>\n
\u7ec4\u7ec7\u6837\u672c\u5e76\u975e\u4e07\u65e0\u4e00\u5931\uff0c\u82e5\u4f7f\u7528\u7ec4\u7ec7\u6837\u672c\u68c0\u6d4b\u4e5f\u4e3a\u9634\u6027\uff0c\u9700\u5ba1\u89c6\u4e00\u4e0b\u81ea\u8eab\u662f\u5426\u72af\u4e86\u4e0a\u8ff0\u7126\u6025\u5f15\u8d77\u7684\u9519\u8bef\uff0c\u4e07\u4e00\u7b26\u5408\u5219\u53ef\u8003\u8651\u91cd\u65b0\u53d6\u6837\u6216\u8005\u7b49\u75c5\u7406\u8bca\u65ad\u51fa\u6765\u4e4b\u540e\u518d\u6b21\u68c0\u6d4b\u3002<\/p>\n
\uff08\u6ce8\u610f\uff1a\u901a\u5e38\u75c5\u7406\u62a5\u544a3-5\u4e2a\u5de5\u4f5c\u65e5\u5c31\u6709\u7ed3\u679c\uff0c\u9664\u4e86\u786e\u8bca\u764c\u79cd\u4ee5\u5916\uff0c\u8fd8\u53ef\u4ee5\u5927\u81f4\u4e86\u89e3\u6837\u672c\u4e2d\u80bf\u7624\u542b\u91cf\u591a\u5c11\uff0c\u4e0d\u4f1a\u803d\u8bef\u5404\u4f4d\u592a\u591a\u65f6\u95f4\u53cd\u800c\u8fd8\u80fd\u5e2e\u52a9\u4e86\u89e3\u6837\u672c\u8d28\u91cf\u597d\u574f\uff0c\u5c3d\u91cf\u4e0d\u8981\u7701\u7565\uff09<\/p>\n<\/div>
![](\"https:\/\/www.funcbio.cn\/wp-content\/uploads\/2023\/11\/7-4.jpg\" "\\"7-4\\"")
<\/span><\/div><\/div><\/div>\u5f71\u54cd\u56e0\u7d20\u4e09\uff1a\u68c0\u6d4b\u6280\u672f<\/div><\/h4><\/div>\u5f88\u591a\u5584\u4e8e\u5b66\u4e60\u7684\u75c5\u53cb\u5bf9\u4e8e\u68c0\u6d4b\u6280\u672f\u4e5f\u6709\u6240\u4e86\u89e3\uff0c\u5bf9\u4e8ePCR\u548cNGS\u6280\u672f\u5e76\u4e0d\u964c\u751f\uff0c\u6211\u4e4b\u524d\u4e5f\u5199\u8fc7\u300a\u9662\u5185\u9662\u5916\u57fa\u56e0\u68c0\u6d4b\u7684\u533a\u522b\u300b\uff08\u70b9\u51fb\u9605\u8bfb\uff09\uff0c\u5927\u90e8\u5206\u533b\u9662\u9662\u5185\u5747\u91c7\u7528PCR\u6280\u672f\uff0c\u65b9\u4fbf\u5feb\u6377\u4e14\u57fa\u672c\u80fd\u591f\u8986\u76d6\u9664NTRK\u4ee5\u5916\u7684\u5176\u4ed6\u9776\u5411\u57fa\u56e0\uff0c\u53ef\u4ee5\u6ee1\u8db3\u5927\u591a\u6570\u60a3\u8005\u7684\u68c0\u6d4b\u9700\u6c42\uff0c\u4e0d\u8fc7PCR\u6280\u672f\u5b58\u5728\u4e00\u5b9a\u7f3a\u9677\uff0c\u53ef\u80fd\u9020\u6210\u6781\u5c0f\u6982\u7387\u9057\u6f0f\u3002<\/p>\n<\/div>
\n1.PCR\u6280\u672f\u96be\u4ee5\u8986\u76d6\u9776\u5411\u57fa\u56e0\u7684\u7f55\u89c1\u7a81\u53d8\u578b<\/p>\n<\/div><\/h4><\/div>
\u901a\u4fd7\u4e00\u70b9\u8bf4\uff0cPCR\u6280\u672f\u5c31\u50cf\u662f\u62ff\u7740\u73b0\u6210\u7684\u6863\u6848\u53bb\u6392\u67e5\u5acc\u7591\u4eba\uff0c\u800cNGS\u6280\u672f\u5219\u662f\u5730\u6bef\u5f0f\u641c\u67e5\uff0c\u65e0\u8bba\u662f\u6863\u6848\u91cc\u7684\u8fd8\u662f\u6863\u6848\u5916\u7684\uff0c\u53ea\u8981\u68c0\u67e5\u53d1\u73b0\u6709\u95ee\u9898\u7684\u901a\u901a\u4e0a\u62a5\u3002PCR\u6280\u672f\u53ef\u7528\u4e8e\u68c0\u6d4b\u70b9\u7a81\u53d8\u4e0e\u878d\u5408\u7a81\u53d8\uff0c\u53ef\u8986\u76d6\u76ee\u6807\u57fa\u56e0\u7684\u5404\u79cd\u5df2\u77e5\u7a81\u53d8\u7c7b\u578b\uff0c\u4f46\u5bf9\u4e8e\u672a\u77e5\u53ca\u7f55\u89c1\u7a81\u53d8\u4f4d\u70b9\u5219\u7565\u663e\u65e0\u529b\u3002<\/p>\n
\u4ee5\u6700\u5e38\u89c1\u7684EGFR\u57fa\u56e0\u68c0\u6d4b\u4e3a\u4f8b\uff0c\u76ee\u524d\u56fd\u5185\u5df2\u83b7\u6279\u7684PCR\u8bd5\u5242\u76d2\u7684\u68c0\u6d4b\u4f4d\u70b9\u4e3b\u8981\u5305\u62ec\u5404\u7c7bEGFR 19\u7f3a\u5931\u7a81\u53d8\u3001EGFR L858R\u7a81\u53d8\u3001EGFR T790M\u7a81\u53d8\uff0cEGFR 20\u63d2\u5165\u4ee5\u53ca\u90e8\u5206\u4f4d\u70b9\u3002<\/p>\n
\u867d\u7136\u8fd9\u4e9b\u5df2\u7ecf\u8986\u76d6\u4e86\u8d85\u8fc790%\u7684EGFR\u7a81\u53d8\uff0c\u4f46EGFR\u57fa\u56e0\u768418\u300119\u300120\u300121\u5916\u663e\u5b50\u5185\u4ecd\u53ef\u80fd\u51fa\u73b0\u5982EGFR C797S\u3001EGFR L833V\u3001EGFR L747Q\u7b49\u7b49\u66f4\u52a0\u5c11\u89c1\u7684\u7a81\u53d8\u4f4d\u70b9\u3002\u8fd9\u4e9b\u7a81\u53d8\u6216\u51fa\u73b0\u7684\u6982\u7387\u6781\u4f4e\uff08\u5373\u7f55\u89c1\u7a81\u53d8\u4f4d\u70b9\uff09\u6216\u7279\u6b8a\u60c5\u51b5\u4e0b\u53ef\u80fd\u51fa\u73b0\uff0c\u53ef\u80fd\u4ec5\u5360\u6240\u6709EGFR\u7a81\u53d8\u76841-5%\u5de6\u53f3\uff08\u8f83\u96be\u6709\u5b8c\u5168\u7edf\u8ba1\uff09\uff0c\u5bf9\u4e8e\u8fd9\u4e9b\u4e0d\u5728\u68c0\u6d4b\u8303\u56f4\u5185\u7684\u7a81\u53d8\u4f4d\u70b9\uff0cPCR\u6280\u672f\u65e0\u80fd\u4e3a\u529b\uff0c\u4f46NGS\u6280\u672f\u5219\u6709\u53ef\u80fd\u68c0\u51fa\u3002<\/p>\n<\/div>
![](\"https:\/\/www.funcbio.cn\/wp-content\/uploads\/2023\/11\/7-6.png\" "\\"7-6\\"")
<\/span><\/div>\u518d\u4ee5\u88ab\u79f0\u4e3a\u201c\u94bb\u77f3\u7a81\u53d8\u201d\u7684ALK\u878d\u5408\u7a81\u53d8\u4e3a\u4f8b\uff0c\u8be5\u7a81\u53d8\u7c7b\u578b\u4e0d\u540c\u4e8e\u4e0a\u8ff0\u7684EGFR\u70b9\u7a81\u53d8\uff0c\u5e76\u4e0d\u662f\u5355\u4e2a\u57fa\u56e0\u7684\u4e8b\uff0c\u5b83\u662f\u4e24\u4e2a\u57fa\u56e0\u95f4\u7684\u65ad\u88c2\u4e0e\u878d\u5408\uff0c\u6211\u4eec\u5c06\u8fd9\u79cd\u878d\u5408\u7684\u4e24\u4e2a\u57fa\u56e0\u6210\u4e3a\u201c\u878d\u5408\u4f34\u4fa3\u201d\uff0cALK\u878d\u5408\u5373\u662f\u6307\u5176\u4e2d\u4e00\u4e2a\u4f34\u4fa3\u56fa\u5b9a\u4e3aALK\u57fa\u56e0\uff0c\u53e6\u4e00\u534a\u53ef\u80fd\u4f1a\u6709\u591a\u79cd\u3002<\/p>\n
\u6700\u5e38\u89c1\u7684ALK\u878d\u5408\u53d1\u751f\u5728EML4\u4e0eALK\u57fa\u56e0\u4e4b\u95f4\uff0c\u4f46\u968f\u7740\u7814\u7a76\u53d1\u5c55\uff0c\u6211\u4eec\u53d1\u73b0\u4e86\u66f4\u591a\u4f34\u4fa3\u57fa\u56e0\uff0c\u8f83\u65e9\u4ee5\u524d\u7684PCR\u8bd5\u5242\u76d2\u4ec5\u53ef\u68c0\u6d4bEML4-ALK\u878d\u5408\u7c7b\u578b\uff0c\u5176\u4ed6\u878d\u5408\u4f34\u4fa3\u5f62\u6210\u7684ALK\u878d\u5408\u5219\u65e0\u6cd5\u53d1\u73b0\uff0c\u73b0\u5728\u6709\u4e9b\u66f4\u65b0\u7684PCR\u8bd5\u5242\u76d2\u9664EML4\u57fa\u56e0\u4ee5\u5916\uff0c\u8fd8\u5305\u542bKIF5B\u3001KCL1\u3001TFG\u7b49\u6982\u7387\u76f8\u5bf9\u8f83\u4f4e\u7684\u878d\u5408\u4f34\u4fa3\uff0c\u8fdb\u4e00\u6b65\u6269\u5927\u4e86PCR\u6280\u672f\u53ef\u68c0\u6d4b\u5230\u7684ALK\u878d\u5408\u7c7b\u578b\u3002<\/p>\n
\u53ef\u4ee5\u8bf490%\u751a\u81f395%\u4ee5\u4e0a\u7684ALK\u878d\u5408\u60a3\u8005\u90fd\u53ef\u901a\u8fc7PCR\u6280\u672f\u53d1\u73b0\uff0c\u4f46ALK\u57fa\u56e0\u7684\u878d\u5408\u4f34\u4fa3\u4ecd\u7136\u8d85\u51fa\u6b64\u8303\u56f4\uff0c\u4f8b\u5982\u6211\u66fe\u9047\u5230\u8fc7\u67d0\u4e2a\u75c5\u53cb\u662fDIRC3-ALK\u878d\u5408\uff0cNGS\u6280\u672f\u7684\u7279\u6027\u8ba9\u5b83\u4e0d\u53d7\u8fd9\u79cd\u8303\u56f4\u9650\u5236\uff0c\u65e0\u8bba\u54ea\u79cd\u878d\u5408\u4f34\u4fa3\uff0c\u7406\u8bba\u4e0a\u90fd\u53ef\u4ee5\u68c0\u51fa\uff0c\u907f\u514d\u8fd9\u79cd\u5c0f\u6982\u7387\u610f\u5916\u3002<\/p>\n<\/div>
![](\"https:\/\/www.funcbio.cn\/wp-content\/uploads\/2023\/11\/7-7.png\" "\\"7-7\\"")
<\/span><\/div>\n2. PCR\u6280\u672f\u80fd\u4ee5\u8986\u76d6\u90e8\u5206\u7a81\u53d8\u7c7b\u578b<\/p>\n<\/div><\/h4><\/div>
PCR\u6280\u672f\u5bf9\u4e8e\u68c0\u6d4b\u70b9\u7a81\u53d8\u3001\u878d\u5408\u7a81\u53d8\u7684\u68c0\u6d4b\u80fd\u529b\u8f83\u5f3a\uff0c\u4f46\u76ee\u524d\u5404\u533b\u9662\u5e38\u7528\u7684PCR\u68c0\u6d4b\u8bd5\u5242\u76d2\u5bf9\u4e8e\u201c\u6269\u589e\u201d\u8fd9\u4e00\u7a81\u53d8\u7c7b\u578b\u57fa\u672c\u6ca1\u6709\u8986\u76d6\uff0c\u800c\u643a\u5e26HER2\u6269\u589e\u6216\u8005MET\u6269\u589e\u7684\u975e\u5c0f\u7ec6\u80de\u80ba\u764c\u60a3\u8005\u5206\u522b\u5360\u5230\u4e86\u6574\u4f53\u4eba\u7fa4\u76841-3%\uff0c\u82e5\u4f7f\u7528PCR\u6280\u672f\u53ef\u80fd\u5bfc\u81f4\u8fd9\u4e9b\u53ef\u7528\u836f\u7684\u9a71\u52a8\u57fa\u56e0\u7a81\u53d8\u7684\u60a3\u8005\u4f1a\u88ab\u9057\u6f0f\u3002<\/p>\n<\/div>
![](\"https:\/\/www.funcbio.cn\/wp-content\/uploads\/2023\/11\/7-8.png\" "\\"7-8\\"")
<\/span><\/div><\/div><\/div>\u5404\u4f4d\u53ef\u901a\u8fc7\u68c0\u67e5\u81ea\u5df1\u62ff\u5230\u7684\u533b\u9662\u7684\u68c0\u6d4b\u62a5\u544a\u4e0a\u68c0\u6d4b\u6280\u672f\u680f\u662f\u5426\u6709\u201c\u8367\u5149\u5b9a\u91cfPCR\u201d\u3001\u201cRT-PCR\u201d\u4ee5\u53ca\u201cARMS\u201d\u7b49\u5b57\u6837\uff0c\u82e5\u6709\u7684\u8bdd\u5219\u4e3aPCR\u6280\u672f\u3002\u5982\u679c\u5404\u4f4d\u4e00\u6b65\u4e00\u6b65\u6392\u67e5\u81ea\u8eab\u68c0\u6d4b\u6d41\u7a0b\uff0c\u786e\u8ba4\u81ea\u8eab\u764c\u79cd\u6709\u9776\u5411\u836f\u4e14\u4f7f\u7528\u4e86\u6b63\u786e\u5408\u683c\u7684\u7ec4\u7ec7\u6837\u672c\uff0c\u6700\u540e\u4f7f\u7528PCR\u6280\u672f\u68c0\u6d4b\u4e3a\u9634\u6027\uff0c\u90a3\u4e48\u6709\u6761\u4ef6\u7684\u5bb6\u5ead\u5728\u5269\u4f59\u6837\u672c\u5145\u8db3\u4ee5\u53ca\u7ecf\u6d4e\u5141\u8bb8\u7684\u60c5\u51b5\u4e0b\uff0c\u53ef\u4ee5\u8003\u8651\u518d\u5c1d\u8bd5NGS\u68c0\u6d4b\uff0c\u770b\u81ea\u8eab\u662f\u5426\u5c5e\u4e8e\u4e0a\u8ff0\u5c0f\u6982\u7387\u7f55\u89c1\u7a81\u53d8\u7c7b\u578b\u3002<\/p>\n<\/div>
\u603b\u7ed3<\/div><\/h4><\/div>\u6700\u540e\u5e2e\u5404\u4f4d\u68b3\u7406\u4e00\u4e0b\u82e5\u57fa\u56e0\u68c0\u6d4b\u62a5\u544a\u9634\u6027\uff0c\u4ece\u57fa\u56e0\u68c0\u6d4b\u89d2\u5ea6\uff0c\u8fd8\u53ef\u4ee5\u505a\u54ea\u4e9b\u5c1d\u8bd5\uff1a<\/p>\n
1. \u56de\u5f52\u75c5\u7406\u62a5\u544a\uff0c\u786e\u8ba4\u81ea\u8eab\u764c\u79cd\uff0c\u4e86\u89e3\u81ea\u8eab\u764c\u79cd\u662f\u5426\u6709\u5bf9\u5e94\u9776\u5411\u836f\u4ee5\u53ca\u9776\u5411\u57fa\u56e0\u7a81\u53d8\u53ef\u80fd\u6027\u5927\u5c0f\uff0c\u82e5\u65e0\u76f8\u5173\u9776\u5411\u836f\uff0c\u5219\u518d\u6b21\u57fa\u56e0\u68c0\u6d4b\u610f\u4e49\u4e0d\u5927\uff1b<\/p>\n
2. \u56de\u987e\u68c0\u6d4b\u4f7f\u7528\u7684\u6837\u672c\u7c7b\u578b\uff0c\u82e5\u4ec5\u4f7f\u7528\u8840\u6db2\u6837\u672c\u4e14\u672a\u68c0\u6d4b\u5230\u4efb\u4f55\u7a81\u53d8\uff0c\u5219\u6709\u6761\u4ef6\u7684\u60c5\u51b5\u4e0b\u53ef\u8003\u8651\u5c3d\u91cf\u83b7\u53d6\u7ec4\u7ec7\u6837\u672c\uff0c\u5728\u786e\u5b9a\u764c\u79cd\u7c7b\u578b\u9002\u5408\u540e\u7528\u7ec4\u7ec7\u6837\u672c\u518d\u884c\u68c0\u6d4b\uff1b<\/p>\n
3. \u56de\u987e\u68c0\u6d4b\u4f7f\u7528\u7684\u6837\u672c\u7c7b\u578b\uff0c\u82e5\u4f7f\u7528\u7684\u4e3a\u65b0\u9c9c\u6807\u672c\u6216\u6837\u672c\u91cf\u4e0d\u8db3\u7684\u77f3\u8721\u5207\u7247\uff0c\u5219\u4ecd\u662f\u56de\u5f52\u75c5\u7406\u62a5\u544a\uff0c\u5148\u786e\u8ba4\u6b64\u6b21\u53d6\u6837\u662f\u5426\u53d6\u5230\u80bf\u7624\u4ee5\u53ca\u80bf\u7624\u542b\u91cf\u662f\u5426\u8fbe\u6807\uff0c\u82e5\u4e0d\u7b26\u5408\u5219\u53ef\u8003\u8651\u91cd\u65b0\u53d6\u6837\uff0c\u8fbe\u6807\u540e\u518d\u9001\u68c0\uff0c\u82e5\u7b26\u5408\u6807\u51c6\u5219\u7ed3\u679c\u57fa\u672c\u53ef\u4fe1\uff0c\u518d\u884c\u68c0\u6d4b\u6539\u53d8\u53ef\u80fd\u6027\u4e0d\u5927\uff1b<\/p>\n
4. \u5ba1\u89c6\u68c0\u6d4b\u6280\u672f\uff0c\u82e5\u764c\u79cd\u3001\u6837\u672c\u5747\u65e0\u95ee\u9898\uff0c\u4f7f\u7528\u7684\u662fPCR\u6280\u672f\u68c0\u6d4b\uff0c\u7ecf\u6d4e\u5141\u8bb8\u4e14\u5269\u4f59\u6837\u672c\u8db3\u591f\u7684\u60c5\u51b5\u4e0b\u53ef\u8003\u8651\u518d\u884cNGS\u6280\u672f\u68c0\u6d4b\uff0c\u4ee5\u514d\u9057\u6f0f\u5c0f\u6982\u7387\u7684\u7f55\u89c1\u7a81\u53d8\u4f4d\u70b9\u53ca\u7c7b\u578b\u3002<\/p>\n
5. \u82e5\u764c\u79cd\u3001\u6837\u672c\uff0c\u8fd8\u6709\u68c0\u6d4b\u6280\u672f\u5747\u65e0\u95ee\u9898\uff0c\u90a3\u4e48\u57fa\u56e0\u68c0\u6d4b\u7ed3\u679c\u95ee\u9898\u4e0d\u5927\uff0c\u57fa\u672c\u4e0d\u4f1a\u6709\u9519\u6f0f\uff0c\u77ed\u671f\u5185\u4e0d\u9700\u8981\u4e8c\u6b21\u68c0\u6d4b\uff0c\u6539\u53d8\u53ef\u80fd\u6027\u4e5f\u4e0d\u9ad8\uff0c\u5b9e\u5728\u60f3\u640f\u4e00\u640f\u5efa\u8bae\u5728\u63a5\u53d7\u6b63\u89c4\u6cbb\u7597\u51fa\u73b0\u8fdb\u5c55\uff0c\u60c5\u51b5\u6709\u53d8\u5316\u4e4b\u540e\u624d\u8003\u8651\u3002<\/p>\n
\u672c\u6587\u7ae0\u8f6c\u8f7d\u4e8e\uff1a \u4e0e\u7231\u5171\u821e\u8ba2\u9605\u53f7<\/p>\n
\u539f\u6587\u94fe\u63a5\uff1ahttps:\/\/mp.weixin.qq.com\/s\/w6yaj-CImt27KZFIDDHHQw<\/p>\n<\/div><\/div><\/div><\/div><\/div><\/p>","protected":false},"excerpt":{"rendered":"
Patients who have just been diagnosed with non-small cell lung cancer have raised a common question: \"What should I do if the first genetic test shows no mutation? Do I need to retest?\" Regarding this issue, I would like to propose the following ideas from the perspective of genetic testing for your reference.<\/p>","protected":false},"author":1,"featured_media":3606,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"content-type":"","om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[202],"tags":[],"class_list":["post-1323","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-202"],"acf":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/posts\/1323","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/comments?post=1323"}],"version-history":[{"count":6,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/posts\/1323\/revisions"}],"predecessor-version":[{"id":3618,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/posts\/1323\/revisions\/3618"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/media\/3606"}],"wp:attachment":[{"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/media?parent=1323"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/categories?post=1323"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/tags?post=1323"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
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2020\u5e74\uff0c\u5929\u6d25\u533b\u79d1\u5927\u5b66\u53d1\u8868\u7814\u7a76\u56de\u987e2018\u5e74\u81f32021\u5e74\u671f\u95f4\u5929\u6d25\u533b\u79d1\u5927\u5b66\u603b\u533b\u9662\u80bf\u7624\u79d1\u6536\u6cbb\u7684NSCLC\u60a3\u8005\uff0c\u5176\u4e2d\u7eb3\u5165\u4e8632\u540d\u65e2\u6709\u7ec4\u7ec7\u6837\u672c\u4e5f\u6709\u8840\u6db2\u6837\u672c\u7684\u60a3\u8005\u5bf9\u6bd4\u4e24\u79cd\u6837\u672c\u7684\u68c0\u6d4b\u7ed3\u679c\uff0c\u572832\u4f8bNSCLC\u60a3\u8005\u4e2d\uff0c\u7ec4\u7ec7\u4e2d\u68c0\u6d4b\u51fa15\u4f8bEGFR\u7a81\u53d8\uff0c\u8840\u6d46\u4e2d\u68c0\u6d4b\u51fa13\u4f8b\u7a81\u53d8\uff08\u5373\u6709\u4e24\u540d\u5b9e\u9645\u6709EGFR\u7a81\u53d8\u7684\u60a3\u8005\u672a\u88ab\u8840\u6db2\u68c0\u6d4b\u51fa\uff09\uff0c\u7a81\u53d8\u4f4d\u70b9\u5b8c\u5168\u4e00\u81f4\u7684\u60a3\u8005\u517110\u4f8b\uff0c5\u4f8b\u60a3\u8005\u7ec4\u7ec7\u4e2d\u68c0\u6d4b\u51fa\u7684EGFR\u7a81\u53d8\u672a\u5728\u8840\u6d46\u4e2d\u68c0\u6d4b\u51fa\uff0c\u7ec4\u7ec7\u4e2d\u672a\u68c0\u6d4b\u5230\u7a81\u53d8\u7684\u8840\u6d46\u4e2d\u5747\u672a\u68c0\u6d4b\u5230\uff0c\u654f\u611f\u6027\u4e3a66.7%\uff0810\/15\uff09\uff0c\u7279\u5f02\u6027\u4e3a100%\uff0817\/17\uff09\uff0c\u4e24\u79cd\u6807\u672c\u68c0\u6d4b\u4e00\u81f4\u6027\u4e3a84.4%\uff0827\/32\uff09[2]\u3002\u7b80\u5355\u6765\u8bf4\uff0c\u8840\u6db2\u4e0d\u4ec5\u6709\u53ef\u80fd\u5c06\u5b9e\u9645\u6709\u7a81\u53d8\u7684\u60a3\u8005\u6f0f\u6389\uff0c\u8fd8\u6709\u53ef\u80fd\u68c0\u6d4b\u5230\u7684\u7a81\u53d8\u4f4d\u70b9\u5c11\u4e8e\u7ec4\u7ec7\u6837\u672c\u3002<\/p>\n
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<\/span><\/div>2. \u7b2c\u4e00\u6b21\u68c0\u6d4b\u4f7f\u7528\u7684\u7ec4\u7ec7\u6837\u672c\u662f\u5426\u7b26\u5408\u8d28\u63a7\u8981\u6c42<\/div><\/h4><\/div>\u672c\u4eba\u4ee5\u524d\u7684\u6587\u7ae0\u4e2d\u4e5f\u63d0\u53ca\u8fc7\u7ec4\u7ec7\u6837\u672c\u7684\u8d28\u91cf\u63a7\u5236\uff0c\u901a\u5e38\u7528\u4e8e\u57fa\u56e0\u68c0\u6d4b\u7684\u7ec4\u7ec7\u6837\u672c\u9700\u8981\u80bf\u7624\u6210\u5206\u542b\u91cf\u536020%\u4ee5\u4e0a\uff0c\u4e00\u822c\u68c0\u6d4b\u516c\u53f8\u9700\u898110-15\u5f20\u4e0d\u67d3\u8272\u7684\u5207\u7247\uff0c\u540c\u65f6\u5bf9\u4e8e\u4ece\u8fd9\u4e9b\u5207\u7247\u4e2d\u63d0\u53d6\u5230\u7684DNA\u603b\u91cf\u4e5f\u6709\u57fa\u672c\u8981\u6c42\uff0c\u5927\u81f4\u5982\u4e0b\u3002<\/p>\n<\/div>
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<\/span><\/div><\/div><\/div>\u5f71\u54cd\u56e0\u7d20\u4e09\uff1a\u68c0\u6d4b\u6280\u672f<\/div><\/h4><\/div>\u5f88\u591a\u5584\u4e8e\u5b66\u4e60\u7684\u75c5\u53cb\u5bf9\u4e8e\u68c0\u6d4b\u6280\u672f\u4e5f\u6709\u6240\u4e86\u89e3\uff0c\u5bf9\u4e8ePCR\u548cNGS\u6280\u672f\u5e76\u4e0d\u964c\u751f\uff0c\u6211\u4e4b\u524d\u4e5f\u5199\u8fc7\u300a\u9662\u5185\u9662\u5916\u57fa\u56e0\u68c0\u6d4b\u7684\u533a\u522b\u300b\uff08\u70b9\u51fb\u9605\u8bfb\uff09\uff0c\u5927\u90e8\u5206\u533b\u9662\u9662\u5185\u5747\u91c7\u7528PCR\u6280\u672f\uff0c\u65b9\u4fbf\u5feb\u6377\u4e14\u57fa\u672c\u80fd\u591f\u8986\u76d6\u9664NTRK\u4ee5\u5916\u7684\u5176\u4ed6\u9776\u5411\u57fa\u56e0\uff0c\u53ef\u4ee5\u6ee1\u8db3\u5927\u591a\u6570\u60a3\u8005\u7684\u68c0\u6d4b\u9700\u6c42\uff0c\u4e0d\u8fc7PCR\u6280\u672f\u5b58\u5728\u4e00\u5b9a\u7f3a\u9677\uff0c\u53ef\u80fd\u9020\u6210\u6781\u5c0f\u6982\u7387\u9057\u6f0f\u3002<\/p>\n<\/div>
\n1.PCR\u6280\u672f\u96be\u4ee5\u8986\u76d6\u9776\u5411\u57fa\u56e0\u7684\u7f55\u89c1\u7a81\u53d8\u578b<\/p>\n<\/div><\/h4><\/div>
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\u4ee5\u6700\u5e38\u89c1\u7684EGFR\u57fa\u56e0\u68c0\u6d4b\u4e3a\u4f8b\uff0c\u76ee\u524d\u56fd\u5185\u5df2\u83b7\u6279\u7684PCR\u8bd5\u5242\u76d2\u7684\u68c0\u6d4b\u4f4d\u70b9\u4e3b\u8981\u5305\u62ec\u5404\u7c7bEGFR 19\u7f3a\u5931\u7a81\u53d8\u3001EGFR L858R\u7a81\u53d8\u3001EGFR T790M\u7a81\u53d8\uff0cEGFR 20\u63d2\u5165\u4ee5\u53ca\u90e8\u5206\u4f4d\u70b9\u3002<\/p>\n
\u867d\u7136\u8fd9\u4e9b\u5df2\u7ecf\u8986\u76d6\u4e86\u8d85\u8fc790%\u7684EGFR\u7a81\u53d8\uff0c\u4f46EGFR\u57fa\u56e0\u768418\u300119\u300120\u300121\u5916\u663e\u5b50\u5185\u4ecd\u53ef\u80fd\u51fa\u73b0\u5982EGFR C797S\u3001EGFR L833V\u3001EGFR L747Q\u7b49\u7b49\u66f4\u52a0\u5c11\u89c1\u7684\u7a81\u53d8\u4f4d\u70b9\u3002\u8fd9\u4e9b\u7a81\u53d8\u6216\u51fa\u73b0\u7684\u6982\u7387\u6781\u4f4e\uff08\u5373\u7f55\u89c1\u7a81\u53d8\u4f4d\u70b9\uff09\u6216\u7279\u6b8a\u60c5\u51b5\u4e0b\u53ef\u80fd\u51fa\u73b0\uff0c\u53ef\u80fd\u4ec5\u5360\u6240\u6709EGFR\u7a81\u53d8\u76841-5%\u5de6\u53f3\uff08\u8f83\u96be\u6709\u5b8c\u5168\u7edf\u8ba1\uff09\uff0c\u5bf9\u4e8e\u8fd9\u4e9b\u4e0d\u5728\u68c0\u6d4b\u8303\u56f4\u5185\u7684\u7a81\u53d8\u4f4d\u70b9\uff0cPCR\u6280\u672f\u65e0\u80fd\u4e3a\u529b\uff0c\u4f46NGS\u6280\u672f\u5219\u6709\u53ef\u80fd\u68c0\u51fa\u3002<\/p>\n<\/div>
<\/span><\/div>\u518d\u4ee5\u88ab\u79f0\u4e3a\u201c\u94bb\u77f3\u7a81\u53d8\u201d\u7684ALK\u878d\u5408\u7a81\u53d8\u4e3a\u4f8b\uff0c\u8be5\u7a81\u53d8\u7c7b\u578b\u4e0d\u540c\u4e8e\u4e0a\u8ff0\u7684EGFR\u70b9\u7a81\u53d8\uff0c\u5e76\u4e0d\u662f\u5355\u4e2a\u57fa\u56e0\u7684\u4e8b\uff0c\u5b83\u662f\u4e24\u4e2a\u57fa\u56e0\u95f4\u7684\u65ad\u88c2\u4e0e\u878d\u5408\uff0c\u6211\u4eec\u5c06\u8fd9\u79cd\u878d\u5408\u7684\u4e24\u4e2a\u57fa\u56e0\u6210\u4e3a\u201c\u878d\u5408\u4f34\u4fa3\u201d\uff0cALK\u878d\u5408\u5373\u662f\u6307\u5176\u4e2d\u4e00\u4e2a\u4f34\u4fa3\u56fa\u5b9a\u4e3aALK\u57fa\u56e0\uff0c\u53e6\u4e00\u534a\u53ef\u80fd\u4f1a\u6709\u591a\u79cd\u3002<\/p>\n
\u6700\u5e38\u89c1\u7684ALK\u878d\u5408\u53d1\u751f\u5728EML4\u4e0eALK\u57fa\u56e0\u4e4b\u95f4\uff0c\u4f46\u968f\u7740\u7814\u7a76\u53d1\u5c55\uff0c\u6211\u4eec\u53d1\u73b0\u4e86\u66f4\u591a\u4f34\u4fa3\u57fa\u56e0\uff0c\u8f83\u65e9\u4ee5\u524d\u7684PCR\u8bd5\u5242\u76d2\u4ec5\u53ef\u68c0\u6d4bEML4-ALK\u878d\u5408\u7c7b\u578b\uff0c\u5176\u4ed6\u878d\u5408\u4f34\u4fa3\u5f62\u6210\u7684ALK\u878d\u5408\u5219\u65e0\u6cd5\u53d1\u73b0\uff0c\u73b0\u5728\u6709\u4e9b\u66f4\u65b0\u7684PCR\u8bd5\u5242\u76d2\u9664EML4\u57fa\u56e0\u4ee5\u5916\uff0c\u8fd8\u5305\u542bKIF5B\u3001KCL1\u3001TFG\u7b49\u6982\u7387\u76f8\u5bf9\u8f83\u4f4e\u7684\u878d\u5408\u4f34\u4fa3\uff0c\u8fdb\u4e00\u6b65\u6269\u5927\u4e86PCR\u6280\u672f\u53ef\u68c0\u6d4b\u5230\u7684ALK\u878d\u5408\u7c7b\u578b\u3002<\/p>\n
\u53ef\u4ee5\u8bf490%\u751a\u81f395%\u4ee5\u4e0a\u7684ALK\u878d\u5408\u60a3\u8005\u90fd\u53ef\u901a\u8fc7PCR\u6280\u672f\u53d1\u73b0\uff0c\u4f46ALK\u57fa\u56e0\u7684\u878d\u5408\u4f34\u4fa3\u4ecd\u7136\u8d85\u51fa\u6b64\u8303\u56f4\uff0c\u4f8b\u5982\u6211\u66fe\u9047\u5230\u8fc7\u67d0\u4e2a\u75c5\u53cb\u662fDIRC3-ALK\u878d\u5408\uff0cNGS\u6280\u672f\u7684\u7279\u6027\u8ba9\u5b83\u4e0d\u53d7\u8fd9\u79cd\u8303\u56f4\u9650\u5236\uff0c\u65e0\u8bba\u54ea\u79cd\u878d\u5408\u4f34\u4fa3\uff0c\u7406\u8bba\u4e0a\u90fd\u53ef\u4ee5\u68c0\u51fa\uff0c\u907f\u514d\u8fd9\u79cd\u5c0f\u6982\u7387\u610f\u5916\u3002<\/p>\n<\/div>
<\/span><\/div>\n2. PCR\u6280\u672f\u80fd\u4ee5\u8986\u76d6\u90e8\u5206\u7a81\u53d8\u7c7b\u578b<\/p>\n<\/div><\/h4><\/div>
PCR\u6280\u672f\u5bf9\u4e8e\u68c0\u6d4b\u70b9\u7a81\u53d8\u3001\u878d\u5408\u7a81\u53d8\u7684\u68c0\u6d4b\u80fd\u529b\u8f83\u5f3a\uff0c\u4f46\u76ee\u524d\u5404\u533b\u9662\u5e38\u7528\u7684PCR\u68c0\u6d4b\u8bd5\u5242\u76d2\u5bf9\u4e8e\u201c\u6269\u589e\u201d\u8fd9\u4e00\u7a81\u53d8\u7c7b\u578b\u57fa\u672c\u6ca1\u6709\u8986\u76d6\uff0c\u800c\u643a\u5e26HER2\u6269\u589e\u6216\u8005MET\u6269\u589e\u7684\u975e\u5c0f\u7ec6\u80de\u80ba\u764c\u60a3\u8005\u5206\u522b\u5360\u5230\u4e86\u6574\u4f53\u4eba\u7fa4\u76841-3%\uff0c\u82e5\u4f7f\u7528PCR\u6280\u672f\u53ef\u80fd\u5bfc\u81f4\u8fd9\u4e9b\u53ef\u7528\u836f\u7684\u9a71\u52a8\u57fa\u56e0\u7a81\u53d8\u7684\u60a3\u8005\u4f1a\u88ab\u9057\u6f0f\u3002<\/p>\n<\/div>
<\/span><\/div><\/div><\/div>\u5404\u4f4d\u53ef\u901a\u8fc7\u68c0\u67e5\u81ea\u5df1\u62ff\u5230\u7684\u533b\u9662\u7684\u68c0\u6d4b\u62a5\u544a\u4e0a\u68c0\u6d4b\u6280\u672f\u680f\u662f\u5426\u6709\u201c\u8367\u5149\u5b9a\u91cfPCR\u201d\u3001\u201cRT-PCR\u201d\u4ee5\u53ca\u201cARMS\u201d\u7b49\u5b57\u6837\uff0c\u82e5\u6709\u7684\u8bdd\u5219\u4e3aPCR\u6280\u672f\u3002\u5982\u679c\u5404\u4f4d\u4e00\u6b65\u4e00\u6b65\u6392\u67e5\u81ea\u8eab\u68c0\u6d4b\u6d41\u7a0b\uff0c\u786e\u8ba4\u81ea\u8eab\u764c\u79cd\u6709\u9776\u5411\u836f\u4e14\u4f7f\u7528\u4e86\u6b63\u786e\u5408\u683c\u7684\u7ec4\u7ec7\u6837\u672c\uff0c\u6700\u540e\u4f7f\u7528PCR\u6280\u672f\u68c0\u6d4b\u4e3a\u9634\u6027\uff0c\u90a3\u4e48\u6709\u6761\u4ef6\u7684\u5bb6\u5ead\u5728\u5269\u4f59\u6837\u672c\u5145\u8db3\u4ee5\u53ca\u7ecf\u6d4e\u5141\u8bb8\u7684\u60c5\u51b5\u4e0b\uff0c\u53ef\u4ee5\u8003\u8651\u518d\u5c1d\u8bd5NGS\u68c0\u6d4b\uff0c\u770b\u81ea\u8eab\u662f\u5426\u5c5e\u4e8e\u4e0a\u8ff0\u5c0f\u6982\u7387\u7f55\u89c1\u7a81\u53d8\u7c7b\u578b\u3002<\/p>\n<\/div>
\u603b\u7ed3<\/div><\/h4><\/div>\u6700\u540e\u5e2e\u5404\u4f4d\u68b3\u7406\u4e00\u4e0b\u82e5\u57fa\u56e0\u68c0\u6d4b\u62a5\u544a\u9634\u6027\uff0c\u4ece\u57fa\u56e0\u68c0\u6d4b\u89d2\u5ea6\uff0c\u8fd8\u53ef\u4ee5\u505a\u54ea\u4e9b\u5c1d\u8bd5\uff1a<\/p>\n
1. \u56de\u5f52\u75c5\u7406\u62a5\u544a\uff0c\u786e\u8ba4\u81ea\u8eab\u764c\u79cd\uff0c\u4e86\u89e3\u81ea\u8eab\u764c\u79cd\u662f\u5426\u6709\u5bf9\u5e94\u9776\u5411\u836f\u4ee5\u53ca\u9776\u5411\u57fa\u56e0\u7a81\u53d8\u53ef\u80fd\u6027\u5927\u5c0f\uff0c\u82e5\u65e0\u76f8\u5173\u9776\u5411\u836f\uff0c\u5219\u518d\u6b21\u57fa\u56e0\u68c0\u6d4b\u610f\u4e49\u4e0d\u5927\uff1b<\/p>\n
2. \u56de\u987e\u68c0\u6d4b\u4f7f\u7528\u7684\u6837\u672c\u7c7b\u578b\uff0c\u82e5\u4ec5\u4f7f\u7528\u8840\u6db2\u6837\u672c\u4e14\u672a\u68c0\u6d4b\u5230\u4efb\u4f55\u7a81\u53d8\uff0c\u5219\u6709\u6761\u4ef6\u7684\u60c5\u51b5\u4e0b\u53ef\u8003\u8651\u5c3d\u91cf\u83b7\u53d6\u7ec4\u7ec7\u6837\u672c\uff0c\u5728\u786e\u5b9a\u764c\u79cd\u7c7b\u578b\u9002\u5408\u540e\u7528\u7ec4\u7ec7\u6837\u672c\u518d\u884c\u68c0\u6d4b\uff1b<\/p>\n
3. \u56de\u987e\u68c0\u6d4b\u4f7f\u7528\u7684\u6837\u672c\u7c7b\u578b\uff0c\u82e5\u4f7f\u7528\u7684\u4e3a\u65b0\u9c9c\u6807\u672c\u6216\u6837\u672c\u91cf\u4e0d\u8db3\u7684\u77f3\u8721\u5207\u7247\uff0c\u5219\u4ecd\u662f\u56de\u5f52\u75c5\u7406\u62a5\u544a\uff0c\u5148\u786e\u8ba4\u6b64\u6b21\u53d6\u6837\u662f\u5426\u53d6\u5230\u80bf\u7624\u4ee5\u53ca\u80bf\u7624\u542b\u91cf\u662f\u5426\u8fbe\u6807\uff0c\u82e5\u4e0d\u7b26\u5408\u5219\u53ef\u8003\u8651\u91cd\u65b0\u53d6\u6837\uff0c\u8fbe\u6807\u540e\u518d\u9001\u68c0\uff0c\u82e5\u7b26\u5408\u6807\u51c6\u5219\u7ed3\u679c\u57fa\u672c\u53ef\u4fe1\uff0c\u518d\u884c\u68c0\u6d4b\u6539\u53d8\u53ef\u80fd\u6027\u4e0d\u5927\uff1b<\/p>\n
4. \u5ba1\u89c6\u68c0\u6d4b\u6280\u672f\uff0c\u82e5\u764c\u79cd\u3001\u6837\u672c\u5747\u65e0\u95ee\u9898\uff0c\u4f7f\u7528\u7684\u662fPCR\u6280\u672f\u68c0\u6d4b\uff0c\u7ecf\u6d4e\u5141\u8bb8\u4e14\u5269\u4f59\u6837\u672c\u8db3\u591f\u7684\u60c5\u51b5\u4e0b\u53ef\u8003\u8651\u518d\u884cNGS\u6280\u672f\u68c0\u6d4b\uff0c\u4ee5\u514d\u9057\u6f0f\u5c0f\u6982\u7387\u7684\u7f55\u89c1\u7a81\u53d8\u4f4d\u70b9\u53ca\u7c7b\u578b\u3002<\/p>\n
5. \u82e5\u764c\u79cd\u3001\u6837\u672c\uff0c\u8fd8\u6709\u68c0\u6d4b\u6280\u672f\u5747\u65e0\u95ee\u9898\uff0c\u90a3\u4e48\u57fa\u56e0\u68c0\u6d4b\u7ed3\u679c\u95ee\u9898\u4e0d\u5927\uff0c\u57fa\u672c\u4e0d\u4f1a\u6709\u9519\u6f0f\uff0c\u77ed\u671f\u5185\u4e0d\u9700\u8981\u4e8c\u6b21\u68c0\u6d4b\uff0c\u6539\u53d8\u53ef\u80fd\u6027\u4e5f\u4e0d\u9ad8\uff0c\u5b9e\u5728\u60f3\u640f\u4e00\u640f\u5efa\u8bae\u5728\u63a5\u53d7\u6b63\u89c4\u6cbb\u7597\u51fa\u73b0\u8fdb\u5c55\uff0c\u60c5\u51b5\u6709\u53d8\u5316\u4e4b\u540e\u624d\u8003\u8651\u3002<\/p>\n
\u672c\u6587\u7ae0\u8f6c\u8f7d\u4e8e\uff1a \u4e0e\u7231\u5171\u821e\u8ba2\u9605\u53f7<\/p>\n
\u539f\u6587\u94fe\u63a5\uff1ahttps:\/\/mp.weixin.qq.com\/s\/w6yaj-CImt27KZFIDDHHQw<\/p>\n<\/div><\/div><\/div><\/div><\/div><\/p>","protected":false},"excerpt":{"rendered":"
Patients who have just been diagnosed with non-small cell lung cancer have raised a common question: \"What should I do if the first genetic test shows no mutation? Do I need to retest?\" Regarding this issue, I would like to propose the following ideas from the perspective of genetic testing for your reference.<\/p>","protected":false},"author":1,"featured_media":3606,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"content-type":"","om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[202],"tags":[],"class_list":["post-1323","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-202"],"acf":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/posts\/1323","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/comments?post=1323"}],"version-history":[{"count":6,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/posts\/1323\/revisions"}],"predecessor-version":[{"id":3618,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/posts\/1323\/revisions\/3618"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/media\/3606"}],"wp:attachment":[{"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/media?parent=1323"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/categories?post=1323"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/tags?post=1323"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
\u6709\u53e5\u4fd7\u8bed\u8bf4\u201c\u9f99\u751f\u9f99\uff0c\u51e4\u751f\u51e4\u201d\uff0c\u5bd3\u610f\u662f\u4e0d\u540c\u4e8b\u7269\u7684\u7279\u70b9\u6709\u6240\u4e0d\u540c\uff0c\u4e0d\u53ef\u4e00\u6982\u800c\u8bba\uff0c\u80bf\u7624\u6cbb\u7597\u4e5f\u662f\u5982\u6b64\uff0c\u73b0\u9636\u6bb5\u5e76\u975e\u6240\u6709\u764c\u79cd\u90fd\u6709\u53ef\u53ca\u7684\u9776\u5411\u836f\uff0c\u800c\u6709\u5bf9\u5e94\u9776\u5411\u836f\u7684\u764c\u79cd\u7684\u9776\u5411\u57fa\u56e0\u548c\u7a81\u53d8\u6982\u7387\u4e5f\u6709\u6240\u4e0d\u540c\u3002<\/p>\n
\u4f8b\u5982\u4e73\u817a\u764c\u7684\u4e3b\u8981\u9776\u5411\u76f8\u5173\u57fa\u56e0\u4e3aHER2\u3001PIK3CA\u3001BRCA1\/2\uff0c\u9ed1\u8272\u7d20\u7624\u4e3aBRAF\uff0c\u975e\u5c0f\u7ec6\u80de\u80ba\u764c\u5219\u4e3aEGFR\u3001ALK\u3001ROS1\u3001RET\u3001MET\u3001HER2\u3001BRAF\u3001KRAS\u3001NTRK\u7b49\uff0c\u5176\u4e2dBRAF\u57fa\u56e0\u5728\u9ed1\u8272\u7d20\u7624\u4e2d\u7a81\u53d8\u6982\u7387\u8fbe\u523025%\u5de6\u53f3\uff0c\u5728\u975e\u5c0f\u7ec6\u80de\u80ba\u764c\u4e2d\u4ec5\u4e3a1-5%\u5de6\u53f3\u3002<\/p>\n
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<\/span><\/div>\u5f71\u54cd\u56e0\u7d20\u4e8c\uff1a\u68c0\u6d4b\u6837\u672c<\/div><\/h4><\/div>\u660e\u786e\u764c\u79cd\uff0c\u4e86\u89e3\u662f\u5426\u6709\u5bf9\u5e94\u9776\u5411\u836f\uff0c\u77e5\u6653\u57fa\u56e0\u68c0\u6d4b\u53c2\u8003\u4ef7\u503c\u53ea\u662f\u65b9\u5411\uff0c\u65b9\u5411\u5bf9\u4e86\u8fd8\u8981\u770b\u8d70\u7684\u662f\u5426\u8e0f\u5b9e\uff0c\u57fa\u7840\u662f\u5426\u7262\u9760\uff0c\u800c\u57fa\u56e0\u68c0\u6d4b\u7684\u57fa\u7840\u5c31\u662f\u6837\u672c\uff0c\u4e0d\u540c\u7684\u6837\u672c\u7c7b\u578b\u4ece\u6839\u672c\u4e0a\u5f71\u54cd\u4e86\u68c0\u6d4b\u7ed3\u679c\u662f\u5426\u53ef\u9760\u3002<\/p>\n<\/div><\/div><\/div>
\n1. \u7b2c\u4e00\u6b21\u68c0\u6d4b\u4f7f\u7528\u7684\u662f\u5426\u4e3a\u8840\u6db2\u6837\u672c<\/p>\n<\/div><\/h4><\/div>
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2020\u5e74\uff0c\u5929\u6d25\u533b\u79d1\u5927\u5b66\u53d1\u8868\u7814\u7a76\u56de\u987e2018\u5e74\u81f32021\u5e74\u671f\u95f4\u5929\u6d25\u533b\u79d1\u5927\u5b66\u603b\u533b\u9662\u80bf\u7624\u79d1\u6536\u6cbb\u7684NSCLC\u60a3\u8005\uff0c\u5176\u4e2d\u7eb3\u5165\u4e8632\u540d\u65e2\u6709\u7ec4\u7ec7\u6837\u672c\u4e5f\u6709\u8840\u6db2\u6837\u672c\u7684\u60a3\u8005\u5bf9\u6bd4\u4e24\u79cd\u6837\u672c\u7684\u68c0\u6d4b\u7ed3\u679c\uff0c\u572832\u4f8bNSCLC\u60a3\u8005\u4e2d\uff0c\u7ec4\u7ec7\u4e2d\u68c0\u6d4b\u51fa15\u4f8bEGFR\u7a81\u53d8\uff0c\u8840\u6d46\u4e2d\u68c0\u6d4b\u51fa13\u4f8b\u7a81\u53d8\uff08\u5373\u6709\u4e24\u540d\u5b9e\u9645\u6709EGFR\u7a81\u53d8\u7684\u60a3\u8005\u672a\u88ab\u8840\u6db2\u68c0\u6d4b\u51fa\uff09\uff0c\u7a81\u53d8\u4f4d\u70b9\u5b8c\u5168\u4e00\u81f4\u7684\u60a3\u8005\u517110\u4f8b\uff0c5\u4f8b\u60a3\u8005\u7ec4\u7ec7\u4e2d\u68c0\u6d4b\u51fa\u7684EGFR\u7a81\u53d8\u672a\u5728\u8840\u6d46\u4e2d\u68c0\u6d4b\u51fa\uff0c\u7ec4\u7ec7\u4e2d\u672a\u68c0\u6d4b\u5230\u7a81\u53d8\u7684\u8840\u6d46\u4e2d\u5747\u672a\u68c0\u6d4b\u5230\uff0c\u654f\u611f\u6027\u4e3a66.7%\uff0810\/15\uff09\uff0c\u7279\u5f02\u6027\u4e3a100%\uff0817\/17\uff09\uff0c\u4e24\u79cd\u6807\u672c\u68c0\u6d4b\u4e00\u81f4\u6027\u4e3a84.4%\uff0827\/32\uff09[2]\u3002\u7b80\u5355\u6765\u8bf4\uff0c\u8840\u6db2\u4e0d\u4ec5\u6709\u53ef\u80fd\u5c06\u5b9e\u9645\u6709\u7a81\u53d8\u7684\u60a3\u8005\u6f0f\u6389\uff0c\u8fd8\u6709\u53ef\u80fd\u68c0\u6d4b\u5230\u7684\u7a81\u53d8\u4f4d\u70b9\u5c11\u4e8e\u7ec4\u7ec7\u6837\u672c\u3002<\/p>\n
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<\/span><\/div>2. \u7b2c\u4e00\u6b21\u68c0\u6d4b\u4f7f\u7528\u7684\u7ec4\u7ec7\u6837\u672c\u662f\u5426\u7b26\u5408\u8d28\u63a7\u8981\u6c42<\/div><\/h4><\/div>\u672c\u4eba\u4ee5\u524d\u7684\u6587\u7ae0\u4e2d\u4e5f\u63d0\u53ca\u8fc7\u7ec4\u7ec7\u6837\u672c\u7684\u8d28\u91cf\u63a7\u5236\uff0c\u901a\u5e38\u7528\u4e8e\u57fa\u56e0\u68c0\u6d4b\u7684\u7ec4\u7ec7\u6837\u672c\u9700\u8981\u80bf\u7624\u6210\u5206\u542b\u91cf\u536020%\u4ee5\u4e0a\uff0c\u4e00\u822c\u68c0\u6d4b\u516c\u53f8\u9700\u898110-15\u5f20\u4e0d\u67d3\u8272\u7684\u5207\u7247\uff0c\u540c\u65f6\u5bf9\u4e8e\u4ece\u8fd9\u4e9b\u5207\u7247\u4e2d\u63d0\u53d6\u5230\u7684DNA\u603b\u91cf\u4e5f\u6709\u57fa\u672c\u8981\u6c42\uff0c\u5927\u81f4\u5982\u4e0b\u3002<\/p>\n<\/div>
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\u7ec4\u7ec7\u6837\u672c\u5e76\u975e\u4e07\u65e0\u4e00\u5931\uff0c\u82e5\u4f7f\u7528\u7ec4\u7ec7\u6837\u672c\u68c0\u6d4b\u4e5f\u4e3a\u9634\u6027\uff0c\u9700\u5ba1\u89c6\u4e00\u4e0b\u81ea\u8eab\u662f\u5426\u72af\u4e86\u4e0a\u8ff0\u7126\u6025\u5f15\u8d77\u7684\u9519\u8bef\uff0c\u4e07\u4e00\u7b26\u5408\u5219\u53ef\u8003\u8651\u91cd\u65b0\u53d6\u6837\u6216\u8005\u7b49\u75c5\u7406\u8bca\u65ad\u51fa\u6765\u4e4b\u540e\u518d\u6b21\u68c0\u6d4b\u3002<\/p>\n
\uff08\u6ce8\u610f\uff1a\u901a\u5e38\u75c5\u7406\u62a5\u544a3-5\u4e2a\u5de5\u4f5c\u65e5\u5c31\u6709\u7ed3\u679c\uff0c\u9664\u4e86\u786e\u8bca\u764c\u79cd\u4ee5\u5916\uff0c\u8fd8\u53ef\u4ee5\u5927\u81f4\u4e86\u89e3\u6837\u672c\u4e2d\u80bf\u7624\u542b\u91cf\u591a\u5c11\uff0c\u4e0d\u4f1a\u803d\u8bef\u5404\u4f4d\u592a\u591a\u65f6\u95f4\u53cd\u800c\u8fd8\u80fd\u5e2e\u52a9\u4e86\u89e3\u6837\u672c\u8d28\u91cf\u597d\u574f\uff0c\u5c3d\u91cf\u4e0d\u8981\u7701\u7565\uff09<\/p>\n<\/div>
<\/span><\/div><\/div><\/div>\u5f71\u54cd\u56e0\u7d20\u4e09\uff1a\u68c0\u6d4b\u6280\u672f<\/div><\/h4><\/div>\u5f88\u591a\u5584\u4e8e\u5b66\u4e60\u7684\u75c5\u53cb\u5bf9\u4e8e\u68c0\u6d4b\u6280\u672f\u4e5f\u6709\u6240\u4e86\u89e3\uff0c\u5bf9\u4e8ePCR\u548cNGS\u6280\u672f\u5e76\u4e0d\u964c\u751f\uff0c\u6211\u4e4b\u524d\u4e5f\u5199\u8fc7\u300a\u9662\u5185\u9662\u5916\u57fa\u56e0\u68c0\u6d4b\u7684\u533a\u522b\u300b\uff08\u70b9\u51fb\u9605\u8bfb\uff09\uff0c\u5927\u90e8\u5206\u533b\u9662\u9662\u5185\u5747\u91c7\u7528PCR\u6280\u672f\uff0c\u65b9\u4fbf\u5feb\u6377\u4e14\u57fa\u672c\u80fd\u591f\u8986\u76d6\u9664NTRK\u4ee5\u5916\u7684\u5176\u4ed6\u9776\u5411\u57fa\u56e0\uff0c\u53ef\u4ee5\u6ee1\u8db3\u5927\u591a\u6570\u60a3\u8005\u7684\u68c0\u6d4b\u9700\u6c42\uff0c\u4e0d\u8fc7PCR\u6280\u672f\u5b58\u5728\u4e00\u5b9a\u7f3a\u9677\uff0c\u53ef\u80fd\u9020\u6210\u6781\u5c0f\u6982\u7387\u9057\u6f0f\u3002<\/p>\n<\/div>
\n1.PCR\u6280\u672f\u96be\u4ee5\u8986\u76d6\u9776\u5411\u57fa\u56e0\u7684\u7f55\u89c1\u7a81\u53d8\u578b<\/p>\n<\/div><\/h4><\/div>
\u901a\u4fd7\u4e00\u70b9\u8bf4\uff0cPCR\u6280\u672f\u5c31\u50cf\u662f\u62ff\u7740\u73b0\u6210\u7684\u6863\u6848\u53bb\u6392\u67e5\u5acc\u7591\u4eba\uff0c\u800cNGS\u6280\u672f\u5219\u662f\u5730\u6bef\u5f0f\u641c\u67e5\uff0c\u65e0\u8bba\u662f\u6863\u6848\u91cc\u7684\u8fd8\u662f\u6863\u6848\u5916\u7684\uff0c\u53ea\u8981\u68c0\u67e5\u53d1\u73b0\u6709\u95ee\u9898\u7684\u901a\u901a\u4e0a\u62a5\u3002PCR\u6280\u672f\u53ef\u7528\u4e8e\u68c0\u6d4b\u70b9\u7a81\u53d8\u4e0e\u878d\u5408\u7a81\u53d8\uff0c\u53ef\u8986\u76d6\u76ee\u6807\u57fa\u56e0\u7684\u5404\u79cd\u5df2\u77e5\u7a81\u53d8\u7c7b\u578b\uff0c\u4f46\u5bf9\u4e8e\u672a\u77e5\u53ca\u7f55\u89c1\u7a81\u53d8\u4f4d\u70b9\u5219\u7565\u663e\u65e0\u529b\u3002<\/p>\n
\u4ee5\u6700\u5e38\u89c1\u7684EGFR\u57fa\u56e0\u68c0\u6d4b\u4e3a\u4f8b\uff0c\u76ee\u524d\u56fd\u5185\u5df2\u83b7\u6279\u7684PCR\u8bd5\u5242\u76d2\u7684\u68c0\u6d4b\u4f4d\u70b9\u4e3b\u8981\u5305\u62ec\u5404\u7c7bEGFR 19\u7f3a\u5931\u7a81\u53d8\u3001EGFR L858R\u7a81\u53d8\u3001EGFR T790M\u7a81\u53d8\uff0cEGFR 20\u63d2\u5165\u4ee5\u53ca\u90e8\u5206\u4f4d\u70b9\u3002<\/p>\n
\u867d\u7136\u8fd9\u4e9b\u5df2\u7ecf\u8986\u76d6\u4e86\u8d85\u8fc790%\u7684EGFR\u7a81\u53d8\uff0c\u4f46EGFR\u57fa\u56e0\u768418\u300119\u300120\u300121\u5916\u663e\u5b50\u5185\u4ecd\u53ef\u80fd\u51fa\u73b0\u5982EGFR C797S\u3001EGFR L833V\u3001EGFR L747Q\u7b49\u7b49\u66f4\u52a0\u5c11\u89c1\u7684\u7a81\u53d8\u4f4d\u70b9\u3002\u8fd9\u4e9b\u7a81\u53d8\u6216\u51fa\u73b0\u7684\u6982\u7387\u6781\u4f4e\uff08\u5373\u7f55\u89c1\u7a81\u53d8\u4f4d\u70b9\uff09\u6216\u7279\u6b8a\u60c5\u51b5\u4e0b\u53ef\u80fd\u51fa\u73b0\uff0c\u53ef\u80fd\u4ec5\u5360\u6240\u6709EGFR\u7a81\u53d8\u76841-5%\u5de6\u53f3\uff08\u8f83\u96be\u6709\u5b8c\u5168\u7edf\u8ba1\uff09\uff0c\u5bf9\u4e8e\u8fd9\u4e9b\u4e0d\u5728\u68c0\u6d4b\u8303\u56f4\u5185\u7684\u7a81\u53d8\u4f4d\u70b9\uff0cPCR\u6280\u672f\u65e0\u80fd\u4e3a\u529b\uff0c\u4f46NGS\u6280\u672f\u5219\u6709\u53ef\u80fd\u68c0\u51fa\u3002<\/p>\n<\/div>
<\/span><\/div>\u518d\u4ee5\u88ab\u79f0\u4e3a\u201c\u94bb\u77f3\u7a81\u53d8\u201d\u7684ALK\u878d\u5408\u7a81\u53d8\u4e3a\u4f8b\uff0c\u8be5\u7a81\u53d8\u7c7b\u578b\u4e0d\u540c\u4e8e\u4e0a\u8ff0\u7684EGFR\u70b9\u7a81\u53d8\uff0c\u5e76\u4e0d\u662f\u5355\u4e2a\u57fa\u56e0\u7684\u4e8b\uff0c\u5b83\u662f\u4e24\u4e2a\u57fa\u56e0\u95f4\u7684\u65ad\u88c2\u4e0e\u878d\u5408\uff0c\u6211\u4eec\u5c06\u8fd9\u79cd\u878d\u5408\u7684\u4e24\u4e2a\u57fa\u56e0\u6210\u4e3a\u201c\u878d\u5408\u4f34\u4fa3\u201d\uff0cALK\u878d\u5408\u5373\u662f\u6307\u5176\u4e2d\u4e00\u4e2a\u4f34\u4fa3\u56fa\u5b9a\u4e3aALK\u57fa\u56e0\uff0c\u53e6\u4e00\u534a\u53ef\u80fd\u4f1a\u6709\u591a\u79cd\u3002<\/p>\n
\u6700\u5e38\u89c1\u7684ALK\u878d\u5408\u53d1\u751f\u5728EML4\u4e0eALK\u57fa\u56e0\u4e4b\u95f4\uff0c\u4f46\u968f\u7740\u7814\u7a76\u53d1\u5c55\uff0c\u6211\u4eec\u53d1\u73b0\u4e86\u66f4\u591a\u4f34\u4fa3\u57fa\u56e0\uff0c\u8f83\u65e9\u4ee5\u524d\u7684PCR\u8bd5\u5242\u76d2\u4ec5\u53ef\u68c0\u6d4bEML4-ALK\u878d\u5408\u7c7b\u578b\uff0c\u5176\u4ed6\u878d\u5408\u4f34\u4fa3\u5f62\u6210\u7684ALK\u878d\u5408\u5219\u65e0\u6cd5\u53d1\u73b0\uff0c\u73b0\u5728\u6709\u4e9b\u66f4\u65b0\u7684PCR\u8bd5\u5242\u76d2\u9664EML4\u57fa\u56e0\u4ee5\u5916\uff0c\u8fd8\u5305\u542bKIF5B\u3001KCL1\u3001TFG\u7b49\u6982\u7387\u76f8\u5bf9\u8f83\u4f4e\u7684\u878d\u5408\u4f34\u4fa3\uff0c\u8fdb\u4e00\u6b65\u6269\u5927\u4e86PCR\u6280\u672f\u53ef\u68c0\u6d4b\u5230\u7684ALK\u878d\u5408\u7c7b\u578b\u3002<\/p>\n
\u53ef\u4ee5\u8bf490%\u751a\u81f395%\u4ee5\u4e0a\u7684ALK\u878d\u5408\u60a3\u8005\u90fd\u53ef\u901a\u8fc7PCR\u6280\u672f\u53d1\u73b0\uff0c\u4f46ALK\u57fa\u56e0\u7684\u878d\u5408\u4f34\u4fa3\u4ecd\u7136\u8d85\u51fa\u6b64\u8303\u56f4\uff0c\u4f8b\u5982\u6211\u66fe\u9047\u5230\u8fc7\u67d0\u4e2a\u75c5\u53cb\u662fDIRC3-ALK\u878d\u5408\uff0cNGS\u6280\u672f\u7684\u7279\u6027\u8ba9\u5b83\u4e0d\u53d7\u8fd9\u79cd\u8303\u56f4\u9650\u5236\uff0c\u65e0\u8bba\u54ea\u79cd\u878d\u5408\u4f34\u4fa3\uff0c\u7406\u8bba\u4e0a\u90fd\u53ef\u4ee5\u68c0\u51fa\uff0c\u907f\u514d\u8fd9\u79cd\u5c0f\u6982\u7387\u610f\u5916\u3002<\/p>\n<\/div>
<\/span><\/div>\n2. PCR\u6280\u672f\u80fd\u4ee5\u8986\u76d6\u90e8\u5206\u7a81\u53d8\u7c7b\u578b<\/p>\n<\/div><\/h4><\/div>
PCR\u6280\u672f\u5bf9\u4e8e\u68c0\u6d4b\u70b9\u7a81\u53d8\u3001\u878d\u5408\u7a81\u53d8\u7684\u68c0\u6d4b\u80fd\u529b\u8f83\u5f3a\uff0c\u4f46\u76ee\u524d\u5404\u533b\u9662\u5e38\u7528\u7684PCR\u68c0\u6d4b\u8bd5\u5242\u76d2\u5bf9\u4e8e\u201c\u6269\u589e\u201d\u8fd9\u4e00\u7a81\u53d8\u7c7b\u578b\u57fa\u672c\u6ca1\u6709\u8986\u76d6\uff0c\u800c\u643a\u5e26HER2\u6269\u589e\u6216\u8005MET\u6269\u589e\u7684\u975e\u5c0f\u7ec6\u80de\u80ba\u764c\u60a3\u8005\u5206\u522b\u5360\u5230\u4e86\u6574\u4f53\u4eba\u7fa4\u76841-3%\uff0c\u82e5\u4f7f\u7528PCR\u6280\u672f\u53ef\u80fd\u5bfc\u81f4\u8fd9\u4e9b\u53ef\u7528\u836f\u7684\u9a71\u52a8\u57fa\u56e0\u7a81\u53d8\u7684\u60a3\u8005\u4f1a\u88ab\u9057\u6f0f\u3002<\/p>\n<\/div>
<\/span><\/div><\/div><\/div>\u5404\u4f4d\u53ef\u901a\u8fc7\u68c0\u67e5\u81ea\u5df1\u62ff\u5230\u7684\u533b\u9662\u7684\u68c0\u6d4b\u62a5\u544a\u4e0a\u68c0\u6d4b\u6280\u672f\u680f\u662f\u5426\u6709\u201c\u8367\u5149\u5b9a\u91cfPCR\u201d\u3001\u201cRT-PCR\u201d\u4ee5\u53ca\u201cARMS\u201d\u7b49\u5b57\u6837\uff0c\u82e5\u6709\u7684\u8bdd\u5219\u4e3aPCR\u6280\u672f\u3002\u5982\u679c\u5404\u4f4d\u4e00\u6b65\u4e00\u6b65\u6392\u67e5\u81ea\u8eab\u68c0\u6d4b\u6d41\u7a0b\uff0c\u786e\u8ba4\u81ea\u8eab\u764c\u79cd\u6709\u9776\u5411\u836f\u4e14\u4f7f\u7528\u4e86\u6b63\u786e\u5408\u683c\u7684\u7ec4\u7ec7\u6837\u672c\uff0c\u6700\u540e\u4f7f\u7528PCR\u6280\u672f\u68c0\u6d4b\u4e3a\u9634\u6027\uff0c\u90a3\u4e48\u6709\u6761\u4ef6\u7684\u5bb6\u5ead\u5728\u5269\u4f59\u6837\u672c\u5145\u8db3\u4ee5\u53ca\u7ecf\u6d4e\u5141\u8bb8\u7684\u60c5\u51b5\u4e0b\uff0c\u53ef\u4ee5\u8003\u8651\u518d\u5c1d\u8bd5NGS\u68c0\u6d4b\uff0c\u770b\u81ea\u8eab\u662f\u5426\u5c5e\u4e8e\u4e0a\u8ff0\u5c0f\u6982\u7387\u7f55\u89c1\u7a81\u53d8\u7c7b\u578b\u3002<\/p>\n<\/div>
\u603b\u7ed3<\/div><\/h4><\/div>\u6700\u540e\u5e2e\u5404\u4f4d\u68b3\u7406\u4e00\u4e0b\u82e5\u57fa\u56e0\u68c0\u6d4b\u62a5\u544a\u9634\u6027\uff0c\u4ece\u57fa\u56e0\u68c0\u6d4b\u89d2\u5ea6\uff0c\u8fd8\u53ef\u4ee5\u505a\u54ea\u4e9b\u5c1d\u8bd5\uff1a<\/p>\n
1. \u56de\u5f52\u75c5\u7406\u62a5\u544a\uff0c\u786e\u8ba4\u81ea\u8eab\u764c\u79cd\uff0c\u4e86\u89e3\u81ea\u8eab\u764c\u79cd\u662f\u5426\u6709\u5bf9\u5e94\u9776\u5411\u836f\u4ee5\u53ca\u9776\u5411\u57fa\u56e0\u7a81\u53d8\u53ef\u80fd\u6027\u5927\u5c0f\uff0c\u82e5\u65e0\u76f8\u5173\u9776\u5411\u836f\uff0c\u5219\u518d\u6b21\u57fa\u56e0\u68c0\u6d4b\u610f\u4e49\u4e0d\u5927\uff1b<\/p>\n
2. \u56de\u987e\u68c0\u6d4b\u4f7f\u7528\u7684\u6837\u672c\u7c7b\u578b\uff0c\u82e5\u4ec5\u4f7f\u7528\u8840\u6db2\u6837\u672c\u4e14\u672a\u68c0\u6d4b\u5230\u4efb\u4f55\u7a81\u53d8\uff0c\u5219\u6709\u6761\u4ef6\u7684\u60c5\u51b5\u4e0b\u53ef\u8003\u8651\u5c3d\u91cf\u83b7\u53d6\u7ec4\u7ec7\u6837\u672c\uff0c\u5728\u786e\u5b9a\u764c\u79cd\u7c7b\u578b\u9002\u5408\u540e\u7528\u7ec4\u7ec7\u6837\u672c\u518d\u884c\u68c0\u6d4b\uff1b<\/p>\n
3. \u56de\u987e\u68c0\u6d4b\u4f7f\u7528\u7684\u6837\u672c\u7c7b\u578b\uff0c\u82e5\u4f7f\u7528\u7684\u4e3a\u65b0\u9c9c\u6807\u672c\u6216\u6837\u672c\u91cf\u4e0d\u8db3\u7684\u77f3\u8721\u5207\u7247\uff0c\u5219\u4ecd\u662f\u56de\u5f52\u75c5\u7406\u62a5\u544a\uff0c\u5148\u786e\u8ba4\u6b64\u6b21\u53d6\u6837\u662f\u5426\u53d6\u5230\u80bf\u7624\u4ee5\u53ca\u80bf\u7624\u542b\u91cf\u662f\u5426\u8fbe\u6807\uff0c\u82e5\u4e0d\u7b26\u5408\u5219\u53ef\u8003\u8651\u91cd\u65b0\u53d6\u6837\uff0c\u8fbe\u6807\u540e\u518d\u9001\u68c0\uff0c\u82e5\u7b26\u5408\u6807\u51c6\u5219\u7ed3\u679c\u57fa\u672c\u53ef\u4fe1\uff0c\u518d\u884c\u68c0\u6d4b\u6539\u53d8\u53ef\u80fd\u6027\u4e0d\u5927\uff1b<\/p>\n
4. \u5ba1\u89c6\u68c0\u6d4b\u6280\u672f\uff0c\u82e5\u764c\u79cd\u3001\u6837\u672c\u5747\u65e0\u95ee\u9898\uff0c\u4f7f\u7528\u7684\u662fPCR\u6280\u672f\u68c0\u6d4b\uff0c\u7ecf\u6d4e\u5141\u8bb8\u4e14\u5269\u4f59\u6837\u672c\u8db3\u591f\u7684\u60c5\u51b5\u4e0b\u53ef\u8003\u8651\u518d\u884cNGS\u6280\u672f\u68c0\u6d4b\uff0c\u4ee5\u514d\u9057\u6f0f\u5c0f\u6982\u7387\u7684\u7f55\u89c1\u7a81\u53d8\u4f4d\u70b9\u53ca\u7c7b\u578b\u3002<\/p>\n
5. \u82e5\u764c\u79cd\u3001\u6837\u672c\uff0c\u8fd8\u6709\u68c0\u6d4b\u6280\u672f\u5747\u65e0\u95ee\u9898\uff0c\u90a3\u4e48\u57fa\u56e0\u68c0\u6d4b\u7ed3\u679c\u95ee\u9898\u4e0d\u5927\uff0c\u57fa\u672c\u4e0d\u4f1a\u6709\u9519\u6f0f\uff0c\u77ed\u671f\u5185\u4e0d\u9700\u8981\u4e8c\u6b21\u68c0\u6d4b\uff0c\u6539\u53d8\u53ef\u80fd\u6027\u4e5f\u4e0d\u9ad8\uff0c\u5b9e\u5728\u60f3\u640f\u4e00\u640f\u5efa\u8bae\u5728\u63a5\u53d7\u6b63\u89c4\u6cbb\u7597\u51fa\u73b0\u8fdb\u5c55\uff0c\u60c5\u51b5\u6709\u53d8\u5316\u4e4b\u540e\u624d\u8003\u8651\u3002<\/p>\n
\u672c\u6587\u7ae0\u8f6c\u8f7d\u4e8e\uff1a \u4e0e\u7231\u5171\u821e\u8ba2\u9605\u53f7<\/p>\n
\u539f\u6587\u94fe\u63a5\uff1ahttps:\/\/mp.weixin.qq.com\/s\/w6yaj-CImt27KZFIDDHHQw<\/p>\n<\/div><\/div><\/div><\/div><\/div><\/p>","protected":false},"excerpt":{"rendered":"
Patients who have just been diagnosed with non-small cell lung cancer have raised a common question: \"What should I do if the first genetic test shows no mutation? Do I need to retest?\" Regarding this issue, I would like to propose the following ideas from the perspective of genetic testing for your reference.<\/p>","protected":false},"author":1,"featured_media":3606,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"content-type":"","om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[202],"tags":[],"class_list":["post-1323","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-202"],"acf":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/posts\/1323","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/comments?post=1323"}],"version-history":[{"count":6,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/posts\/1323\/revisions"}],"predecessor-version":[{"id":3618,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/posts\/1323\/revisions\/3618"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/media\/3606"}],"wp:attachment":[{"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/media?parent=1323"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/categories?post=1323"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/tags?post=1323"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
\u660e\u786e\u764c\u79cd\uff0c\u4e86\u89e3\u662f\u5426\u6709\u5bf9\u5e94\u9776\u5411\u836f\uff0c\u77e5\u6653\u57fa\u56e0\u68c0\u6d4b\u53c2\u8003\u4ef7\u503c\u53ea\u662f\u65b9\u5411\uff0c\u65b9\u5411\u5bf9\u4e86\u8fd8\u8981\u770b\u8d70\u7684\u662f\u5426\u8e0f\u5b9e\uff0c\u57fa\u7840\u662f\u5426\u7262\u9760\uff0c\u800c\u57fa\u56e0\u68c0\u6d4b\u7684\u57fa\u7840\u5c31\u662f\u6837\u672c\uff0c\u4e0d\u540c\u7684\u6837\u672c\u7c7b\u578b\u4ece\u6839\u672c\u4e0a\u5f71\u54cd\u4e86\u68c0\u6d4b\u7ed3\u679c\u662f\u5426\u53ef\u9760\u3002<\/p>\n<\/div><\/div><\/div>
\n1. \u7b2c\u4e00\u6b21\u68c0\u6d4b\u4f7f\u7528\u7684\u662f\u5426\u4e3a\u8840\u6db2\u6837\u672c<\/p>\n<\/div><\/h4><\/div>
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2020\u5e74\uff0c\u5929\u6d25\u533b\u79d1\u5927\u5b66\u53d1\u8868\u7814\u7a76\u56de\u987e2018\u5e74\u81f32021\u5e74\u671f\u95f4\u5929\u6d25\u533b\u79d1\u5927\u5b66\u603b\u533b\u9662\u80bf\u7624\u79d1\u6536\u6cbb\u7684NSCLC\u60a3\u8005\uff0c\u5176\u4e2d\u7eb3\u5165\u4e8632\u540d\u65e2\u6709\u7ec4\u7ec7\u6837\u672c\u4e5f\u6709\u8840\u6db2\u6837\u672c\u7684\u60a3\u8005\u5bf9\u6bd4\u4e24\u79cd\u6837\u672c\u7684\u68c0\u6d4b\u7ed3\u679c\uff0c\u572832\u4f8bNSCLC\u60a3\u8005\u4e2d\uff0c\u7ec4\u7ec7\u4e2d\u68c0\u6d4b\u51fa15\u4f8bEGFR\u7a81\u53d8\uff0c\u8840\u6d46\u4e2d\u68c0\u6d4b\u51fa13\u4f8b\u7a81\u53d8\uff08\u5373\u6709\u4e24\u540d\u5b9e\u9645\u6709EGFR\u7a81\u53d8\u7684\u60a3\u8005\u672a\u88ab\u8840\u6db2\u68c0\u6d4b\u51fa\uff09\uff0c\u7a81\u53d8\u4f4d\u70b9\u5b8c\u5168\u4e00\u81f4\u7684\u60a3\u8005\u517110\u4f8b\uff0c5\u4f8b\u60a3\u8005\u7ec4\u7ec7\u4e2d\u68c0\u6d4b\u51fa\u7684EGFR\u7a81\u53d8\u672a\u5728\u8840\u6d46\u4e2d\u68c0\u6d4b\u51fa\uff0c\u7ec4\u7ec7\u4e2d\u672a\u68c0\u6d4b\u5230\u7a81\u53d8\u7684\u8840\u6d46\u4e2d\u5747\u672a\u68c0\u6d4b\u5230\uff0c\u654f\u611f\u6027\u4e3a66.7%\uff0810\/15\uff09\uff0c\u7279\u5f02\u6027\u4e3a100%\uff0817\/17\uff09\uff0c\u4e24\u79cd\u6807\u672c\u68c0\u6d4b\u4e00\u81f4\u6027\u4e3a84.4%\uff0827\/32\uff09[2]\u3002\u7b80\u5355\u6765\u8bf4\uff0c\u8840\u6db2\u4e0d\u4ec5\u6709\u53ef\u80fd\u5c06\u5b9e\u9645\u6709\u7a81\u53d8\u7684\u60a3\u8005\u6f0f\u6389\uff0c\u8fd8\u6709\u53ef\u80fd\u68c0\u6d4b\u5230\u7684\u7a81\u53d8\u4f4d\u70b9\u5c11\u4e8e\u7ec4\u7ec7\u6837\u672c\u3002<\/p>\n
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<\/span><\/div>2. \u7b2c\u4e00\u6b21\u68c0\u6d4b\u4f7f\u7528\u7684\u7ec4\u7ec7\u6837\u672c\u662f\u5426\u7b26\u5408\u8d28\u63a7\u8981\u6c42<\/div><\/h4><\/div>\u672c\u4eba\u4ee5\u524d\u7684\u6587\u7ae0\u4e2d\u4e5f\u63d0\u53ca\u8fc7\u7ec4\u7ec7\u6837\u672c\u7684\u8d28\u91cf\u63a7\u5236\uff0c\u901a\u5e38\u7528\u4e8e\u57fa\u56e0\u68c0\u6d4b\u7684\u7ec4\u7ec7\u6837\u672c\u9700\u8981\u80bf\u7624\u6210\u5206\u542b\u91cf\u536020%\u4ee5\u4e0a\uff0c\u4e00\u822c\u68c0\u6d4b\u516c\u53f8\u9700\u898110-15\u5f20\u4e0d\u67d3\u8272\u7684\u5207\u7247\uff0c\u540c\u65f6\u5bf9\u4e8e\u4ece\u8fd9\u4e9b\u5207\u7247\u4e2d\u63d0\u53d6\u5230\u7684DNA\u603b\u91cf\u4e5f\u6709\u57fa\u672c\u8981\u6c42\uff0c\u5927\u81f4\u5982\u4e0b\u3002<\/p>\n<\/div>
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<\/span><\/div><\/div><\/div>\u5f71\u54cd\u56e0\u7d20\u4e09\uff1a\u68c0\u6d4b\u6280\u672f<\/div><\/h4><\/div>\u5f88\u591a\u5584\u4e8e\u5b66\u4e60\u7684\u75c5\u53cb\u5bf9\u4e8e\u68c0\u6d4b\u6280\u672f\u4e5f\u6709\u6240\u4e86\u89e3\uff0c\u5bf9\u4e8ePCR\u548cNGS\u6280\u672f\u5e76\u4e0d\u964c\u751f\uff0c\u6211\u4e4b\u524d\u4e5f\u5199\u8fc7\u300a\u9662\u5185\u9662\u5916\u57fa\u56e0\u68c0\u6d4b\u7684\u533a\u522b\u300b\uff08\u70b9\u51fb\u9605\u8bfb\uff09\uff0c\u5927\u90e8\u5206\u533b\u9662\u9662\u5185\u5747\u91c7\u7528PCR\u6280\u672f\uff0c\u65b9\u4fbf\u5feb\u6377\u4e14\u57fa\u672c\u80fd\u591f\u8986\u76d6\u9664NTRK\u4ee5\u5916\u7684\u5176\u4ed6\u9776\u5411\u57fa\u56e0\uff0c\u53ef\u4ee5\u6ee1\u8db3\u5927\u591a\u6570\u60a3\u8005\u7684\u68c0\u6d4b\u9700\u6c42\uff0c\u4e0d\u8fc7PCR\u6280\u672f\u5b58\u5728\u4e00\u5b9a\u7f3a\u9677\uff0c\u53ef\u80fd\u9020\u6210\u6781\u5c0f\u6982\u7387\u9057\u6f0f\u3002<\/p>\n<\/div>
\n1.PCR\u6280\u672f\u96be\u4ee5\u8986\u76d6\u9776\u5411\u57fa\u56e0\u7684\u7f55\u89c1\u7a81\u53d8\u578b<\/p>\n<\/div><\/h4><\/div>
\u901a\u4fd7\u4e00\u70b9\u8bf4\uff0cPCR\u6280\u672f\u5c31\u50cf\u662f\u62ff\u7740\u73b0\u6210\u7684\u6863\u6848\u53bb\u6392\u67e5\u5acc\u7591\u4eba\uff0c\u800cNGS\u6280\u672f\u5219\u662f\u5730\u6bef\u5f0f\u641c\u67e5\uff0c\u65e0\u8bba\u662f\u6863\u6848\u91cc\u7684\u8fd8\u662f\u6863\u6848\u5916\u7684\uff0c\u53ea\u8981\u68c0\u67e5\u53d1\u73b0\u6709\u95ee\u9898\u7684\u901a\u901a\u4e0a\u62a5\u3002PCR\u6280\u672f\u53ef\u7528\u4e8e\u68c0\u6d4b\u70b9\u7a81\u53d8\u4e0e\u878d\u5408\u7a81\u53d8\uff0c\u53ef\u8986\u76d6\u76ee\u6807\u57fa\u56e0\u7684\u5404\u79cd\u5df2\u77e5\u7a81\u53d8\u7c7b\u578b\uff0c\u4f46\u5bf9\u4e8e\u672a\u77e5\u53ca\u7f55\u89c1\u7a81\u53d8\u4f4d\u70b9\u5219\u7565\u663e\u65e0\u529b\u3002<\/p>\n
\u4ee5\u6700\u5e38\u89c1\u7684EGFR\u57fa\u56e0\u68c0\u6d4b\u4e3a\u4f8b\uff0c\u76ee\u524d\u56fd\u5185\u5df2\u83b7\u6279\u7684PCR\u8bd5\u5242\u76d2\u7684\u68c0\u6d4b\u4f4d\u70b9\u4e3b\u8981\u5305\u62ec\u5404\u7c7bEGFR 19\u7f3a\u5931\u7a81\u53d8\u3001EGFR L858R\u7a81\u53d8\u3001EGFR T790M\u7a81\u53d8\uff0cEGFR 20\u63d2\u5165\u4ee5\u53ca\u90e8\u5206\u4f4d\u70b9\u3002<\/p>\n
\u867d\u7136\u8fd9\u4e9b\u5df2\u7ecf\u8986\u76d6\u4e86\u8d85\u8fc790%\u7684EGFR\u7a81\u53d8\uff0c\u4f46EGFR\u57fa\u56e0\u768418\u300119\u300120\u300121\u5916\u663e\u5b50\u5185\u4ecd\u53ef\u80fd\u51fa\u73b0\u5982EGFR C797S\u3001EGFR L833V\u3001EGFR L747Q\u7b49\u7b49\u66f4\u52a0\u5c11\u89c1\u7684\u7a81\u53d8\u4f4d\u70b9\u3002\u8fd9\u4e9b\u7a81\u53d8\u6216\u51fa\u73b0\u7684\u6982\u7387\u6781\u4f4e\uff08\u5373\u7f55\u89c1\u7a81\u53d8\u4f4d\u70b9\uff09\u6216\u7279\u6b8a\u60c5\u51b5\u4e0b\u53ef\u80fd\u51fa\u73b0\uff0c\u53ef\u80fd\u4ec5\u5360\u6240\u6709EGFR\u7a81\u53d8\u76841-5%\u5de6\u53f3\uff08\u8f83\u96be\u6709\u5b8c\u5168\u7edf\u8ba1\uff09\uff0c\u5bf9\u4e8e\u8fd9\u4e9b\u4e0d\u5728\u68c0\u6d4b\u8303\u56f4\u5185\u7684\u7a81\u53d8\u4f4d\u70b9\uff0cPCR\u6280\u672f\u65e0\u80fd\u4e3a\u529b\uff0c\u4f46NGS\u6280\u672f\u5219\u6709\u53ef\u80fd\u68c0\u51fa\u3002<\/p>\n<\/div>
<\/span><\/div>\u518d\u4ee5\u88ab\u79f0\u4e3a\u201c\u94bb\u77f3\u7a81\u53d8\u201d\u7684ALK\u878d\u5408\u7a81\u53d8\u4e3a\u4f8b\uff0c\u8be5\u7a81\u53d8\u7c7b\u578b\u4e0d\u540c\u4e8e\u4e0a\u8ff0\u7684EGFR\u70b9\u7a81\u53d8\uff0c\u5e76\u4e0d\u662f\u5355\u4e2a\u57fa\u56e0\u7684\u4e8b\uff0c\u5b83\u662f\u4e24\u4e2a\u57fa\u56e0\u95f4\u7684\u65ad\u88c2\u4e0e\u878d\u5408\uff0c\u6211\u4eec\u5c06\u8fd9\u79cd\u878d\u5408\u7684\u4e24\u4e2a\u57fa\u56e0\u6210\u4e3a\u201c\u878d\u5408\u4f34\u4fa3\u201d\uff0cALK\u878d\u5408\u5373\u662f\u6307\u5176\u4e2d\u4e00\u4e2a\u4f34\u4fa3\u56fa\u5b9a\u4e3aALK\u57fa\u56e0\uff0c\u53e6\u4e00\u534a\u53ef\u80fd\u4f1a\u6709\u591a\u79cd\u3002<\/p>\n
\u6700\u5e38\u89c1\u7684ALK\u878d\u5408\u53d1\u751f\u5728EML4\u4e0eALK\u57fa\u56e0\u4e4b\u95f4\uff0c\u4f46\u968f\u7740\u7814\u7a76\u53d1\u5c55\uff0c\u6211\u4eec\u53d1\u73b0\u4e86\u66f4\u591a\u4f34\u4fa3\u57fa\u56e0\uff0c\u8f83\u65e9\u4ee5\u524d\u7684PCR\u8bd5\u5242\u76d2\u4ec5\u53ef\u68c0\u6d4bEML4-ALK\u878d\u5408\u7c7b\u578b\uff0c\u5176\u4ed6\u878d\u5408\u4f34\u4fa3\u5f62\u6210\u7684ALK\u878d\u5408\u5219\u65e0\u6cd5\u53d1\u73b0\uff0c\u73b0\u5728\u6709\u4e9b\u66f4\u65b0\u7684PCR\u8bd5\u5242\u76d2\u9664EML4\u57fa\u56e0\u4ee5\u5916\uff0c\u8fd8\u5305\u542bKIF5B\u3001KCL1\u3001TFG\u7b49\u6982\u7387\u76f8\u5bf9\u8f83\u4f4e\u7684\u878d\u5408\u4f34\u4fa3\uff0c\u8fdb\u4e00\u6b65\u6269\u5927\u4e86PCR\u6280\u672f\u53ef\u68c0\u6d4b\u5230\u7684ALK\u878d\u5408\u7c7b\u578b\u3002<\/p>\n
\u53ef\u4ee5\u8bf490%\u751a\u81f395%\u4ee5\u4e0a\u7684ALK\u878d\u5408\u60a3\u8005\u90fd\u53ef\u901a\u8fc7PCR\u6280\u672f\u53d1\u73b0\uff0c\u4f46ALK\u57fa\u56e0\u7684\u878d\u5408\u4f34\u4fa3\u4ecd\u7136\u8d85\u51fa\u6b64\u8303\u56f4\uff0c\u4f8b\u5982\u6211\u66fe\u9047\u5230\u8fc7\u67d0\u4e2a\u75c5\u53cb\u662fDIRC3-ALK\u878d\u5408\uff0cNGS\u6280\u672f\u7684\u7279\u6027\u8ba9\u5b83\u4e0d\u53d7\u8fd9\u79cd\u8303\u56f4\u9650\u5236\uff0c\u65e0\u8bba\u54ea\u79cd\u878d\u5408\u4f34\u4fa3\uff0c\u7406\u8bba\u4e0a\u90fd\u53ef\u4ee5\u68c0\u51fa\uff0c\u907f\u514d\u8fd9\u79cd\u5c0f\u6982\u7387\u610f\u5916\u3002<\/p>\n<\/div>
<\/span><\/div>\n2. PCR\u6280\u672f\u80fd\u4ee5\u8986\u76d6\u90e8\u5206\u7a81\u53d8\u7c7b\u578b<\/p>\n<\/div><\/h4><\/div>
PCR\u6280\u672f\u5bf9\u4e8e\u68c0\u6d4b\u70b9\u7a81\u53d8\u3001\u878d\u5408\u7a81\u53d8\u7684\u68c0\u6d4b\u80fd\u529b\u8f83\u5f3a\uff0c\u4f46\u76ee\u524d\u5404\u533b\u9662\u5e38\u7528\u7684PCR\u68c0\u6d4b\u8bd5\u5242\u76d2\u5bf9\u4e8e\u201c\u6269\u589e\u201d\u8fd9\u4e00\u7a81\u53d8\u7c7b\u578b\u57fa\u672c\u6ca1\u6709\u8986\u76d6\uff0c\u800c\u643a\u5e26HER2\u6269\u589e\u6216\u8005MET\u6269\u589e\u7684\u975e\u5c0f\u7ec6\u80de\u80ba\u764c\u60a3\u8005\u5206\u522b\u5360\u5230\u4e86\u6574\u4f53\u4eba\u7fa4\u76841-3%\uff0c\u82e5\u4f7f\u7528PCR\u6280\u672f\u53ef\u80fd\u5bfc\u81f4\u8fd9\u4e9b\u53ef\u7528\u836f\u7684\u9a71\u52a8\u57fa\u56e0\u7a81\u53d8\u7684\u60a3\u8005\u4f1a\u88ab\u9057\u6f0f\u3002<\/p>\n<\/div>
<\/span><\/div><\/div><\/div>\u5404\u4f4d\u53ef\u901a\u8fc7\u68c0\u67e5\u81ea\u5df1\u62ff\u5230\u7684\u533b\u9662\u7684\u68c0\u6d4b\u62a5\u544a\u4e0a\u68c0\u6d4b\u6280\u672f\u680f\u662f\u5426\u6709\u201c\u8367\u5149\u5b9a\u91cfPCR\u201d\u3001\u201cRT-PCR\u201d\u4ee5\u53ca\u201cARMS\u201d\u7b49\u5b57\u6837\uff0c\u82e5\u6709\u7684\u8bdd\u5219\u4e3aPCR\u6280\u672f\u3002\u5982\u679c\u5404\u4f4d\u4e00\u6b65\u4e00\u6b65\u6392\u67e5\u81ea\u8eab\u68c0\u6d4b\u6d41\u7a0b\uff0c\u786e\u8ba4\u81ea\u8eab\u764c\u79cd\u6709\u9776\u5411\u836f\u4e14\u4f7f\u7528\u4e86\u6b63\u786e\u5408\u683c\u7684\u7ec4\u7ec7\u6837\u672c\uff0c\u6700\u540e\u4f7f\u7528PCR\u6280\u672f\u68c0\u6d4b\u4e3a\u9634\u6027\uff0c\u90a3\u4e48\u6709\u6761\u4ef6\u7684\u5bb6\u5ead\u5728\u5269\u4f59\u6837\u672c\u5145\u8db3\u4ee5\u53ca\u7ecf\u6d4e\u5141\u8bb8\u7684\u60c5\u51b5\u4e0b\uff0c\u53ef\u4ee5\u8003\u8651\u518d\u5c1d\u8bd5NGS\u68c0\u6d4b\uff0c\u770b\u81ea\u8eab\u662f\u5426\u5c5e\u4e8e\u4e0a\u8ff0\u5c0f\u6982\u7387\u7f55\u89c1\u7a81\u53d8\u7c7b\u578b\u3002<\/p>\n<\/div>
\u603b\u7ed3<\/div><\/h4><\/div>\u6700\u540e\u5e2e\u5404\u4f4d\u68b3\u7406\u4e00\u4e0b\u82e5\u57fa\u56e0\u68c0\u6d4b\u62a5\u544a\u9634\u6027\uff0c\u4ece\u57fa\u56e0\u68c0\u6d4b\u89d2\u5ea6\uff0c\u8fd8\u53ef\u4ee5\u505a\u54ea\u4e9b\u5c1d\u8bd5\uff1a<\/p>\n
1. \u56de\u5f52\u75c5\u7406\u62a5\u544a\uff0c\u786e\u8ba4\u81ea\u8eab\u764c\u79cd\uff0c\u4e86\u89e3\u81ea\u8eab\u764c\u79cd\u662f\u5426\u6709\u5bf9\u5e94\u9776\u5411\u836f\u4ee5\u53ca\u9776\u5411\u57fa\u56e0\u7a81\u53d8\u53ef\u80fd\u6027\u5927\u5c0f\uff0c\u82e5\u65e0\u76f8\u5173\u9776\u5411\u836f\uff0c\u5219\u518d\u6b21\u57fa\u56e0\u68c0\u6d4b\u610f\u4e49\u4e0d\u5927\uff1b<\/p>\n
2. \u56de\u987e\u68c0\u6d4b\u4f7f\u7528\u7684\u6837\u672c\u7c7b\u578b\uff0c\u82e5\u4ec5\u4f7f\u7528\u8840\u6db2\u6837\u672c\u4e14\u672a\u68c0\u6d4b\u5230\u4efb\u4f55\u7a81\u53d8\uff0c\u5219\u6709\u6761\u4ef6\u7684\u60c5\u51b5\u4e0b\u53ef\u8003\u8651\u5c3d\u91cf\u83b7\u53d6\u7ec4\u7ec7\u6837\u672c\uff0c\u5728\u786e\u5b9a\u764c\u79cd\u7c7b\u578b\u9002\u5408\u540e\u7528\u7ec4\u7ec7\u6837\u672c\u518d\u884c\u68c0\u6d4b\uff1b<\/p>\n
3. \u56de\u987e\u68c0\u6d4b\u4f7f\u7528\u7684\u6837\u672c\u7c7b\u578b\uff0c\u82e5\u4f7f\u7528\u7684\u4e3a\u65b0\u9c9c\u6807\u672c\u6216\u6837\u672c\u91cf\u4e0d\u8db3\u7684\u77f3\u8721\u5207\u7247\uff0c\u5219\u4ecd\u662f\u56de\u5f52\u75c5\u7406\u62a5\u544a\uff0c\u5148\u786e\u8ba4\u6b64\u6b21\u53d6\u6837\u662f\u5426\u53d6\u5230\u80bf\u7624\u4ee5\u53ca\u80bf\u7624\u542b\u91cf\u662f\u5426\u8fbe\u6807\uff0c\u82e5\u4e0d\u7b26\u5408\u5219\u53ef\u8003\u8651\u91cd\u65b0\u53d6\u6837\uff0c\u8fbe\u6807\u540e\u518d\u9001\u68c0\uff0c\u82e5\u7b26\u5408\u6807\u51c6\u5219\u7ed3\u679c\u57fa\u672c\u53ef\u4fe1\uff0c\u518d\u884c\u68c0\u6d4b\u6539\u53d8\u53ef\u80fd\u6027\u4e0d\u5927\uff1b<\/p>\n
4. \u5ba1\u89c6\u68c0\u6d4b\u6280\u672f\uff0c\u82e5\u764c\u79cd\u3001\u6837\u672c\u5747\u65e0\u95ee\u9898\uff0c\u4f7f\u7528\u7684\u662fPCR\u6280\u672f\u68c0\u6d4b\uff0c\u7ecf\u6d4e\u5141\u8bb8\u4e14\u5269\u4f59\u6837\u672c\u8db3\u591f\u7684\u60c5\u51b5\u4e0b\u53ef\u8003\u8651\u518d\u884cNGS\u6280\u672f\u68c0\u6d4b\uff0c\u4ee5\u514d\u9057\u6f0f\u5c0f\u6982\u7387\u7684\u7f55\u89c1\u7a81\u53d8\u4f4d\u70b9\u53ca\u7c7b\u578b\u3002<\/p>\n
5. \u82e5\u764c\u79cd\u3001\u6837\u672c\uff0c\u8fd8\u6709\u68c0\u6d4b\u6280\u672f\u5747\u65e0\u95ee\u9898\uff0c\u90a3\u4e48\u57fa\u56e0\u68c0\u6d4b\u7ed3\u679c\u95ee\u9898\u4e0d\u5927\uff0c\u57fa\u672c\u4e0d\u4f1a\u6709\u9519\u6f0f\uff0c\u77ed\u671f\u5185\u4e0d\u9700\u8981\u4e8c\u6b21\u68c0\u6d4b\uff0c\u6539\u53d8\u53ef\u80fd\u6027\u4e5f\u4e0d\u9ad8\uff0c\u5b9e\u5728\u60f3\u640f\u4e00\u640f\u5efa\u8bae\u5728\u63a5\u53d7\u6b63\u89c4\u6cbb\u7597\u51fa\u73b0\u8fdb\u5c55\uff0c\u60c5\u51b5\u6709\u53d8\u5316\u4e4b\u540e\u624d\u8003\u8651\u3002<\/p>\n
\u672c\u6587\u7ae0\u8f6c\u8f7d\u4e8e\uff1a \u4e0e\u7231\u5171\u821e\u8ba2\u9605\u53f7<\/p>\n
\u539f\u6587\u94fe\u63a5\uff1ahttps:\/\/mp.weixin.qq.com\/s\/w6yaj-CImt27KZFIDDHHQw<\/p>\n<\/div><\/div><\/div><\/div><\/div><\/p>","protected":false},"excerpt":{"rendered":"
Patients who have just been diagnosed with non-small cell lung cancer have raised a common question: \"What should I do if the first genetic test shows no mutation? Do I need to retest?\" Regarding this issue, I would like to propose the following ideas from the perspective of genetic testing for your reference.<\/p>","protected":false},"author":1,"featured_media":3606,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"content-type":"","om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[202],"tags":[],"class_list":["post-1323","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-202"],"acf":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/posts\/1323","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/comments?post=1323"}],"version-history":[{"count":6,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/posts\/1323\/revisions"}],"predecessor-version":[{"id":3618,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/posts\/1323\/revisions\/3618"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/media\/3606"}],"wp:attachment":[{"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/media?parent=1323"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/categories?post=1323"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/tags?post=1323"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
1. \u7b2c\u4e00\u6b21\u68c0\u6d4b\u4f7f\u7528\u7684\u662f\u5426\u4e3a\u8840\u6db2\u6837\u672c<\/p>\n<\/div><\/h4><\/div>
\u672c\u4eba\u4ee5\u5f80\u7684\u6587\u7ae0\u4e2d\u66fe\u4ecb\u7ecd\u8fc7\u8840\u6db2\u6837\u672c\u7528\u4e8e\u57fa\u56e0\u68c0\u6d4b\u7684\u539f\u7406\uff08\u70b9\u51fb\u9605\u8bfb\u300aMRD\u2248\u8840\u6db2\u57fa\u56e0\u68c0\u6d4b\uff1f\u300b\uff09\uff0c\u4f9d\u9760\u7684\u662f\u8840\u6db2\u4e2d\u53ef\u80fd\u542b\u6709\u7684\u5faa\u73af\u80bf\u7624DNA\uff08ctDNA\uff09\uff0c\u4f46\u8840\u6db2\u6837\u672c\u6ce8\u5b9a\u65e0\u6cd5\u50cf\u7ec4\u7ec7\u6837\u672c\u90a3\u6837\u5236\u5907\u6210\u8721\u5757\u5728\u663e\u5fae\u955c\u4e0b\u505a\u75c5\u7406\u8bca\u65ad\uff0c\u786e\u8ba4\u764c\u79cd\u7c7b\u578b\uff0c\u4e5f\u65e0\u6cd5\u786e\u5b9a\u5176\u4e2dctDNA\u7684\u542b\u91cf\uff0c\u56e0\u6b64\u65e0\u8bba\u91c7\u7528\u73b0\u6709\u4f55\u79cd\u68c0\u6d4b\u6280\u672f\uff0c\u8840\u6db2\u6837\u672c\u90fd\u5b58\u5728\u542b\u91cf\u4e0d\u8db3\u5bfc\u81f4\u6f0f\u68c0\u7684\u53ef\u80fd\u6027\uff08\u8865\u5145\u4e00\u4e0b\uff0c\u6ca1\u6709\u54ea\u5bb6\u516c\u53f8\u80fd\u4fdd\u8bc1\u7528\u8840\u6db2\u7edd\u5bf9\u4e0d\u6f0f\u68c0\uff09\u3002<\/p>\n
\u6839\u636eNCCN\u975e\u5c0f\u7ec6\u80de\u80ba\u764c\u8bca\u7597\u6307\u5357\u4e2d\u7684\u7ecf\u9a8c\u7edf\u8ba1\uff0c\u8840\u6db2\u6837\u672c\u7684\u5047\u9634\u6027\uff08\u5373\u6f0f\u68c0\uff09\u6982\u7387\u7ea630%\u5de6\u53f3\uff0c\u537310\u540d\u5b9e\u9645\u6709\u7a81\u53d8\u7684\u60a3\u8005\u4e2d\u5c31\u7ea63\u540d\u60a3\u8005\u88ab\u6f0f\u6389[1]\u3002<\/p>\n
2020\u5e74\uff0c\u5929\u6d25\u533b\u79d1\u5927\u5b66\u53d1\u8868\u7814\u7a76\u56de\u987e2018\u5e74\u81f32021\u5e74\u671f\u95f4\u5929\u6d25\u533b\u79d1\u5927\u5b66\u603b\u533b\u9662\u80bf\u7624\u79d1\u6536\u6cbb\u7684NSCLC\u60a3\u8005\uff0c\u5176\u4e2d\u7eb3\u5165\u4e8632\u540d\u65e2\u6709\u7ec4\u7ec7\u6837\u672c\u4e5f\u6709\u8840\u6db2\u6837\u672c\u7684\u60a3\u8005\u5bf9\u6bd4\u4e24\u79cd\u6837\u672c\u7684\u68c0\u6d4b\u7ed3\u679c\uff0c\u572832\u4f8bNSCLC\u60a3\u8005\u4e2d\uff0c\u7ec4\u7ec7\u4e2d\u68c0\u6d4b\u51fa15\u4f8bEGFR\u7a81\u53d8\uff0c\u8840\u6d46\u4e2d\u68c0\u6d4b\u51fa13\u4f8b\u7a81\u53d8\uff08\u5373\u6709\u4e24\u540d\u5b9e\u9645\u6709EGFR\u7a81\u53d8\u7684\u60a3\u8005\u672a\u88ab\u8840\u6db2\u68c0\u6d4b\u51fa\uff09\uff0c\u7a81\u53d8\u4f4d\u70b9\u5b8c\u5168\u4e00\u81f4\u7684\u60a3\u8005\u517110\u4f8b\uff0c5\u4f8b\u60a3\u8005\u7ec4\u7ec7\u4e2d\u68c0\u6d4b\u51fa\u7684EGFR\u7a81\u53d8\u672a\u5728\u8840\u6d46\u4e2d\u68c0\u6d4b\u51fa\uff0c\u7ec4\u7ec7\u4e2d\u672a\u68c0\u6d4b\u5230\u7a81\u53d8\u7684\u8840\u6d46\u4e2d\u5747\u672a\u68c0\u6d4b\u5230\uff0c\u654f\u611f\u6027\u4e3a66.7%\uff0810\/15\uff09\uff0c\u7279\u5f02\u6027\u4e3a100%\uff0817\/17\uff09\uff0c\u4e24\u79cd\u6807\u672c\u68c0\u6d4b\u4e00\u81f4\u6027\u4e3a84.4%\uff0827\/32\uff09[2]\u3002\u7b80\u5355\u6765\u8bf4\uff0c\u8840\u6db2\u4e0d\u4ec5\u6709\u53ef\u80fd\u5c06\u5b9e\u9645\u6709\u7a81\u53d8\u7684\u60a3\u8005\u6f0f\u6389\uff0c\u8fd8\u6709\u53ef\u80fd\u68c0\u6d4b\u5230\u7684\u7a81\u53d8\u4f4d\u70b9\u5c11\u4e8e\u7ec4\u7ec7\u6837\u672c\u3002<\/p>\n
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<\/span><\/div>2. \u7b2c\u4e00\u6b21\u68c0\u6d4b\u4f7f\u7528\u7684\u7ec4\u7ec7\u6837\u672c\u662f\u5426\u7b26\u5408\u8d28\u63a7\u8981\u6c42<\/div><\/h4><\/div>\u672c\u4eba\u4ee5\u524d\u7684\u6587\u7ae0\u4e2d\u4e5f\u63d0\u53ca\u8fc7\u7ec4\u7ec7\u6837\u672c\u7684\u8d28\u91cf\u63a7\u5236\uff0c\u901a\u5e38\u7528\u4e8e\u57fa\u56e0\u68c0\u6d4b\u7684\u7ec4\u7ec7\u6837\u672c\u9700\u8981\u80bf\u7624\u6210\u5206\u542b\u91cf\u536020%\u4ee5\u4e0a\uff0c\u4e00\u822c\u68c0\u6d4b\u516c\u53f8\u9700\u898110-15\u5f20\u4e0d\u67d3\u8272\u7684\u5207\u7247\uff0c\u540c\u65f6\u5bf9\u4e8e\u4ece\u8fd9\u4e9b\u5207\u7247\u4e2d\u63d0\u53d6\u5230\u7684DNA\u603b\u91cf\u4e5f\u6709\u57fa\u672c\u8981\u6c42\uff0c\u5927\u81f4\u5982\u4e0b\u3002<\/p>\n<\/div>
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\uff08\u6ce8\u610f\uff1a\u901a\u5e38\u75c5\u7406\u62a5\u544a3-5\u4e2a\u5de5\u4f5c\u65e5\u5c31\u6709\u7ed3\u679c\uff0c\u9664\u4e86\u786e\u8bca\u764c\u79cd\u4ee5\u5916\uff0c\u8fd8\u53ef\u4ee5\u5927\u81f4\u4e86\u89e3\u6837\u672c\u4e2d\u80bf\u7624\u542b\u91cf\u591a\u5c11\uff0c\u4e0d\u4f1a\u803d\u8bef\u5404\u4f4d\u592a\u591a\u65f6\u95f4\u53cd\u800c\u8fd8\u80fd\u5e2e\u52a9\u4e86\u89e3\u6837\u672c\u8d28\u91cf\u597d\u574f\uff0c\u5c3d\u91cf\u4e0d\u8981\u7701\u7565\uff09<\/p>\n<\/div>
<\/span><\/div><\/div><\/div>\u5f71\u54cd\u56e0\u7d20\u4e09\uff1a\u68c0\u6d4b\u6280\u672f<\/div><\/h4><\/div>\u5f88\u591a\u5584\u4e8e\u5b66\u4e60\u7684\u75c5\u53cb\u5bf9\u4e8e\u68c0\u6d4b\u6280\u672f\u4e5f\u6709\u6240\u4e86\u89e3\uff0c\u5bf9\u4e8ePCR\u548cNGS\u6280\u672f\u5e76\u4e0d\u964c\u751f\uff0c\u6211\u4e4b\u524d\u4e5f\u5199\u8fc7\u300a\u9662\u5185\u9662\u5916\u57fa\u56e0\u68c0\u6d4b\u7684\u533a\u522b\u300b\uff08\u70b9\u51fb\u9605\u8bfb\uff09\uff0c\u5927\u90e8\u5206\u533b\u9662\u9662\u5185\u5747\u91c7\u7528PCR\u6280\u672f\uff0c\u65b9\u4fbf\u5feb\u6377\u4e14\u57fa\u672c\u80fd\u591f\u8986\u76d6\u9664NTRK\u4ee5\u5916\u7684\u5176\u4ed6\u9776\u5411\u57fa\u56e0\uff0c\u53ef\u4ee5\u6ee1\u8db3\u5927\u591a\u6570\u60a3\u8005\u7684\u68c0\u6d4b\u9700\u6c42\uff0c\u4e0d\u8fc7PCR\u6280\u672f\u5b58\u5728\u4e00\u5b9a\u7f3a\u9677\uff0c\u53ef\u80fd\u9020\u6210\u6781\u5c0f\u6982\u7387\u9057\u6f0f\u3002<\/p>\n<\/div>
\n1.PCR\u6280\u672f\u96be\u4ee5\u8986\u76d6\u9776\u5411\u57fa\u56e0\u7684\u7f55\u89c1\u7a81\u53d8\u578b<\/p>\n<\/div><\/h4><\/div>
\u901a\u4fd7\u4e00\u70b9\u8bf4\uff0cPCR\u6280\u672f\u5c31\u50cf\u662f\u62ff\u7740\u73b0\u6210\u7684\u6863\u6848\u53bb\u6392\u67e5\u5acc\u7591\u4eba\uff0c\u800cNGS\u6280\u672f\u5219\u662f\u5730\u6bef\u5f0f\u641c\u67e5\uff0c\u65e0\u8bba\u662f\u6863\u6848\u91cc\u7684\u8fd8\u662f\u6863\u6848\u5916\u7684\uff0c\u53ea\u8981\u68c0\u67e5\u53d1\u73b0\u6709\u95ee\u9898\u7684\u901a\u901a\u4e0a\u62a5\u3002PCR\u6280\u672f\u53ef\u7528\u4e8e\u68c0\u6d4b\u70b9\u7a81\u53d8\u4e0e\u878d\u5408\u7a81\u53d8\uff0c\u53ef\u8986\u76d6\u76ee\u6807\u57fa\u56e0\u7684\u5404\u79cd\u5df2\u77e5\u7a81\u53d8\u7c7b\u578b\uff0c\u4f46\u5bf9\u4e8e\u672a\u77e5\u53ca\u7f55\u89c1\u7a81\u53d8\u4f4d\u70b9\u5219\u7565\u663e\u65e0\u529b\u3002<\/p>\n
\u4ee5\u6700\u5e38\u89c1\u7684EGFR\u57fa\u56e0\u68c0\u6d4b\u4e3a\u4f8b\uff0c\u76ee\u524d\u56fd\u5185\u5df2\u83b7\u6279\u7684PCR\u8bd5\u5242\u76d2\u7684\u68c0\u6d4b\u4f4d\u70b9\u4e3b\u8981\u5305\u62ec\u5404\u7c7bEGFR 19\u7f3a\u5931\u7a81\u53d8\u3001EGFR L858R\u7a81\u53d8\u3001EGFR T790M\u7a81\u53d8\uff0cEGFR 20\u63d2\u5165\u4ee5\u53ca\u90e8\u5206\u4f4d\u70b9\u3002<\/p>\n
\u867d\u7136\u8fd9\u4e9b\u5df2\u7ecf\u8986\u76d6\u4e86\u8d85\u8fc790%\u7684EGFR\u7a81\u53d8\uff0c\u4f46EGFR\u57fa\u56e0\u768418\u300119\u300120\u300121\u5916\u663e\u5b50\u5185\u4ecd\u53ef\u80fd\u51fa\u73b0\u5982EGFR C797S\u3001EGFR L833V\u3001EGFR L747Q\u7b49\u7b49\u66f4\u52a0\u5c11\u89c1\u7684\u7a81\u53d8\u4f4d\u70b9\u3002\u8fd9\u4e9b\u7a81\u53d8\u6216\u51fa\u73b0\u7684\u6982\u7387\u6781\u4f4e\uff08\u5373\u7f55\u89c1\u7a81\u53d8\u4f4d\u70b9\uff09\u6216\u7279\u6b8a\u60c5\u51b5\u4e0b\u53ef\u80fd\u51fa\u73b0\uff0c\u53ef\u80fd\u4ec5\u5360\u6240\u6709EGFR\u7a81\u53d8\u76841-5%\u5de6\u53f3\uff08\u8f83\u96be\u6709\u5b8c\u5168\u7edf\u8ba1\uff09\uff0c\u5bf9\u4e8e\u8fd9\u4e9b\u4e0d\u5728\u68c0\u6d4b\u8303\u56f4\u5185\u7684\u7a81\u53d8\u4f4d\u70b9\uff0cPCR\u6280\u672f\u65e0\u80fd\u4e3a\u529b\uff0c\u4f46NGS\u6280\u672f\u5219\u6709\u53ef\u80fd\u68c0\u51fa\u3002<\/p>\n<\/div>
<\/span><\/div>\u518d\u4ee5\u88ab\u79f0\u4e3a\u201c\u94bb\u77f3\u7a81\u53d8\u201d\u7684ALK\u878d\u5408\u7a81\u53d8\u4e3a\u4f8b\uff0c\u8be5\u7a81\u53d8\u7c7b\u578b\u4e0d\u540c\u4e8e\u4e0a\u8ff0\u7684EGFR\u70b9\u7a81\u53d8\uff0c\u5e76\u4e0d\u662f\u5355\u4e2a\u57fa\u56e0\u7684\u4e8b\uff0c\u5b83\u662f\u4e24\u4e2a\u57fa\u56e0\u95f4\u7684\u65ad\u88c2\u4e0e\u878d\u5408\uff0c\u6211\u4eec\u5c06\u8fd9\u79cd\u878d\u5408\u7684\u4e24\u4e2a\u57fa\u56e0\u6210\u4e3a\u201c\u878d\u5408\u4f34\u4fa3\u201d\uff0cALK\u878d\u5408\u5373\u662f\u6307\u5176\u4e2d\u4e00\u4e2a\u4f34\u4fa3\u56fa\u5b9a\u4e3aALK\u57fa\u56e0\uff0c\u53e6\u4e00\u534a\u53ef\u80fd\u4f1a\u6709\u591a\u79cd\u3002<\/p>\n
\u6700\u5e38\u89c1\u7684ALK\u878d\u5408\u53d1\u751f\u5728EML4\u4e0eALK\u57fa\u56e0\u4e4b\u95f4\uff0c\u4f46\u968f\u7740\u7814\u7a76\u53d1\u5c55\uff0c\u6211\u4eec\u53d1\u73b0\u4e86\u66f4\u591a\u4f34\u4fa3\u57fa\u56e0\uff0c\u8f83\u65e9\u4ee5\u524d\u7684PCR\u8bd5\u5242\u76d2\u4ec5\u53ef\u68c0\u6d4bEML4-ALK\u878d\u5408\u7c7b\u578b\uff0c\u5176\u4ed6\u878d\u5408\u4f34\u4fa3\u5f62\u6210\u7684ALK\u878d\u5408\u5219\u65e0\u6cd5\u53d1\u73b0\uff0c\u73b0\u5728\u6709\u4e9b\u66f4\u65b0\u7684PCR\u8bd5\u5242\u76d2\u9664EML4\u57fa\u56e0\u4ee5\u5916\uff0c\u8fd8\u5305\u542bKIF5B\u3001KCL1\u3001TFG\u7b49\u6982\u7387\u76f8\u5bf9\u8f83\u4f4e\u7684\u878d\u5408\u4f34\u4fa3\uff0c\u8fdb\u4e00\u6b65\u6269\u5927\u4e86PCR\u6280\u672f\u53ef\u68c0\u6d4b\u5230\u7684ALK\u878d\u5408\u7c7b\u578b\u3002<\/p>\n
\u53ef\u4ee5\u8bf490%\u751a\u81f395%\u4ee5\u4e0a\u7684ALK\u878d\u5408\u60a3\u8005\u90fd\u53ef\u901a\u8fc7PCR\u6280\u672f\u53d1\u73b0\uff0c\u4f46ALK\u57fa\u56e0\u7684\u878d\u5408\u4f34\u4fa3\u4ecd\u7136\u8d85\u51fa\u6b64\u8303\u56f4\uff0c\u4f8b\u5982\u6211\u66fe\u9047\u5230\u8fc7\u67d0\u4e2a\u75c5\u53cb\u662fDIRC3-ALK\u878d\u5408\uff0cNGS\u6280\u672f\u7684\u7279\u6027\u8ba9\u5b83\u4e0d\u53d7\u8fd9\u79cd\u8303\u56f4\u9650\u5236\uff0c\u65e0\u8bba\u54ea\u79cd\u878d\u5408\u4f34\u4fa3\uff0c\u7406\u8bba\u4e0a\u90fd\u53ef\u4ee5\u68c0\u51fa\uff0c\u907f\u514d\u8fd9\u79cd\u5c0f\u6982\u7387\u610f\u5916\u3002<\/p>\n<\/div>
<\/span><\/div>\n2. PCR\u6280\u672f\u80fd\u4ee5\u8986\u76d6\u90e8\u5206\u7a81\u53d8\u7c7b\u578b<\/p>\n<\/div><\/h4><\/div>
PCR\u6280\u672f\u5bf9\u4e8e\u68c0\u6d4b\u70b9\u7a81\u53d8\u3001\u878d\u5408\u7a81\u53d8\u7684\u68c0\u6d4b\u80fd\u529b\u8f83\u5f3a\uff0c\u4f46\u76ee\u524d\u5404\u533b\u9662\u5e38\u7528\u7684PCR\u68c0\u6d4b\u8bd5\u5242\u76d2\u5bf9\u4e8e\u201c\u6269\u589e\u201d\u8fd9\u4e00\u7a81\u53d8\u7c7b\u578b\u57fa\u672c\u6ca1\u6709\u8986\u76d6\uff0c\u800c\u643a\u5e26HER2\u6269\u589e\u6216\u8005MET\u6269\u589e\u7684\u975e\u5c0f\u7ec6\u80de\u80ba\u764c\u60a3\u8005\u5206\u522b\u5360\u5230\u4e86\u6574\u4f53\u4eba\u7fa4\u76841-3%\uff0c\u82e5\u4f7f\u7528PCR\u6280\u672f\u53ef\u80fd\u5bfc\u81f4\u8fd9\u4e9b\u53ef\u7528\u836f\u7684\u9a71\u52a8\u57fa\u56e0\u7a81\u53d8\u7684\u60a3\u8005\u4f1a\u88ab\u9057\u6f0f\u3002<\/p>\n<\/div>
<\/span><\/div><\/div><\/div>\u5404\u4f4d\u53ef\u901a\u8fc7\u68c0\u67e5\u81ea\u5df1\u62ff\u5230\u7684\u533b\u9662\u7684\u68c0\u6d4b\u62a5\u544a\u4e0a\u68c0\u6d4b\u6280\u672f\u680f\u662f\u5426\u6709\u201c\u8367\u5149\u5b9a\u91cfPCR\u201d\u3001\u201cRT-PCR\u201d\u4ee5\u53ca\u201cARMS\u201d\u7b49\u5b57\u6837\uff0c\u82e5\u6709\u7684\u8bdd\u5219\u4e3aPCR\u6280\u672f\u3002\u5982\u679c\u5404\u4f4d\u4e00\u6b65\u4e00\u6b65\u6392\u67e5\u81ea\u8eab\u68c0\u6d4b\u6d41\u7a0b\uff0c\u786e\u8ba4\u81ea\u8eab\u764c\u79cd\u6709\u9776\u5411\u836f\u4e14\u4f7f\u7528\u4e86\u6b63\u786e\u5408\u683c\u7684\u7ec4\u7ec7\u6837\u672c\uff0c\u6700\u540e\u4f7f\u7528PCR\u6280\u672f\u68c0\u6d4b\u4e3a\u9634\u6027\uff0c\u90a3\u4e48\u6709\u6761\u4ef6\u7684\u5bb6\u5ead\u5728\u5269\u4f59\u6837\u672c\u5145\u8db3\u4ee5\u53ca\u7ecf\u6d4e\u5141\u8bb8\u7684\u60c5\u51b5\u4e0b\uff0c\u53ef\u4ee5\u8003\u8651\u518d\u5c1d\u8bd5NGS\u68c0\u6d4b\uff0c\u770b\u81ea\u8eab\u662f\u5426\u5c5e\u4e8e\u4e0a\u8ff0\u5c0f\u6982\u7387\u7f55\u89c1\u7a81\u53d8\u7c7b\u578b\u3002<\/p>\n<\/div>
\u603b\u7ed3<\/div><\/h4><\/div>\u6700\u540e\u5e2e\u5404\u4f4d\u68b3\u7406\u4e00\u4e0b\u82e5\u57fa\u56e0\u68c0\u6d4b\u62a5\u544a\u9634\u6027\uff0c\u4ece\u57fa\u56e0\u68c0\u6d4b\u89d2\u5ea6\uff0c\u8fd8\u53ef\u4ee5\u505a\u54ea\u4e9b\u5c1d\u8bd5\uff1a<\/p>\n
1. \u56de\u5f52\u75c5\u7406\u62a5\u544a\uff0c\u786e\u8ba4\u81ea\u8eab\u764c\u79cd\uff0c\u4e86\u89e3\u81ea\u8eab\u764c\u79cd\u662f\u5426\u6709\u5bf9\u5e94\u9776\u5411\u836f\u4ee5\u53ca\u9776\u5411\u57fa\u56e0\u7a81\u53d8\u53ef\u80fd\u6027\u5927\u5c0f\uff0c\u82e5\u65e0\u76f8\u5173\u9776\u5411\u836f\uff0c\u5219\u518d\u6b21\u57fa\u56e0\u68c0\u6d4b\u610f\u4e49\u4e0d\u5927\uff1b<\/p>\n
2. \u56de\u987e\u68c0\u6d4b\u4f7f\u7528\u7684\u6837\u672c\u7c7b\u578b\uff0c\u82e5\u4ec5\u4f7f\u7528\u8840\u6db2\u6837\u672c\u4e14\u672a\u68c0\u6d4b\u5230\u4efb\u4f55\u7a81\u53d8\uff0c\u5219\u6709\u6761\u4ef6\u7684\u60c5\u51b5\u4e0b\u53ef\u8003\u8651\u5c3d\u91cf\u83b7\u53d6\u7ec4\u7ec7\u6837\u672c\uff0c\u5728\u786e\u5b9a\u764c\u79cd\u7c7b\u578b\u9002\u5408\u540e\u7528\u7ec4\u7ec7\u6837\u672c\u518d\u884c\u68c0\u6d4b\uff1b<\/p>\n
3. \u56de\u987e\u68c0\u6d4b\u4f7f\u7528\u7684\u6837\u672c\u7c7b\u578b\uff0c\u82e5\u4f7f\u7528\u7684\u4e3a\u65b0\u9c9c\u6807\u672c\u6216\u6837\u672c\u91cf\u4e0d\u8db3\u7684\u77f3\u8721\u5207\u7247\uff0c\u5219\u4ecd\u662f\u56de\u5f52\u75c5\u7406\u62a5\u544a\uff0c\u5148\u786e\u8ba4\u6b64\u6b21\u53d6\u6837\u662f\u5426\u53d6\u5230\u80bf\u7624\u4ee5\u53ca\u80bf\u7624\u542b\u91cf\u662f\u5426\u8fbe\u6807\uff0c\u82e5\u4e0d\u7b26\u5408\u5219\u53ef\u8003\u8651\u91cd\u65b0\u53d6\u6837\uff0c\u8fbe\u6807\u540e\u518d\u9001\u68c0\uff0c\u82e5\u7b26\u5408\u6807\u51c6\u5219\u7ed3\u679c\u57fa\u672c\u53ef\u4fe1\uff0c\u518d\u884c\u68c0\u6d4b\u6539\u53d8\u53ef\u80fd\u6027\u4e0d\u5927\uff1b<\/p>\n
4. \u5ba1\u89c6\u68c0\u6d4b\u6280\u672f\uff0c\u82e5\u764c\u79cd\u3001\u6837\u672c\u5747\u65e0\u95ee\u9898\uff0c\u4f7f\u7528\u7684\u662fPCR\u6280\u672f\u68c0\u6d4b\uff0c\u7ecf\u6d4e\u5141\u8bb8\u4e14\u5269\u4f59\u6837\u672c\u8db3\u591f\u7684\u60c5\u51b5\u4e0b\u53ef\u8003\u8651\u518d\u884cNGS\u6280\u672f\u68c0\u6d4b\uff0c\u4ee5\u514d\u9057\u6f0f\u5c0f\u6982\u7387\u7684\u7f55\u89c1\u7a81\u53d8\u4f4d\u70b9\u53ca\u7c7b\u578b\u3002<\/p>\n
5. \u82e5\u764c\u79cd\u3001\u6837\u672c\uff0c\u8fd8\u6709\u68c0\u6d4b\u6280\u672f\u5747\u65e0\u95ee\u9898\uff0c\u90a3\u4e48\u57fa\u56e0\u68c0\u6d4b\u7ed3\u679c\u95ee\u9898\u4e0d\u5927\uff0c\u57fa\u672c\u4e0d\u4f1a\u6709\u9519\u6f0f\uff0c\u77ed\u671f\u5185\u4e0d\u9700\u8981\u4e8c\u6b21\u68c0\u6d4b\uff0c\u6539\u53d8\u53ef\u80fd\u6027\u4e5f\u4e0d\u9ad8\uff0c\u5b9e\u5728\u60f3\u640f\u4e00\u640f\u5efa\u8bae\u5728\u63a5\u53d7\u6b63\u89c4\u6cbb\u7597\u51fa\u73b0\u8fdb\u5c55\uff0c\u60c5\u51b5\u6709\u53d8\u5316\u4e4b\u540e\u624d\u8003\u8651\u3002<\/p>\n
\u672c\u6587\u7ae0\u8f6c\u8f7d\u4e8e\uff1a \u4e0e\u7231\u5171\u821e\u8ba2\u9605\u53f7<\/p>\n
\u539f\u6587\u94fe\u63a5\uff1ahttps:\/\/mp.weixin.qq.com\/s\/w6yaj-CImt27KZFIDDHHQw<\/p>\n<\/div><\/div><\/div><\/div><\/div><\/p>","protected":false},"excerpt":{"rendered":"
Patients who have just been diagnosed with non-small cell lung cancer have raised a common question: \"What should I do if the first genetic test shows no mutation? Do I need to retest?\" Regarding this issue, I would like to propose the following ideas from the perspective of genetic testing for your reference.<\/p>","protected":false},"author":1,"featured_media":3606,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"content-type":"","om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[202],"tags":[],"class_list":["post-1323","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-202"],"acf":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/posts\/1323","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/comments?post=1323"}],"version-history":[{"count":6,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/posts\/1323\/revisions"}],"predecessor-version":[{"id":3618,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/posts\/1323\/revisions\/3618"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/media\/3606"}],"wp:attachment":[{"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/media?parent=1323"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/categories?post=1323"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/tags?post=1323"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
\u672c\u4eba\u4ee5\u524d\u7684\u6587\u7ae0\u4e2d\u4e5f\u63d0\u53ca\u8fc7\u7ec4\u7ec7\u6837\u672c\u7684\u8d28\u91cf\u63a7\u5236\uff0c\u901a\u5e38\u7528\u4e8e\u57fa\u56e0\u68c0\u6d4b\u7684\u7ec4\u7ec7\u6837\u672c\u9700\u8981\u80bf\u7624\u6210\u5206\u542b\u91cf\u536020%\u4ee5\u4e0a\uff0c\u4e00\u822c\u68c0\u6d4b\u516c\u53f8\u9700\u898110-15\u5f20\u4e0d\u67d3\u8272\u7684\u5207\u7247\uff0c\u540c\u65f6\u5bf9\u4e8e\u4ece\u8fd9\u4e9b\u5207\u7247\u4e2d\u63d0\u53d6\u5230\u7684DNA\u603b\u91cf\u4e5f\u6709\u57fa\u672c\u8981\u6c42\uff0c\u5927\u81f4\u5982\u4e0b\u3002<\/p>\n<\/div>
<\/span><\/div>\u5927\u591a\u6570\u60c5\u51b5\u4e0b\u4f7f\u7528\u7ec4\u7ec7\u6837\u672c\u68c0\u6d4b\u5f88\u5c11\u4f1a\u6709\u9519\u6f0f\uff0c\u4f46\u4ecd\u5b58\u5728\u5c0f\u6982\u7387\u610f\u5916\u60c5\u51b5\uff0c\u901a\u5e38\u662f\u7531\u4e8e\u7126\u6025\u5fc3\u7406\uff0c\u8d2a\u56fe\u201c\u5feb\u201d\u5b57\u60f3\u5c3d\u5feb\u62ff\u5230\u7ed3\u679c\u5f00\u59cb\u6cbb\u7597\uff0c\u505a\u7a7f\u523a\u6216\u8005\u6c14\u7ba1\u955c\u7684\u540c\u65f6\u53d6\u4e24\u4efd\uff0c\u4e00\u4efd\u9001\u75c5\u7406\u79d1\u505a\u75c5\u7406\u8bca\u65ad\uff0c\u4e00\u4efd\u9001\u68c0\u6d4b\u516c\u53f8\uff0c\u6216\u8005\u5e72\u8106\u53ea\u53d6\u4e00\u4efd\u7ec4\u7ec7\u76f4\u63a5\u9001\u68c0\u6d4b\u516c\u53f8\u3002\u8fd9\u6837\u5c31\u7ed9\u68c0\u6d4b\u5e26\u6765\u4e86\u4e0d\u786e\u5b9a\u6027\u3002<\/p>\n
\u9996\u5148\u8fd8\u662f\u764c\u79cd\u7684\u95ee\u9898\uff0c\u5404\u4f4d\u4e00\u5b9a\u8981\u8bb0\u4f4f\u5e76\u975e\u6240\u6709\u764c\u79cd\u90fd\u6709\u5bf9\u5e94\u9776\u5411\u836f\uff0c\u5e76\u975e\u90fd\u5fc5\u987b\u57fa\u56e0\u68c0\u6d4b\uff1b\u5176\u6b21\u662f\u7a7f\u523a\u6216\u8005\u6c14\u7ba1\u955c\u5e76\u975e\u6bcf\u6b21\u90fd\u80fd\u53d6\u5230\u60f3\u8981\u7684\u80bf\u7624\u7ec4\u7ec7\uff0c\u6709\u53ef\u80fd\u53ea\u662f\u708e\u75c7\u7ec6\u80de\u6216\u8005\u6781\u5c11\u91cf\u80bf\u7624\u7ec6\u80de\uff0c\u56e0\u6b64\u8fd9\u79cd\u201c\u4e0d\u7b49\u75c5\u7406\u62a5\u544a\u5c31\u76f4\u63a5\u9001\u65b0\u9c9c\u7ec4\u7ec7\u505a\u57fa\u56e0\u68c0\u6d4b\u201d\u7684\u60c5\u51b5\uff0c\u82e5\u68c0\u6d4b\u9633\u6027\uff0c\u6709\u5bf9\u5e94\u9776\u5411\u836f\u90a3\u6700\u597d\uff0c\u82e5\u68c0\u6d4b\u9634\u6027\uff0c\u5219\u4e0d\u80fd\u786e\u5b9a\u662f\u56e0\u4e3a\u81ea\u8eab\u764c\u79cd\u6ca1\u6709\u9776\u5411\u836f\u5bfc\u81f4\u68c0\u6d4b\u4e0d\u5230\u9776\u5411\u57fa\u56e0\u7a81\u53d8\uff0c\u8fd8\u662f\u56e0\u4e3a\u53d6\u6837\u91cf\u4e0d\u591f\u6837\u672c\u8d28\u91cf\u4e0d\u8fc7\u5173\u5bfc\u81f4\u6f0f\u68c0\u3002<\/p>\n
\u7ec4\u7ec7\u6837\u672c\u5e76\u975e\u4e07\u65e0\u4e00\u5931\uff0c\u82e5\u4f7f\u7528\u7ec4\u7ec7\u6837\u672c\u68c0\u6d4b\u4e5f\u4e3a\u9634\u6027\uff0c\u9700\u5ba1\u89c6\u4e00\u4e0b\u81ea\u8eab\u662f\u5426\u72af\u4e86\u4e0a\u8ff0\u7126\u6025\u5f15\u8d77\u7684\u9519\u8bef\uff0c\u4e07\u4e00\u7b26\u5408\u5219\u53ef\u8003\u8651\u91cd\u65b0\u53d6\u6837\u6216\u8005\u7b49\u75c5\u7406\u8bca\u65ad\u51fa\u6765\u4e4b\u540e\u518d\u6b21\u68c0\u6d4b\u3002<\/p>\n
\uff08\u6ce8\u610f\uff1a\u901a\u5e38\u75c5\u7406\u62a5\u544a3-5\u4e2a\u5de5\u4f5c\u65e5\u5c31\u6709\u7ed3\u679c\uff0c\u9664\u4e86\u786e\u8bca\u764c\u79cd\u4ee5\u5916\uff0c\u8fd8\u53ef\u4ee5\u5927\u81f4\u4e86\u89e3\u6837\u672c\u4e2d\u80bf\u7624\u542b\u91cf\u591a\u5c11\uff0c\u4e0d\u4f1a\u803d\u8bef\u5404\u4f4d\u592a\u591a\u65f6\u95f4\u53cd\u800c\u8fd8\u80fd\u5e2e\u52a9\u4e86\u89e3\u6837\u672c\u8d28\u91cf\u597d\u574f\uff0c\u5c3d\u91cf\u4e0d\u8981\u7701\u7565\uff09<\/p>\n<\/div>
<\/span><\/div><\/div><\/div>\u5f71\u54cd\u56e0\u7d20\u4e09\uff1a\u68c0\u6d4b\u6280\u672f<\/div><\/h4><\/div>\u5f88\u591a\u5584\u4e8e\u5b66\u4e60\u7684\u75c5\u53cb\u5bf9\u4e8e\u68c0\u6d4b\u6280\u672f\u4e5f\u6709\u6240\u4e86\u89e3\uff0c\u5bf9\u4e8ePCR\u548cNGS\u6280\u672f\u5e76\u4e0d\u964c\u751f\uff0c\u6211\u4e4b\u524d\u4e5f\u5199\u8fc7\u300a\u9662\u5185\u9662\u5916\u57fa\u56e0\u68c0\u6d4b\u7684\u533a\u522b\u300b\uff08\u70b9\u51fb\u9605\u8bfb\uff09\uff0c\u5927\u90e8\u5206\u533b\u9662\u9662\u5185\u5747\u91c7\u7528PCR\u6280\u672f\uff0c\u65b9\u4fbf\u5feb\u6377\u4e14\u57fa\u672c\u80fd\u591f\u8986\u76d6\u9664NTRK\u4ee5\u5916\u7684\u5176\u4ed6\u9776\u5411\u57fa\u56e0\uff0c\u53ef\u4ee5\u6ee1\u8db3\u5927\u591a\u6570\u60a3\u8005\u7684\u68c0\u6d4b\u9700\u6c42\uff0c\u4e0d\u8fc7PCR\u6280\u672f\u5b58\u5728\u4e00\u5b9a\u7f3a\u9677\uff0c\u53ef\u80fd\u9020\u6210\u6781\u5c0f\u6982\u7387\u9057\u6f0f\u3002<\/p>\n<\/div>
\n1.PCR\u6280\u672f\u96be\u4ee5\u8986\u76d6\u9776\u5411\u57fa\u56e0\u7684\u7f55\u89c1\u7a81\u53d8\u578b<\/p>\n<\/div><\/h4><\/div>
\u901a\u4fd7\u4e00\u70b9\u8bf4\uff0cPCR\u6280\u672f\u5c31\u50cf\u662f\u62ff\u7740\u73b0\u6210\u7684\u6863\u6848\u53bb\u6392\u67e5\u5acc\u7591\u4eba\uff0c\u800cNGS\u6280\u672f\u5219\u662f\u5730\u6bef\u5f0f\u641c\u67e5\uff0c\u65e0\u8bba\u662f\u6863\u6848\u91cc\u7684\u8fd8\u662f\u6863\u6848\u5916\u7684\uff0c\u53ea\u8981\u68c0\u67e5\u53d1\u73b0\u6709\u95ee\u9898\u7684\u901a\u901a\u4e0a\u62a5\u3002PCR\u6280\u672f\u53ef\u7528\u4e8e\u68c0\u6d4b\u70b9\u7a81\u53d8\u4e0e\u878d\u5408\u7a81\u53d8\uff0c\u53ef\u8986\u76d6\u76ee\u6807\u57fa\u56e0\u7684\u5404\u79cd\u5df2\u77e5\u7a81\u53d8\u7c7b\u578b\uff0c\u4f46\u5bf9\u4e8e\u672a\u77e5\u53ca\u7f55\u89c1\u7a81\u53d8\u4f4d\u70b9\u5219\u7565\u663e\u65e0\u529b\u3002<\/p>\n
\u4ee5\u6700\u5e38\u89c1\u7684EGFR\u57fa\u56e0\u68c0\u6d4b\u4e3a\u4f8b\uff0c\u76ee\u524d\u56fd\u5185\u5df2\u83b7\u6279\u7684PCR\u8bd5\u5242\u76d2\u7684\u68c0\u6d4b\u4f4d\u70b9\u4e3b\u8981\u5305\u62ec\u5404\u7c7bEGFR 19\u7f3a\u5931\u7a81\u53d8\u3001EGFR L858R\u7a81\u53d8\u3001EGFR T790M\u7a81\u53d8\uff0cEGFR 20\u63d2\u5165\u4ee5\u53ca\u90e8\u5206\u4f4d\u70b9\u3002<\/p>\n
\u867d\u7136\u8fd9\u4e9b\u5df2\u7ecf\u8986\u76d6\u4e86\u8d85\u8fc790%\u7684EGFR\u7a81\u53d8\uff0c\u4f46EGFR\u57fa\u56e0\u768418\u300119\u300120\u300121\u5916\u663e\u5b50\u5185\u4ecd\u53ef\u80fd\u51fa\u73b0\u5982EGFR C797S\u3001EGFR L833V\u3001EGFR L747Q\u7b49\u7b49\u66f4\u52a0\u5c11\u89c1\u7684\u7a81\u53d8\u4f4d\u70b9\u3002\u8fd9\u4e9b\u7a81\u53d8\u6216\u51fa\u73b0\u7684\u6982\u7387\u6781\u4f4e\uff08\u5373\u7f55\u89c1\u7a81\u53d8\u4f4d\u70b9\uff09\u6216\u7279\u6b8a\u60c5\u51b5\u4e0b\u53ef\u80fd\u51fa\u73b0\uff0c\u53ef\u80fd\u4ec5\u5360\u6240\u6709EGFR\u7a81\u53d8\u76841-5%\u5de6\u53f3\uff08\u8f83\u96be\u6709\u5b8c\u5168\u7edf\u8ba1\uff09\uff0c\u5bf9\u4e8e\u8fd9\u4e9b\u4e0d\u5728\u68c0\u6d4b\u8303\u56f4\u5185\u7684\u7a81\u53d8\u4f4d\u70b9\uff0cPCR\u6280\u672f\u65e0\u80fd\u4e3a\u529b\uff0c\u4f46NGS\u6280\u672f\u5219\u6709\u53ef\u80fd\u68c0\u51fa\u3002<\/p>\n<\/div>
<\/span><\/div>\u518d\u4ee5\u88ab\u79f0\u4e3a\u201c\u94bb\u77f3\u7a81\u53d8\u201d\u7684ALK\u878d\u5408\u7a81\u53d8\u4e3a\u4f8b\uff0c\u8be5\u7a81\u53d8\u7c7b\u578b\u4e0d\u540c\u4e8e\u4e0a\u8ff0\u7684EGFR\u70b9\u7a81\u53d8\uff0c\u5e76\u4e0d\u662f\u5355\u4e2a\u57fa\u56e0\u7684\u4e8b\uff0c\u5b83\u662f\u4e24\u4e2a\u57fa\u56e0\u95f4\u7684\u65ad\u88c2\u4e0e\u878d\u5408\uff0c\u6211\u4eec\u5c06\u8fd9\u79cd\u878d\u5408\u7684\u4e24\u4e2a\u57fa\u56e0\u6210\u4e3a\u201c\u878d\u5408\u4f34\u4fa3\u201d\uff0cALK\u878d\u5408\u5373\u662f\u6307\u5176\u4e2d\u4e00\u4e2a\u4f34\u4fa3\u56fa\u5b9a\u4e3aALK\u57fa\u56e0\uff0c\u53e6\u4e00\u534a\u53ef\u80fd\u4f1a\u6709\u591a\u79cd\u3002<\/p>\n
\u6700\u5e38\u89c1\u7684ALK\u878d\u5408\u53d1\u751f\u5728EML4\u4e0eALK\u57fa\u56e0\u4e4b\u95f4\uff0c\u4f46\u968f\u7740\u7814\u7a76\u53d1\u5c55\uff0c\u6211\u4eec\u53d1\u73b0\u4e86\u66f4\u591a\u4f34\u4fa3\u57fa\u56e0\uff0c\u8f83\u65e9\u4ee5\u524d\u7684PCR\u8bd5\u5242\u76d2\u4ec5\u53ef\u68c0\u6d4bEML4-ALK\u878d\u5408\u7c7b\u578b\uff0c\u5176\u4ed6\u878d\u5408\u4f34\u4fa3\u5f62\u6210\u7684ALK\u878d\u5408\u5219\u65e0\u6cd5\u53d1\u73b0\uff0c\u73b0\u5728\u6709\u4e9b\u66f4\u65b0\u7684PCR\u8bd5\u5242\u76d2\u9664EML4\u57fa\u56e0\u4ee5\u5916\uff0c\u8fd8\u5305\u542bKIF5B\u3001KCL1\u3001TFG\u7b49\u6982\u7387\u76f8\u5bf9\u8f83\u4f4e\u7684\u878d\u5408\u4f34\u4fa3\uff0c\u8fdb\u4e00\u6b65\u6269\u5927\u4e86PCR\u6280\u672f\u53ef\u68c0\u6d4b\u5230\u7684ALK\u878d\u5408\u7c7b\u578b\u3002<\/p>\n
\u53ef\u4ee5\u8bf490%\u751a\u81f395%\u4ee5\u4e0a\u7684ALK\u878d\u5408\u60a3\u8005\u90fd\u53ef\u901a\u8fc7PCR\u6280\u672f\u53d1\u73b0\uff0c\u4f46ALK\u57fa\u56e0\u7684\u878d\u5408\u4f34\u4fa3\u4ecd\u7136\u8d85\u51fa\u6b64\u8303\u56f4\uff0c\u4f8b\u5982\u6211\u66fe\u9047\u5230\u8fc7\u67d0\u4e2a\u75c5\u53cb\u662fDIRC3-ALK\u878d\u5408\uff0cNGS\u6280\u672f\u7684\u7279\u6027\u8ba9\u5b83\u4e0d\u53d7\u8fd9\u79cd\u8303\u56f4\u9650\u5236\uff0c\u65e0\u8bba\u54ea\u79cd\u878d\u5408\u4f34\u4fa3\uff0c\u7406\u8bba\u4e0a\u90fd\u53ef\u4ee5\u68c0\u51fa\uff0c\u907f\u514d\u8fd9\u79cd\u5c0f\u6982\u7387\u610f\u5916\u3002<\/p>\n<\/div>
<\/span><\/div>\n2. PCR\u6280\u672f\u80fd\u4ee5\u8986\u76d6\u90e8\u5206\u7a81\u53d8\u7c7b\u578b<\/p>\n<\/div><\/h4><\/div>
PCR\u6280\u672f\u5bf9\u4e8e\u68c0\u6d4b\u70b9\u7a81\u53d8\u3001\u878d\u5408\u7a81\u53d8\u7684\u68c0\u6d4b\u80fd\u529b\u8f83\u5f3a\uff0c\u4f46\u76ee\u524d\u5404\u533b\u9662\u5e38\u7528\u7684PCR\u68c0\u6d4b\u8bd5\u5242\u76d2\u5bf9\u4e8e\u201c\u6269\u589e\u201d\u8fd9\u4e00\u7a81\u53d8\u7c7b\u578b\u57fa\u672c\u6ca1\u6709\u8986\u76d6\uff0c\u800c\u643a\u5e26HER2\u6269\u589e\u6216\u8005MET\u6269\u589e\u7684\u975e\u5c0f\u7ec6\u80de\u80ba\u764c\u60a3\u8005\u5206\u522b\u5360\u5230\u4e86\u6574\u4f53\u4eba\u7fa4\u76841-3%\uff0c\u82e5\u4f7f\u7528PCR\u6280\u672f\u53ef\u80fd\u5bfc\u81f4\u8fd9\u4e9b\u53ef\u7528\u836f\u7684\u9a71\u52a8\u57fa\u56e0\u7a81\u53d8\u7684\u60a3\u8005\u4f1a\u88ab\u9057\u6f0f\u3002<\/p>\n<\/div>
<\/span><\/div><\/div><\/div>\u5404\u4f4d\u53ef\u901a\u8fc7\u68c0\u67e5\u81ea\u5df1\u62ff\u5230\u7684\u533b\u9662\u7684\u68c0\u6d4b\u62a5\u544a\u4e0a\u68c0\u6d4b\u6280\u672f\u680f\u662f\u5426\u6709\u201c\u8367\u5149\u5b9a\u91cfPCR\u201d\u3001\u201cRT-PCR\u201d\u4ee5\u53ca\u201cARMS\u201d\u7b49\u5b57\u6837\uff0c\u82e5\u6709\u7684\u8bdd\u5219\u4e3aPCR\u6280\u672f\u3002\u5982\u679c\u5404\u4f4d\u4e00\u6b65\u4e00\u6b65\u6392\u67e5\u81ea\u8eab\u68c0\u6d4b\u6d41\u7a0b\uff0c\u786e\u8ba4\u81ea\u8eab\u764c\u79cd\u6709\u9776\u5411\u836f\u4e14\u4f7f\u7528\u4e86\u6b63\u786e\u5408\u683c\u7684\u7ec4\u7ec7\u6837\u672c\uff0c\u6700\u540e\u4f7f\u7528PCR\u6280\u672f\u68c0\u6d4b\u4e3a\u9634\u6027\uff0c\u90a3\u4e48\u6709\u6761\u4ef6\u7684\u5bb6\u5ead\u5728\u5269\u4f59\u6837\u672c\u5145\u8db3\u4ee5\u53ca\u7ecf\u6d4e\u5141\u8bb8\u7684\u60c5\u51b5\u4e0b\uff0c\u53ef\u4ee5\u8003\u8651\u518d\u5c1d\u8bd5NGS\u68c0\u6d4b\uff0c\u770b\u81ea\u8eab\u662f\u5426\u5c5e\u4e8e\u4e0a\u8ff0\u5c0f\u6982\u7387\u7f55\u89c1\u7a81\u53d8\u7c7b\u578b\u3002<\/p>\n<\/div>
\u603b\u7ed3<\/div><\/h4><\/div>\u6700\u540e\u5e2e\u5404\u4f4d\u68b3\u7406\u4e00\u4e0b\u82e5\u57fa\u56e0\u68c0\u6d4b\u62a5\u544a\u9634\u6027\uff0c\u4ece\u57fa\u56e0\u68c0\u6d4b\u89d2\u5ea6\uff0c\u8fd8\u53ef\u4ee5\u505a\u54ea\u4e9b\u5c1d\u8bd5\uff1a<\/p>\n
1. \u56de\u5f52\u75c5\u7406\u62a5\u544a\uff0c\u786e\u8ba4\u81ea\u8eab\u764c\u79cd\uff0c\u4e86\u89e3\u81ea\u8eab\u764c\u79cd\u662f\u5426\u6709\u5bf9\u5e94\u9776\u5411\u836f\u4ee5\u53ca\u9776\u5411\u57fa\u56e0\u7a81\u53d8\u53ef\u80fd\u6027\u5927\u5c0f\uff0c\u82e5\u65e0\u76f8\u5173\u9776\u5411\u836f\uff0c\u5219\u518d\u6b21\u57fa\u56e0\u68c0\u6d4b\u610f\u4e49\u4e0d\u5927\uff1b<\/p>\n
2. \u56de\u987e\u68c0\u6d4b\u4f7f\u7528\u7684\u6837\u672c\u7c7b\u578b\uff0c\u82e5\u4ec5\u4f7f\u7528\u8840\u6db2\u6837\u672c\u4e14\u672a\u68c0\u6d4b\u5230\u4efb\u4f55\u7a81\u53d8\uff0c\u5219\u6709\u6761\u4ef6\u7684\u60c5\u51b5\u4e0b\u53ef\u8003\u8651\u5c3d\u91cf\u83b7\u53d6\u7ec4\u7ec7\u6837\u672c\uff0c\u5728\u786e\u5b9a\u764c\u79cd\u7c7b\u578b\u9002\u5408\u540e\u7528\u7ec4\u7ec7\u6837\u672c\u518d\u884c\u68c0\u6d4b\uff1b<\/p>\n
3. \u56de\u987e\u68c0\u6d4b\u4f7f\u7528\u7684\u6837\u672c\u7c7b\u578b\uff0c\u82e5\u4f7f\u7528\u7684\u4e3a\u65b0\u9c9c\u6807\u672c\u6216\u6837\u672c\u91cf\u4e0d\u8db3\u7684\u77f3\u8721\u5207\u7247\uff0c\u5219\u4ecd\u662f\u56de\u5f52\u75c5\u7406\u62a5\u544a\uff0c\u5148\u786e\u8ba4\u6b64\u6b21\u53d6\u6837\u662f\u5426\u53d6\u5230\u80bf\u7624\u4ee5\u53ca\u80bf\u7624\u542b\u91cf\u662f\u5426\u8fbe\u6807\uff0c\u82e5\u4e0d\u7b26\u5408\u5219\u53ef\u8003\u8651\u91cd\u65b0\u53d6\u6837\uff0c\u8fbe\u6807\u540e\u518d\u9001\u68c0\uff0c\u82e5\u7b26\u5408\u6807\u51c6\u5219\u7ed3\u679c\u57fa\u672c\u53ef\u4fe1\uff0c\u518d\u884c\u68c0\u6d4b\u6539\u53d8\u53ef\u80fd\u6027\u4e0d\u5927\uff1b<\/p>\n
4. \u5ba1\u89c6\u68c0\u6d4b\u6280\u672f\uff0c\u82e5\u764c\u79cd\u3001\u6837\u672c\u5747\u65e0\u95ee\u9898\uff0c\u4f7f\u7528\u7684\u662fPCR\u6280\u672f\u68c0\u6d4b\uff0c\u7ecf\u6d4e\u5141\u8bb8\u4e14\u5269\u4f59\u6837\u672c\u8db3\u591f\u7684\u60c5\u51b5\u4e0b\u53ef\u8003\u8651\u518d\u884cNGS\u6280\u672f\u68c0\u6d4b\uff0c\u4ee5\u514d\u9057\u6f0f\u5c0f\u6982\u7387\u7684\u7f55\u89c1\u7a81\u53d8\u4f4d\u70b9\u53ca\u7c7b\u578b\u3002<\/p>\n
5. \u82e5\u764c\u79cd\u3001\u6837\u672c\uff0c\u8fd8\u6709\u68c0\u6d4b\u6280\u672f\u5747\u65e0\u95ee\u9898\uff0c\u90a3\u4e48\u57fa\u56e0\u68c0\u6d4b\u7ed3\u679c\u95ee\u9898\u4e0d\u5927\uff0c\u57fa\u672c\u4e0d\u4f1a\u6709\u9519\u6f0f\uff0c\u77ed\u671f\u5185\u4e0d\u9700\u8981\u4e8c\u6b21\u68c0\u6d4b\uff0c\u6539\u53d8\u53ef\u80fd\u6027\u4e5f\u4e0d\u9ad8\uff0c\u5b9e\u5728\u60f3\u640f\u4e00\u640f\u5efa\u8bae\u5728\u63a5\u53d7\u6b63\u89c4\u6cbb\u7597\u51fa\u73b0\u8fdb\u5c55\uff0c\u60c5\u51b5\u6709\u53d8\u5316\u4e4b\u540e\u624d\u8003\u8651\u3002<\/p>\n
\u672c\u6587\u7ae0\u8f6c\u8f7d\u4e8e\uff1a \u4e0e\u7231\u5171\u821e\u8ba2\u9605\u53f7<\/p>\n
\u539f\u6587\u94fe\u63a5\uff1ahttps:\/\/mp.weixin.qq.com\/s\/w6yaj-CImt27KZFIDDHHQw<\/p>\n<\/div><\/div><\/div><\/div><\/div><\/p>","protected":false},"excerpt":{"rendered":"
Patients who have just been diagnosed with non-small cell lung cancer have raised a common question: \"What should I do if the first genetic test shows no mutation? Do I need to retest?\" Regarding this issue, I would like to propose the following ideas from the perspective of genetic testing for your reference.<\/p>","protected":false},"author":1,"featured_media":3606,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"content-type":"","om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[202],"tags":[],"class_list":["post-1323","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-202"],"acf":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/posts\/1323","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/comments?post=1323"}],"version-history":[{"count":6,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/posts\/1323\/revisions"}],"predecessor-version":[{"id":3618,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/posts\/1323\/revisions\/3618"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/media\/3606"}],"wp:attachment":[{"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/media?parent=1323"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/categories?post=1323"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/tags?post=1323"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
\u5f88\u591a\u5584\u4e8e\u5b66\u4e60\u7684\u75c5\u53cb\u5bf9\u4e8e\u68c0\u6d4b\u6280\u672f\u4e5f\u6709\u6240\u4e86\u89e3\uff0c\u5bf9\u4e8ePCR\u548cNGS\u6280\u672f\u5e76\u4e0d\u964c\u751f\uff0c\u6211\u4e4b\u524d\u4e5f\u5199\u8fc7\u300a\u9662\u5185\u9662\u5916\u57fa\u56e0\u68c0\u6d4b\u7684\u533a\u522b\u300b\uff08\u70b9\u51fb\u9605\u8bfb\uff09\uff0c\u5927\u90e8\u5206\u533b\u9662\u9662\u5185\u5747\u91c7\u7528PCR\u6280\u672f\uff0c\u65b9\u4fbf\u5feb\u6377\u4e14\u57fa\u672c\u80fd\u591f\u8986\u76d6\u9664NTRK\u4ee5\u5916\u7684\u5176\u4ed6\u9776\u5411\u57fa\u56e0\uff0c\u53ef\u4ee5\u6ee1\u8db3\u5927\u591a\u6570\u60a3\u8005\u7684\u68c0\u6d4b\u9700\u6c42\uff0c\u4e0d\u8fc7PCR\u6280\u672f\u5b58\u5728\u4e00\u5b9a\u7f3a\u9677\uff0c\u53ef\u80fd\u9020\u6210\u6781\u5c0f\u6982\u7387\u9057\u6f0f\u3002<\/p>\n<\/div>
\n1.PCR\u6280\u672f\u96be\u4ee5\u8986\u76d6\u9776\u5411\u57fa\u56e0\u7684\u7f55\u89c1\u7a81\u53d8\u578b<\/p>\n<\/div><\/h4><\/div>
\u901a\u4fd7\u4e00\u70b9\u8bf4\uff0cPCR\u6280\u672f\u5c31\u50cf\u662f\u62ff\u7740\u73b0\u6210\u7684\u6863\u6848\u53bb\u6392\u67e5\u5acc\u7591\u4eba\uff0c\u800cNGS\u6280\u672f\u5219\u662f\u5730\u6bef\u5f0f\u641c\u67e5\uff0c\u65e0\u8bba\u662f\u6863\u6848\u91cc\u7684\u8fd8\u662f\u6863\u6848\u5916\u7684\uff0c\u53ea\u8981\u68c0\u67e5\u53d1\u73b0\u6709\u95ee\u9898\u7684\u901a\u901a\u4e0a\u62a5\u3002PCR\u6280\u672f\u53ef\u7528\u4e8e\u68c0\u6d4b\u70b9\u7a81\u53d8\u4e0e\u878d\u5408\u7a81\u53d8\uff0c\u53ef\u8986\u76d6\u76ee\u6807\u57fa\u56e0\u7684\u5404\u79cd\u5df2\u77e5\u7a81\u53d8\u7c7b\u578b\uff0c\u4f46\u5bf9\u4e8e\u672a\u77e5\u53ca\u7f55\u89c1\u7a81\u53d8\u4f4d\u70b9\u5219\u7565\u663e\u65e0\u529b\u3002<\/p>\n
\u4ee5\u6700\u5e38\u89c1\u7684EGFR\u57fa\u56e0\u68c0\u6d4b\u4e3a\u4f8b\uff0c\u76ee\u524d\u56fd\u5185\u5df2\u83b7\u6279\u7684PCR\u8bd5\u5242\u76d2\u7684\u68c0\u6d4b\u4f4d\u70b9\u4e3b\u8981\u5305\u62ec\u5404\u7c7bEGFR 19\u7f3a\u5931\u7a81\u53d8\u3001EGFR L858R\u7a81\u53d8\u3001EGFR T790M\u7a81\u53d8\uff0cEGFR 20\u63d2\u5165\u4ee5\u53ca\u90e8\u5206\u4f4d\u70b9\u3002<\/p>\n
\u867d\u7136\u8fd9\u4e9b\u5df2\u7ecf\u8986\u76d6\u4e86\u8d85\u8fc790%\u7684EGFR\u7a81\u53d8\uff0c\u4f46EGFR\u57fa\u56e0\u768418\u300119\u300120\u300121\u5916\u663e\u5b50\u5185\u4ecd\u53ef\u80fd\u51fa\u73b0\u5982EGFR C797S\u3001EGFR L833V\u3001EGFR L747Q\u7b49\u7b49\u66f4\u52a0\u5c11\u89c1\u7684\u7a81\u53d8\u4f4d\u70b9\u3002\u8fd9\u4e9b\u7a81\u53d8\u6216\u51fa\u73b0\u7684\u6982\u7387\u6781\u4f4e\uff08\u5373\u7f55\u89c1\u7a81\u53d8\u4f4d\u70b9\uff09\u6216\u7279\u6b8a\u60c5\u51b5\u4e0b\u53ef\u80fd\u51fa\u73b0\uff0c\u53ef\u80fd\u4ec5\u5360\u6240\u6709EGFR\u7a81\u53d8\u76841-5%\u5de6\u53f3\uff08\u8f83\u96be\u6709\u5b8c\u5168\u7edf\u8ba1\uff09\uff0c\u5bf9\u4e8e\u8fd9\u4e9b\u4e0d\u5728\u68c0\u6d4b\u8303\u56f4\u5185\u7684\u7a81\u53d8\u4f4d\u70b9\uff0cPCR\u6280\u672f\u65e0\u80fd\u4e3a\u529b\uff0c\u4f46NGS\u6280\u672f\u5219\u6709\u53ef\u80fd\u68c0\u51fa\u3002<\/p>\n<\/div>
<\/span><\/div>\u518d\u4ee5\u88ab\u79f0\u4e3a\u201c\u94bb\u77f3\u7a81\u53d8\u201d\u7684ALK\u878d\u5408\u7a81\u53d8\u4e3a\u4f8b\uff0c\u8be5\u7a81\u53d8\u7c7b\u578b\u4e0d\u540c\u4e8e\u4e0a\u8ff0\u7684EGFR\u70b9\u7a81\u53d8\uff0c\u5e76\u4e0d\u662f\u5355\u4e2a\u57fa\u56e0\u7684\u4e8b\uff0c\u5b83\u662f\u4e24\u4e2a\u57fa\u56e0\u95f4\u7684\u65ad\u88c2\u4e0e\u878d\u5408\uff0c\u6211\u4eec\u5c06\u8fd9\u79cd\u878d\u5408\u7684\u4e24\u4e2a\u57fa\u56e0\u6210\u4e3a\u201c\u878d\u5408\u4f34\u4fa3\u201d\uff0cALK\u878d\u5408\u5373\u662f\u6307\u5176\u4e2d\u4e00\u4e2a\u4f34\u4fa3\u56fa\u5b9a\u4e3aALK\u57fa\u56e0\uff0c\u53e6\u4e00\u534a\u53ef\u80fd\u4f1a\u6709\u591a\u79cd\u3002<\/p>\n
\u6700\u5e38\u89c1\u7684ALK\u878d\u5408\u53d1\u751f\u5728EML4\u4e0eALK\u57fa\u56e0\u4e4b\u95f4\uff0c\u4f46\u968f\u7740\u7814\u7a76\u53d1\u5c55\uff0c\u6211\u4eec\u53d1\u73b0\u4e86\u66f4\u591a\u4f34\u4fa3\u57fa\u56e0\uff0c\u8f83\u65e9\u4ee5\u524d\u7684PCR\u8bd5\u5242\u76d2\u4ec5\u53ef\u68c0\u6d4bEML4-ALK\u878d\u5408\u7c7b\u578b\uff0c\u5176\u4ed6\u878d\u5408\u4f34\u4fa3\u5f62\u6210\u7684ALK\u878d\u5408\u5219\u65e0\u6cd5\u53d1\u73b0\uff0c\u73b0\u5728\u6709\u4e9b\u66f4\u65b0\u7684PCR\u8bd5\u5242\u76d2\u9664EML4\u57fa\u56e0\u4ee5\u5916\uff0c\u8fd8\u5305\u542bKIF5B\u3001KCL1\u3001TFG\u7b49\u6982\u7387\u76f8\u5bf9\u8f83\u4f4e\u7684\u878d\u5408\u4f34\u4fa3\uff0c\u8fdb\u4e00\u6b65\u6269\u5927\u4e86PCR\u6280\u672f\u53ef\u68c0\u6d4b\u5230\u7684ALK\u878d\u5408\u7c7b\u578b\u3002<\/p>\n
\u53ef\u4ee5\u8bf490%\u751a\u81f395%\u4ee5\u4e0a\u7684ALK\u878d\u5408\u60a3\u8005\u90fd\u53ef\u901a\u8fc7PCR\u6280\u672f\u53d1\u73b0\uff0c\u4f46ALK\u57fa\u56e0\u7684\u878d\u5408\u4f34\u4fa3\u4ecd\u7136\u8d85\u51fa\u6b64\u8303\u56f4\uff0c\u4f8b\u5982\u6211\u66fe\u9047\u5230\u8fc7\u67d0\u4e2a\u75c5\u53cb\u662fDIRC3-ALK\u878d\u5408\uff0cNGS\u6280\u672f\u7684\u7279\u6027\u8ba9\u5b83\u4e0d\u53d7\u8fd9\u79cd\u8303\u56f4\u9650\u5236\uff0c\u65e0\u8bba\u54ea\u79cd\u878d\u5408\u4f34\u4fa3\uff0c\u7406\u8bba\u4e0a\u90fd\u53ef\u4ee5\u68c0\u51fa\uff0c\u907f\u514d\u8fd9\u79cd\u5c0f\u6982\u7387\u610f\u5916\u3002<\/p>\n<\/div>
<\/span><\/div>\n2. PCR\u6280\u672f\u80fd\u4ee5\u8986\u76d6\u90e8\u5206\u7a81\u53d8\u7c7b\u578b<\/p>\n<\/div><\/h4><\/div>
PCR\u6280\u672f\u5bf9\u4e8e\u68c0\u6d4b\u70b9\u7a81\u53d8\u3001\u878d\u5408\u7a81\u53d8\u7684\u68c0\u6d4b\u80fd\u529b\u8f83\u5f3a\uff0c\u4f46\u76ee\u524d\u5404\u533b\u9662\u5e38\u7528\u7684PCR\u68c0\u6d4b\u8bd5\u5242\u76d2\u5bf9\u4e8e\u201c\u6269\u589e\u201d\u8fd9\u4e00\u7a81\u53d8\u7c7b\u578b\u57fa\u672c\u6ca1\u6709\u8986\u76d6\uff0c\u800c\u643a\u5e26HER2\u6269\u589e\u6216\u8005MET\u6269\u589e\u7684\u975e\u5c0f\u7ec6\u80de\u80ba\u764c\u60a3\u8005\u5206\u522b\u5360\u5230\u4e86\u6574\u4f53\u4eba\u7fa4\u76841-3%\uff0c\u82e5\u4f7f\u7528PCR\u6280\u672f\u53ef\u80fd\u5bfc\u81f4\u8fd9\u4e9b\u53ef\u7528\u836f\u7684\u9a71\u52a8\u57fa\u56e0\u7a81\u53d8\u7684\u60a3\u8005\u4f1a\u88ab\u9057\u6f0f\u3002<\/p>\n<\/div>
<\/span><\/div><\/div><\/div>\u5404\u4f4d\u53ef\u901a\u8fc7\u68c0\u67e5\u81ea\u5df1\u62ff\u5230\u7684\u533b\u9662\u7684\u68c0\u6d4b\u62a5\u544a\u4e0a\u68c0\u6d4b\u6280\u672f\u680f\u662f\u5426\u6709\u201c\u8367\u5149\u5b9a\u91cfPCR\u201d\u3001\u201cRT-PCR\u201d\u4ee5\u53ca\u201cARMS\u201d\u7b49\u5b57\u6837\uff0c\u82e5\u6709\u7684\u8bdd\u5219\u4e3aPCR\u6280\u672f\u3002\u5982\u679c\u5404\u4f4d\u4e00\u6b65\u4e00\u6b65\u6392\u67e5\u81ea\u8eab\u68c0\u6d4b\u6d41\u7a0b\uff0c\u786e\u8ba4\u81ea\u8eab\u764c\u79cd\u6709\u9776\u5411\u836f\u4e14\u4f7f\u7528\u4e86\u6b63\u786e\u5408\u683c\u7684\u7ec4\u7ec7\u6837\u672c\uff0c\u6700\u540e\u4f7f\u7528PCR\u6280\u672f\u68c0\u6d4b\u4e3a\u9634\u6027\uff0c\u90a3\u4e48\u6709\u6761\u4ef6\u7684\u5bb6\u5ead\u5728\u5269\u4f59\u6837\u672c\u5145\u8db3\u4ee5\u53ca\u7ecf\u6d4e\u5141\u8bb8\u7684\u60c5\u51b5\u4e0b\uff0c\u53ef\u4ee5\u8003\u8651\u518d\u5c1d\u8bd5NGS\u68c0\u6d4b\uff0c\u770b\u81ea\u8eab\u662f\u5426\u5c5e\u4e8e\u4e0a\u8ff0\u5c0f\u6982\u7387\u7f55\u89c1\u7a81\u53d8\u7c7b\u578b\u3002<\/p>\n<\/div>
\u603b\u7ed3<\/div><\/h4><\/div>\u6700\u540e\u5e2e\u5404\u4f4d\u68b3\u7406\u4e00\u4e0b\u82e5\u57fa\u56e0\u68c0\u6d4b\u62a5\u544a\u9634\u6027\uff0c\u4ece\u57fa\u56e0\u68c0\u6d4b\u89d2\u5ea6\uff0c\u8fd8\u53ef\u4ee5\u505a\u54ea\u4e9b\u5c1d\u8bd5\uff1a<\/p>\n
1. \u56de\u5f52\u75c5\u7406\u62a5\u544a\uff0c\u786e\u8ba4\u81ea\u8eab\u764c\u79cd\uff0c\u4e86\u89e3\u81ea\u8eab\u764c\u79cd\u662f\u5426\u6709\u5bf9\u5e94\u9776\u5411\u836f\u4ee5\u53ca\u9776\u5411\u57fa\u56e0\u7a81\u53d8\u53ef\u80fd\u6027\u5927\u5c0f\uff0c\u82e5\u65e0\u76f8\u5173\u9776\u5411\u836f\uff0c\u5219\u518d\u6b21\u57fa\u56e0\u68c0\u6d4b\u610f\u4e49\u4e0d\u5927\uff1b<\/p>\n
2. \u56de\u987e\u68c0\u6d4b\u4f7f\u7528\u7684\u6837\u672c\u7c7b\u578b\uff0c\u82e5\u4ec5\u4f7f\u7528\u8840\u6db2\u6837\u672c\u4e14\u672a\u68c0\u6d4b\u5230\u4efb\u4f55\u7a81\u53d8\uff0c\u5219\u6709\u6761\u4ef6\u7684\u60c5\u51b5\u4e0b\u53ef\u8003\u8651\u5c3d\u91cf\u83b7\u53d6\u7ec4\u7ec7\u6837\u672c\uff0c\u5728\u786e\u5b9a\u764c\u79cd\u7c7b\u578b\u9002\u5408\u540e\u7528\u7ec4\u7ec7\u6837\u672c\u518d\u884c\u68c0\u6d4b\uff1b<\/p>\n
3. \u56de\u987e\u68c0\u6d4b\u4f7f\u7528\u7684\u6837\u672c\u7c7b\u578b\uff0c\u82e5\u4f7f\u7528\u7684\u4e3a\u65b0\u9c9c\u6807\u672c\u6216\u6837\u672c\u91cf\u4e0d\u8db3\u7684\u77f3\u8721\u5207\u7247\uff0c\u5219\u4ecd\u662f\u56de\u5f52\u75c5\u7406\u62a5\u544a\uff0c\u5148\u786e\u8ba4\u6b64\u6b21\u53d6\u6837\u662f\u5426\u53d6\u5230\u80bf\u7624\u4ee5\u53ca\u80bf\u7624\u542b\u91cf\u662f\u5426\u8fbe\u6807\uff0c\u82e5\u4e0d\u7b26\u5408\u5219\u53ef\u8003\u8651\u91cd\u65b0\u53d6\u6837\uff0c\u8fbe\u6807\u540e\u518d\u9001\u68c0\uff0c\u82e5\u7b26\u5408\u6807\u51c6\u5219\u7ed3\u679c\u57fa\u672c\u53ef\u4fe1\uff0c\u518d\u884c\u68c0\u6d4b\u6539\u53d8\u53ef\u80fd\u6027\u4e0d\u5927\uff1b<\/p>\n
4. \u5ba1\u89c6\u68c0\u6d4b\u6280\u672f\uff0c\u82e5\u764c\u79cd\u3001\u6837\u672c\u5747\u65e0\u95ee\u9898\uff0c\u4f7f\u7528\u7684\u662fPCR\u6280\u672f\u68c0\u6d4b\uff0c\u7ecf\u6d4e\u5141\u8bb8\u4e14\u5269\u4f59\u6837\u672c\u8db3\u591f\u7684\u60c5\u51b5\u4e0b\u53ef\u8003\u8651\u518d\u884cNGS\u6280\u672f\u68c0\u6d4b\uff0c\u4ee5\u514d\u9057\u6f0f\u5c0f\u6982\u7387\u7684\u7f55\u89c1\u7a81\u53d8\u4f4d\u70b9\u53ca\u7c7b\u578b\u3002<\/p>\n
5. \u82e5\u764c\u79cd\u3001\u6837\u672c\uff0c\u8fd8\u6709\u68c0\u6d4b\u6280\u672f\u5747\u65e0\u95ee\u9898\uff0c\u90a3\u4e48\u57fa\u56e0\u68c0\u6d4b\u7ed3\u679c\u95ee\u9898\u4e0d\u5927\uff0c\u57fa\u672c\u4e0d\u4f1a\u6709\u9519\u6f0f\uff0c\u77ed\u671f\u5185\u4e0d\u9700\u8981\u4e8c\u6b21\u68c0\u6d4b\uff0c\u6539\u53d8\u53ef\u80fd\u6027\u4e5f\u4e0d\u9ad8\uff0c\u5b9e\u5728\u60f3\u640f\u4e00\u640f\u5efa\u8bae\u5728\u63a5\u53d7\u6b63\u89c4\u6cbb\u7597\u51fa\u73b0\u8fdb\u5c55\uff0c\u60c5\u51b5\u6709\u53d8\u5316\u4e4b\u540e\u624d\u8003\u8651\u3002<\/p>\n
\u672c\u6587\u7ae0\u8f6c\u8f7d\u4e8e\uff1a \u4e0e\u7231\u5171\u821e\u8ba2\u9605\u53f7<\/p>\n
\u539f\u6587\u94fe\u63a5\uff1ahttps:\/\/mp.weixin.qq.com\/s\/w6yaj-CImt27KZFIDDHHQw<\/p>\n<\/div><\/div><\/div><\/div><\/div><\/p>","protected":false},"excerpt":{"rendered":"
Patients who have just been diagnosed with non-small cell lung cancer have raised a common question: \"What should I do if the first genetic test shows no mutation? Do I need to retest?\" Regarding this issue, I would like to propose the following ideas from the perspective of genetic testing for your reference.<\/p>","protected":false},"author":1,"featured_media":3606,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"content-type":"","om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[202],"tags":[],"class_list":["post-1323","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-202"],"acf":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/posts\/1323","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/comments?post=1323"}],"version-history":[{"count":6,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/posts\/1323\/revisions"}],"predecessor-version":[{"id":3618,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/posts\/1323\/revisions\/3618"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/media\/3606"}],"wp:attachment":[{"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/media?parent=1323"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/categories?post=1323"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/tags?post=1323"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
1.PCR\u6280\u672f\u96be\u4ee5\u8986\u76d6\u9776\u5411\u57fa\u56e0\u7684\u7f55\u89c1\u7a81\u53d8\u578b<\/p>\n<\/div><\/h4><\/div>
\u901a\u4fd7\u4e00\u70b9\u8bf4\uff0cPCR\u6280\u672f\u5c31\u50cf\u662f\u62ff\u7740\u73b0\u6210\u7684\u6863\u6848\u53bb\u6392\u67e5\u5acc\u7591\u4eba\uff0c\u800cNGS\u6280\u672f\u5219\u662f\u5730\u6bef\u5f0f\u641c\u67e5\uff0c\u65e0\u8bba\u662f\u6863\u6848\u91cc\u7684\u8fd8\u662f\u6863\u6848\u5916\u7684\uff0c\u53ea\u8981\u68c0\u67e5\u53d1\u73b0\u6709\u95ee\u9898\u7684\u901a\u901a\u4e0a\u62a5\u3002PCR\u6280\u672f\u53ef\u7528\u4e8e\u68c0\u6d4b\u70b9\u7a81\u53d8\u4e0e\u878d\u5408\u7a81\u53d8\uff0c\u53ef\u8986\u76d6\u76ee\u6807\u57fa\u56e0\u7684\u5404\u79cd\u5df2\u77e5\u7a81\u53d8\u7c7b\u578b\uff0c\u4f46\u5bf9\u4e8e\u672a\u77e5\u53ca\u7f55\u89c1\u7a81\u53d8\u4f4d\u70b9\u5219\u7565\u663e\u65e0\u529b\u3002<\/p>\n
\u4ee5\u6700\u5e38\u89c1\u7684EGFR\u57fa\u56e0\u68c0\u6d4b\u4e3a\u4f8b\uff0c\u76ee\u524d\u56fd\u5185\u5df2\u83b7\u6279\u7684PCR\u8bd5\u5242\u76d2\u7684\u68c0\u6d4b\u4f4d\u70b9\u4e3b\u8981\u5305\u62ec\u5404\u7c7bEGFR 19\u7f3a\u5931\u7a81\u53d8\u3001EGFR L858R\u7a81\u53d8\u3001EGFR T790M\u7a81\u53d8\uff0cEGFR 20\u63d2\u5165\u4ee5\u53ca\u90e8\u5206\u4f4d\u70b9\u3002<\/p>\n
\u867d\u7136\u8fd9\u4e9b\u5df2\u7ecf\u8986\u76d6\u4e86\u8d85\u8fc790%\u7684EGFR\u7a81\u53d8\uff0c\u4f46EGFR\u57fa\u56e0\u768418\u300119\u300120\u300121\u5916\u663e\u5b50\u5185\u4ecd\u53ef\u80fd\u51fa\u73b0\u5982EGFR C797S\u3001EGFR L833V\u3001EGFR L747Q\u7b49\u7b49\u66f4\u52a0\u5c11\u89c1\u7684\u7a81\u53d8\u4f4d\u70b9\u3002\u8fd9\u4e9b\u7a81\u53d8\u6216\u51fa\u73b0\u7684\u6982\u7387\u6781\u4f4e\uff08\u5373\u7f55\u89c1\u7a81\u53d8\u4f4d\u70b9\uff09\u6216\u7279\u6b8a\u60c5\u51b5\u4e0b\u53ef\u80fd\u51fa\u73b0\uff0c\u53ef\u80fd\u4ec5\u5360\u6240\u6709EGFR\u7a81\u53d8\u76841-5%\u5de6\u53f3\uff08\u8f83\u96be\u6709\u5b8c\u5168\u7edf\u8ba1\uff09\uff0c\u5bf9\u4e8e\u8fd9\u4e9b\u4e0d\u5728\u68c0\u6d4b\u8303\u56f4\u5185\u7684\u7a81\u53d8\u4f4d\u70b9\uff0cPCR\u6280\u672f\u65e0\u80fd\u4e3a\u529b\uff0c\u4f46NGS\u6280\u672f\u5219\u6709\u53ef\u80fd\u68c0\u51fa\u3002<\/p>\n<\/div>
<\/span><\/div>\u518d\u4ee5\u88ab\u79f0\u4e3a\u201c\u94bb\u77f3\u7a81\u53d8\u201d\u7684ALK\u878d\u5408\u7a81\u53d8\u4e3a\u4f8b\uff0c\u8be5\u7a81\u53d8\u7c7b\u578b\u4e0d\u540c\u4e8e\u4e0a\u8ff0\u7684EGFR\u70b9\u7a81\u53d8\uff0c\u5e76\u4e0d\u662f\u5355\u4e2a\u57fa\u56e0\u7684\u4e8b\uff0c\u5b83\u662f\u4e24\u4e2a\u57fa\u56e0\u95f4\u7684\u65ad\u88c2\u4e0e\u878d\u5408\uff0c\u6211\u4eec\u5c06\u8fd9\u79cd\u878d\u5408\u7684\u4e24\u4e2a\u57fa\u56e0\u6210\u4e3a\u201c\u878d\u5408\u4f34\u4fa3\u201d\uff0cALK\u878d\u5408\u5373\u662f\u6307\u5176\u4e2d\u4e00\u4e2a\u4f34\u4fa3\u56fa\u5b9a\u4e3aALK\u57fa\u56e0\uff0c\u53e6\u4e00\u534a\u53ef\u80fd\u4f1a\u6709\u591a\u79cd\u3002<\/p>\n
\u6700\u5e38\u89c1\u7684ALK\u878d\u5408\u53d1\u751f\u5728EML4\u4e0eALK\u57fa\u56e0\u4e4b\u95f4\uff0c\u4f46\u968f\u7740\u7814\u7a76\u53d1\u5c55\uff0c\u6211\u4eec\u53d1\u73b0\u4e86\u66f4\u591a\u4f34\u4fa3\u57fa\u56e0\uff0c\u8f83\u65e9\u4ee5\u524d\u7684PCR\u8bd5\u5242\u76d2\u4ec5\u53ef\u68c0\u6d4bEML4-ALK\u878d\u5408\u7c7b\u578b\uff0c\u5176\u4ed6\u878d\u5408\u4f34\u4fa3\u5f62\u6210\u7684ALK\u878d\u5408\u5219\u65e0\u6cd5\u53d1\u73b0\uff0c\u73b0\u5728\u6709\u4e9b\u66f4\u65b0\u7684PCR\u8bd5\u5242\u76d2\u9664EML4\u57fa\u56e0\u4ee5\u5916\uff0c\u8fd8\u5305\u542bKIF5B\u3001KCL1\u3001TFG\u7b49\u6982\u7387\u76f8\u5bf9\u8f83\u4f4e\u7684\u878d\u5408\u4f34\u4fa3\uff0c\u8fdb\u4e00\u6b65\u6269\u5927\u4e86PCR\u6280\u672f\u53ef\u68c0\u6d4b\u5230\u7684ALK\u878d\u5408\u7c7b\u578b\u3002<\/p>\n
\u53ef\u4ee5\u8bf490%\u751a\u81f395%\u4ee5\u4e0a\u7684ALK\u878d\u5408\u60a3\u8005\u90fd\u53ef\u901a\u8fc7PCR\u6280\u672f\u53d1\u73b0\uff0c\u4f46ALK\u57fa\u56e0\u7684\u878d\u5408\u4f34\u4fa3\u4ecd\u7136\u8d85\u51fa\u6b64\u8303\u56f4\uff0c\u4f8b\u5982\u6211\u66fe\u9047\u5230\u8fc7\u67d0\u4e2a\u75c5\u53cb\u662fDIRC3-ALK\u878d\u5408\uff0cNGS\u6280\u672f\u7684\u7279\u6027\u8ba9\u5b83\u4e0d\u53d7\u8fd9\u79cd\u8303\u56f4\u9650\u5236\uff0c\u65e0\u8bba\u54ea\u79cd\u878d\u5408\u4f34\u4fa3\uff0c\u7406\u8bba\u4e0a\u90fd\u53ef\u4ee5\u68c0\u51fa\uff0c\u907f\u514d\u8fd9\u79cd\u5c0f\u6982\u7387\u610f\u5916\u3002<\/p>\n<\/div>
<\/span><\/div>\n2. PCR\u6280\u672f\u80fd\u4ee5\u8986\u76d6\u90e8\u5206\u7a81\u53d8\u7c7b\u578b<\/p>\n<\/div><\/h4><\/div>
PCR\u6280\u672f\u5bf9\u4e8e\u68c0\u6d4b\u70b9\u7a81\u53d8\u3001\u878d\u5408\u7a81\u53d8\u7684\u68c0\u6d4b\u80fd\u529b\u8f83\u5f3a\uff0c\u4f46\u76ee\u524d\u5404\u533b\u9662\u5e38\u7528\u7684PCR\u68c0\u6d4b\u8bd5\u5242\u76d2\u5bf9\u4e8e\u201c\u6269\u589e\u201d\u8fd9\u4e00\u7a81\u53d8\u7c7b\u578b\u57fa\u672c\u6ca1\u6709\u8986\u76d6\uff0c\u800c\u643a\u5e26HER2\u6269\u589e\u6216\u8005MET\u6269\u589e\u7684\u975e\u5c0f\u7ec6\u80de\u80ba\u764c\u60a3\u8005\u5206\u522b\u5360\u5230\u4e86\u6574\u4f53\u4eba\u7fa4\u76841-3%\uff0c\u82e5\u4f7f\u7528PCR\u6280\u672f\u53ef\u80fd\u5bfc\u81f4\u8fd9\u4e9b\u53ef\u7528\u836f\u7684\u9a71\u52a8\u57fa\u56e0\u7a81\u53d8\u7684\u60a3\u8005\u4f1a\u88ab\u9057\u6f0f\u3002<\/p>\n<\/div>
<\/span><\/div><\/div><\/div>\u5404\u4f4d\u53ef\u901a\u8fc7\u68c0\u67e5\u81ea\u5df1\u62ff\u5230\u7684\u533b\u9662\u7684\u68c0\u6d4b\u62a5\u544a\u4e0a\u68c0\u6d4b\u6280\u672f\u680f\u662f\u5426\u6709\u201c\u8367\u5149\u5b9a\u91cfPCR\u201d\u3001\u201cRT-PCR\u201d\u4ee5\u53ca\u201cARMS\u201d\u7b49\u5b57\u6837\uff0c\u82e5\u6709\u7684\u8bdd\u5219\u4e3aPCR\u6280\u672f\u3002\u5982\u679c\u5404\u4f4d\u4e00\u6b65\u4e00\u6b65\u6392\u67e5\u81ea\u8eab\u68c0\u6d4b\u6d41\u7a0b\uff0c\u786e\u8ba4\u81ea\u8eab\u764c\u79cd\u6709\u9776\u5411\u836f\u4e14\u4f7f\u7528\u4e86\u6b63\u786e\u5408\u683c\u7684\u7ec4\u7ec7\u6837\u672c\uff0c\u6700\u540e\u4f7f\u7528PCR\u6280\u672f\u68c0\u6d4b\u4e3a\u9634\u6027\uff0c\u90a3\u4e48\u6709\u6761\u4ef6\u7684\u5bb6\u5ead\u5728\u5269\u4f59\u6837\u672c\u5145\u8db3\u4ee5\u53ca\u7ecf\u6d4e\u5141\u8bb8\u7684\u60c5\u51b5\u4e0b\uff0c\u53ef\u4ee5\u8003\u8651\u518d\u5c1d\u8bd5NGS\u68c0\u6d4b\uff0c\u770b\u81ea\u8eab\u662f\u5426\u5c5e\u4e8e\u4e0a\u8ff0\u5c0f\u6982\u7387\u7f55\u89c1\u7a81\u53d8\u7c7b\u578b\u3002<\/p>\n<\/div>
\u603b\u7ed3<\/div><\/h4><\/div>\u6700\u540e\u5e2e\u5404\u4f4d\u68b3\u7406\u4e00\u4e0b\u82e5\u57fa\u56e0\u68c0\u6d4b\u62a5\u544a\u9634\u6027\uff0c\u4ece\u57fa\u56e0\u68c0\u6d4b\u89d2\u5ea6\uff0c\u8fd8\u53ef\u4ee5\u505a\u54ea\u4e9b\u5c1d\u8bd5\uff1a<\/p>\n
1. \u56de\u5f52\u75c5\u7406\u62a5\u544a\uff0c\u786e\u8ba4\u81ea\u8eab\u764c\u79cd\uff0c\u4e86\u89e3\u81ea\u8eab\u764c\u79cd\u662f\u5426\u6709\u5bf9\u5e94\u9776\u5411\u836f\u4ee5\u53ca\u9776\u5411\u57fa\u56e0\u7a81\u53d8\u53ef\u80fd\u6027\u5927\u5c0f\uff0c\u82e5\u65e0\u76f8\u5173\u9776\u5411\u836f\uff0c\u5219\u518d\u6b21\u57fa\u56e0\u68c0\u6d4b\u610f\u4e49\u4e0d\u5927\uff1b<\/p>\n
2. \u56de\u987e\u68c0\u6d4b\u4f7f\u7528\u7684\u6837\u672c\u7c7b\u578b\uff0c\u82e5\u4ec5\u4f7f\u7528\u8840\u6db2\u6837\u672c\u4e14\u672a\u68c0\u6d4b\u5230\u4efb\u4f55\u7a81\u53d8\uff0c\u5219\u6709\u6761\u4ef6\u7684\u60c5\u51b5\u4e0b\u53ef\u8003\u8651\u5c3d\u91cf\u83b7\u53d6\u7ec4\u7ec7\u6837\u672c\uff0c\u5728\u786e\u5b9a\u764c\u79cd\u7c7b\u578b\u9002\u5408\u540e\u7528\u7ec4\u7ec7\u6837\u672c\u518d\u884c\u68c0\u6d4b\uff1b<\/p>\n
3. \u56de\u987e\u68c0\u6d4b\u4f7f\u7528\u7684\u6837\u672c\u7c7b\u578b\uff0c\u82e5\u4f7f\u7528\u7684\u4e3a\u65b0\u9c9c\u6807\u672c\u6216\u6837\u672c\u91cf\u4e0d\u8db3\u7684\u77f3\u8721\u5207\u7247\uff0c\u5219\u4ecd\u662f\u56de\u5f52\u75c5\u7406\u62a5\u544a\uff0c\u5148\u786e\u8ba4\u6b64\u6b21\u53d6\u6837\u662f\u5426\u53d6\u5230\u80bf\u7624\u4ee5\u53ca\u80bf\u7624\u542b\u91cf\u662f\u5426\u8fbe\u6807\uff0c\u82e5\u4e0d\u7b26\u5408\u5219\u53ef\u8003\u8651\u91cd\u65b0\u53d6\u6837\uff0c\u8fbe\u6807\u540e\u518d\u9001\u68c0\uff0c\u82e5\u7b26\u5408\u6807\u51c6\u5219\u7ed3\u679c\u57fa\u672c\u53ef\u4fe1\uff0c\u518d\u884c\u68c0\u6d4b\u6539\u53d8\u53ef\u80fd\u6027\u4e0d\u5927\uff1b<\/p>\n
4. \u5ba1\u89c6\u68c0\u6d4b\u6280\u672f\uff0c\u82e5\u764c\u79cd\u3001\u6837\u672c\u5747\u65e0\u95ee\u9898\uff0c\u4f7f\u7528\u7684\u662fPCR\u6280\u672f\u68c0\u6d4b\uff0c\u7ecf\u6d4e\u5141\u8bb8\u4e14\u5269\u4f59\u6837\u672c\u8db3\u591f\u7684\u60c5\u51b5\u4e0b\u53ef\u8003\u8651\u518d\u884cNGS\u6280\u672f\u68c0\u6d4b\uff0c\u4ee5\u514d\u9057\u6f0f\u5c0f\u6982\u7387\u7684\u7f55\u89c1\u7a81\u53d8\u4f4d\u70b9\u53ca\u7c7b\u578b\u3002<\/p>\n
5. \u82e5\u764c\u79cd\u3001\u6837\u672c\uff0c\u8fd8\u6709\u68c0\u6d4b\u6280\u672f\u5747\u65e0\u95ee\u9898\uff0c\u90a3\u4e48\u57fa\u56e0\u68c0\u6d4b\u7ed3\u679c\u95ee\u9898\u4e0d\u5927\uff0c\u57fa\u672c\u4e0d\u4f1a\u6709\u9519\u6f0f\uff0c\u77ed\u671f\u5185\u4e0d\u9700\u8981\u4e8c\u6b21\u68c0\u6d4b\uff0c\u6539\u53d8\u53ef\u80fd\u6027\u4e5f\u4e0d\u9ad8\uff0c\u5b9e\u5728\u60f3\u640f\u4e00\u640f\u5efa\u8bae\u5728\u63a5\u53d7\u6b63\u89c4\u6cbb\u7597\u51fa\u73b0\u8fdb\u5c55\uff0c\u60c5\u51b5\u6709\u53d8\u5316\u4e4b\u540e\u624d\u8003\u8651\u3002<\/p>\n
\u672c\u6587\u7ae0\u8f6c\u8f7d\u4e8e\uff1a \u4e0e\u7231\u5171\u821e\u8ba2\u9605\u53f7<\/p>\n
\u539f\u6587\u94fe\u63a5\uff1ahttps:\/\/mp.weixin.qq.com\/s\/w6yaj-CImt27KZFIDDHHQw<\/p>\n<\/div><\/div><\/div><\/div><\/div><\/p>","protected":false},"excerpt":{"rendered":"
Patients who have just been diagnosed with non-small cell lung cancer have raised a common question: \"What should I do if the first genetic test shows no mutation? Do I need to retest?\" Regarding this issue, I would like to propose the following ideas from the perspective of genetic testing for your reference.<\/p>","protected":false},"author":1,"featured_media":3606,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"content-type":"","om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[202],"tags":[],"class_list":["post-1323","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-202"],"acf":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/posts\/1323","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/comments?post=1323"}],"version-history":[{"count":6,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/posts\/1323\/revisions"}],"predecessor-version":[{"id":3618,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/posts\/1323\/revisions\/3618"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/media\/3606"}],"wp:attachment":[{"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/media?parent=1323"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/categories?post=1323"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/tags?post=1323"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
2. PCR\u6280\u672f\u80fd\u4ee5\u8986\u76d6\u90e8\u5206\u7a81\u53d8\u7c7b\u578b<\/p>\n<\/div><\/h4><\/div>
PCR\u6280\u672f\u5bf9\u4e8e\u68c0\u6d4b\u70b9\u7a81\u53d8\u3001\u878d\u5408\u7a81\u53d8\u7684\u68c0\u6d4b\u80fd\u529b\u8f83\u5f3a\uff0c\u4f46\u76ee\u524d\u5404\u533b\u9662\u5e38\u7528\u7684PCR\u68c0\u6d4b\u8bd5\u5242\u76d2\u5bf9\u4e8e\u201c\u6269\u589e\u201d\u8fd9\u4e00\u7a81\u53d8\u7c7b\u578b\u57fa\u672c\u6ca1\u6709\u8986\u76d6\uff0c\u800c\u643a\u5e26HER2\u6269\u589e\u6216\u8005MET\u6269\u589e\u7684\u975e\u5c0f\u7ec6\u80de\u80ba\u764c\u60a3\u8005\u5206\u522b\u5360\u5230\u4e86\u6574\u4f53\u4eba\u7fa4\u76841-3%\uff0c\u82e5\u4f7f\u7528PCR\u6280\u672f\u53ef\u80fd\u5bfc\u81f4\u8fd9\u4e9b\u53ef\u7528\u836f\u7684\u9a71\u52a8\u57fa\u56e0\u7a81\u53d8\u7684\u60a3\u8005\u4f1a\u88ab\u9057\u6f0f\u3002<\/p>\n<\/div>
<\/span><\/div><\/div><\/div>\u5404\u4f4d\u53ef\u901a\u8fc7\u68c0\u67e5\u81ea\u5df1\u62ff\u5230\u7684\u533b\u9662\u7684\u68c0\u6d4b\u62a5\u544a\u4e0a\u68c0\u6d4b\u6280\u672f\u680f\u662f\u5426\u6709\u201c\u8367\u5149\u5b9a\u91cfPCR\u201d\u3001\u201cRT-PCR\u201d\u4ee5\u53ca\u201cARMS\u201d\u7b49\u5b57\u6837\uff0c\u82e5\u6709\u7684\u8bdd\u5219\u4e3aPCR\u6280\u672f\u3002\u5982\u679c\u5404\u4f4d\u4e00\u6b65\u4e00\u6b65\u6392\u67e5\u81ea\u8eab\u68c0\u6d4b\u6d41\u7a0b\uff0c\u786e\u8ba4\u81ea\u8eab\u764c\u79cd\u6709\u9776\u5411\u836f\u4e14\u4f7f\u7528\u4e86\u6b63\u786e\u5408\u683c\u7684\u7ec4\u7ec7\u6837\u672c\uff0c\u6700\u540e\u4f7f\u7528PCR\u6280\u672f\u68c0\u6d4b\u4e3a\u9634\u6027\uff0c\u90a3\u4e48\u6709\u6761\u4ef6\u7684\u5bb6\u5ead\u5728\u5269\u4f59\u6837\u672c\u5145\u8db3\u4ee5\u53ca\u7ecf\u6d4e\u5141\u8bb8\u7684\u60c5\u51b5\u4e0b\uff0c\u53ef\u4ee5\u8003\u8651\u518d\u5c1d\u8bd5NGS\u68c0\u6d4b\uff0c\u770b\u81ea\u8eab\u662f\u5426\u5c5e\u4e8e\u4e0a\u8ff0\u5c0f\u6982\u7387\u7f55\u89c1\u7a81\u53d8\u7c7b\u578b\u3002<\/p>\n<\/div>
\u603b\u7ed3<\/div><\/h4><\/div>\u6700\u540e\u5e2e\u5404\u4f4d\u68b3\u7406\u4e00\u4e0b\u82e5\u57fa\u56e0\u68c0\u6d4b\u62a5\u544a\u9634\u6027\uff0c\u4ece\u57fa\u56e0\u68c0\u6d4b\u89d2\u5ea6\uff0c\u8fd8\u53ef\u4ee5\u505a\u54ea\u4e9b\u5c1d\u8bd5\uff1a<\/p>\n
1. \u56de\u5f52\u75c5\u7406\u62a5\u544a\uff0c\u786e\u8ba4\u81ea\u8eab\u764c\u79cd\uff0c\u4e86\u89e3\u81ea\u8eab\u764c\u79cd\u662f\u5426\u6709\u5bf9\u5e94\u9776\u5411\u836f\u4ee5\u53ca\u9776\u5411\u57fa\u56e0\u7a81\u53d8\u53ef\u80fd\u6027\u5927\u5c0f\uff0c\u82e5\u65e0\u76f8\u5173\u9776\u5411\u836f\uff0c\u5219\u518d\u6b21\u57fa\u56e0\u68c0\u6d4b\u610f\u4e49\u4e0d\u5927\uff1b<\/p>\n
2. \u56de\u987e\u68c0\u6d4b\u4f7f\u7528\u7684\u6837\u672c\u7c7b\u578b\uff0c\u82e5\u4ec5\u4f7f\u7528\u8840\u6db2\u6837\u672c\u4e14\u672a\u68c0\u6d4b\u5230\u4efb\u4f55\u7a81\u53d8\uff0c\u5219\u6709\u6761\u4ef6\u7684\u60c5\u51b5\u4e0b\u53ef\u8003\u8651\u5c3d\u91cf\u83b7\u53d6\u7ec4\u7ec7\u6837\u672c\uff0c\u5728\u786e\u5b9a\u764c\u79cd\u7c7b\u578b\u9002\u5408\u540e\u7528\u7ec4\u7ec7\u6837\u672c\u518d\u884c\u68c0\u6d4b\uff1b<\/p>\n
3. \u56de\u987e\u68c0\u6d4b\u4f7f\u7528\u7684\u6837\u672c\u7c7b\u578b\uff0c\u82e5\u4f7f\u7528\u7684\u4e3a\u65b0\u9c9c\u6807\u672c\u6216\u6837\u672c\u91cf\u4e0d\u8db3\u7684\u77f3\u8721\u5207\u7247\uff0c\u5219\u4ecd\u662f\u56de\u5f52\u75c5\u7406\u62a5\u544a\uff0c\u5148\u786e\u8ba4\u6b64\u6b21\u53d6\u6837\u662f\u5426\u53d6\u5230\u80bf\u7624\u4ee5\u53ca\u80bf\u7624\u542b\u91cf\u662f\u5426\u8fbe\u6807\uff0c\u82e5\u4e0d\u7b26\u5408\u5219\u53ef\u8003\u8651\u91cd\u65b0\u53d6\u6837\uff0c\u8fbe\u6807\u540e\u518d\u9001\u68c0\uff0c\u82e5\u7b26\u5408\u6807\u51c6\u5219\u7ed3\u679c\u57fa\u672c\u53ef\u4fe1\uff0c\u518d\u884c\u68c0\u6d4b\u6539\u53d8\u53ef\u80fd\u6027\u4e0d\u5927\uff1b<\/p>\n
4. \u5ba1\u89c6\u68c0\u6d4b\u6280\u672f\uff0c\u82e5\u764c\u79cd\u3001\u6837\u672c\u5747\u65e0\u95ee\u9898\uff0c\u4f7f\u7528\u7684\u662fPCR\u6280\u672f\u68c0\u6d4b\uff0c\u7ecf\u6d4e\u5141\u8bb8\u4e14\u5269\u4f59\u6837\u672c\u8db3\u591f\u7684\u60c5\u51b5\u4e0b\u53ef\u8003\u8651\u518d\u884cNGS\u6280\u672f\u68c0\u6d4b\uff0c\u4ee5\u514d\u9057\u6f0f\u5c0f\u6982\u7387\u7684\u7f55\u89c1\u7a81\u53d8\u4f4d\u70b9\u53ca\u7c7b\u578b\u3002<\/p>\n
5. \u82e5\u764c\u79cd\u3001\u6837\u672c\uff0c\u8fd8\u6709\u68c0\u6d4b\u6280\u672f\u5747\u65e0\u95ee\u9898\uff0c\u90a3\u4e48\u57fa\u56e0\u68c0\u6d4b\u7ed3\u679c\u95ee\u9898\u4e0d\u5927\uff0c\u57fa\u672c\u4e0d\u4f1a\u6709\u9519\u6f0f\uff0c\u77ed\u671f\u5185\u4e0d\u9700\u8981\u4e8c\u6b21\u68c0\u6d4b\uff0c\u6539\u53d8\u53ef\u80fd\u6027\u4e5f\u4e0d\u9ad8\uff0c\u5b9e\u5728\u60f3\u640f\u4e00\u640f\u5efa\u8bae\u5728\u63a5\u53d7\u6b63\u89c4\u6cbb\u7597\u51fa\u73b0\u8fdb\u5c55\uff0c\u60c5\u51b5\u6709\u53d8\u5316\u4e4b\u540e\u624d\u8003\u8651\u3002<\/p>\n
\u672c\u6587\u7ae0\u8f6c\u8f7d\u4e8e\uff1a \u4e0e\u7231\u5171\u821e\u8ba2\u9605\u53f7<\/p>\n
\u539f\u6587\u94fe\u63a5\uff1ahttps:\/\/mp.weixin.qq.com\/s\/w6yaj-CImt27KZFIDDHHQw<\/p>\n<\/div><\/div><\/div><\/div><\/div><\/p>","protected":false},"excerpt":{"rendered":"
Patients who have just been diagnosed with non-small cell lung cancer have raised a common question: \"What should I do if the first genetic test shows no mutation? Do I need to retest?\" Regarding this issue, I would like to propose the following ideas from the perspective of genetic testing for your reference.<\/p>","protected":false},"author":1,"featured_media":3606,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"content-type":"","om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[202],"tags":[],"class_list":["post-1323","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-202"],"acf":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/posts\/1323","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/comments?post=1323"}],"version-history":[{"count":6,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/posts\/1323\/revisions"}],"predecessor-version":[{"id":3618,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/posts\/1323\/revisions\/3618"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/media\/3606"}],"wp:attachment":[{"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/media?parent=1323"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/categories?post=1323"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/tags?post=1323"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}
\u6700\u540e\u5e2e\u5404\u4f4d\u68b3\u7406\u4e00\u4e0b\u82e5\u57fa\u56e0\u68c0\u6d4b\u62a5\u544a\u9634\u6027\uff0c\u4ece\u57fa\u56e0\u68c0\u6d4b\u89d2\u5ea6\uff0c\u8fd8\u53ef\u4ee5\u505a\u54ea\u4e9b\u5c1d\u8bd5\uff1a<\/p>\n
1. \u56de\u5f52\u75c5\u7406\u62a5\u544a\uff0c\u786e\u8ba4\u81ea\u8eab\u764c\u79cd\uff0c\u4e86\u89e3\u81ea\u8eab\u764c\u79cd\u662f\u5426\u6709\u5bf9\u5e94\u9776\u5411\u836f\u4ee5\u53ca\u9776\u5411\u57fa\u56e0\u7a81\u53d8\u53ef\u80fd\u6027\u5927\u5c0f\uff0c\u82e5\u65e0\u76f8\u5173\u9776\u5411\u836f\uff0c\u5219\u518d\u6b21\u57fa\u56e0\u68c0\u6d4b\u610f\u4e49\u4e0d\u5927\uff1b<\/p>\n
2. \u56de\u987e\u68c0\u6d4b\u4f7f\u7528\u7684\u6837\u672c\u7c7b\u578b\uff0c\u82e5\u4ec5\u4f7f\u7528\u8840\u6db2\u6837\u672c\u4e14\u672a\u68c0\u6d4b\u5230\u4efb\u4f55\u7a81\u53d8\uff0c\u5219\u6709\u6761\u4ef6\u7684\u60c5\u51b5\u4e0b\u53ef\u8003\u8651\u5c3d\u91cf\u83b7\u53d6\u7ec4\u7ec7\u6837\u672c\uff0c\u5728\u786e\u5b9a\u764c\u79cd\u7c7b\u578b\u9002\u5408\u540e\u7528\u7ec4\u7ec7\u6837\u672c\u518d\u884c\u68c0\u6d4b\uff1b<\/p>\n
3. \u56de\u987e\u68c0\u6d4b\u4f7f\u7528\u7684\u6837\u672c\u7c7b\u578b\uff0c\u82e5\u4f7f\u7528\u7684\u4e3a\u65b0\u9c9c\u6807\u672c\u6216\u6837\u672c\u91cf\u4e0d\u8db3\u7684\u77f3\u8721\u5207\u7247\uff0c\u5219\u4ecd\u662f\u56de\u5f52\u75c5\u7406\u62a5\u544a\uff0c\u5148\u786e\u8ba4\u6b64\u6b21\u53d6\u6837\u662f\u5426\u53d6\u5230\u80bf\u7624\u4ee5\u53ca\u80bf\u7624\u542b\u91cf\u662f\u5426\u8fbe\u6807\uff0c\u82e5\u4e0d\u7b26\u5408\u5219\u53ef\u8003\u8651\u91cd\u65b0\u53d6\u6837\uff0c\u8fbe\u6807\u540e\u518d\u9001\u68c0\uff0c\u82e5\u7b26\u5408\u6807\u51c6\u5219\u7ed3\u679c\u57fa\u672c\u53ef\u4fe1\uff0c\u518d\u884c\u68c0\u6d4b\u6539\u53d8\u53ef\u80fd\u6027\u4e0d\u5927\uff1b<\/p>\n
4. \u5ba1\u89c6\u68c0\u6d4b\u6280\u672f\uff0c\u82e5\u764c\u79cd\u3001\u6837\u672c\u5747\u65e0\u95ee\u9898\uff0c\u4f7f\u7528\u7684\u662fPCR\u6280\u672f\u68c0\u6d4b\uff0c\u7ecf\u6d4e\u5141\u8bb8\u4e14\u5269\u4f59\u6837\u672c\u8db3\u591f\u7684\u60c5\u51b5\u4e0b\u53ef\u8003\u8651\u518d\u884cNGS\u6280\u672f\u68c0\u6d4b\uff0c\u4ee5\u514d\u9057\u6f0f\u5c0f\u6982\u7387\u7684\u7f55\u89c1\u7a81\u53d8\u4f4d\u70b9\u53ca\u7c7b\u578b\u3002<\/p>\n
5. \u82e5\u764c\u79cd\u3001\u6837\u672c\uff0c\u8fd8\u6709\u68c0\u6d4b\u6280\u672f\u5747\u65e0\u95ee\u9898\uff0c\u90a3\u4e48\u57fa\u56e0\u68c0\u6d4b\u7ed3\u679c\u95ee\u9898\u4e0d\u5927\uff0c\u57fa\u672c\u4e0d\u4f1a\u6709\u9519\u6f0f\uff0c\u77ed\u671f\u5185\u4e0d\u9700\u8981\u4e8c\u6b21\u68c0\u6d4b\uff0c\u6539\u53d8\u53ef\u80fd\u6027\u4e5f\u4e0d\u9ad8\uff0c\u5b9e\u5728\u60f3\u640f\u4e00\u640f\u5efa\u8bae\u5728\u63a5\u53d7\u6b63\u89c4\u6cbb\u7597\u51fa\u73b0\u8fdb\u5c55\uff0c\u60c5\u51b5\u6709\u53d8\u5316\u4e4b\u540e\u624d\u8003\u8651\u3002<\/p>\n
\u672c\u6587\u7ae0\u8f6c\u8f7d\u4e8e\uff1a \u4e0e\u7231\u5171\u821e\u8ba2\u9605\u53f7<\/p>\n
\u539f\u6587\u94fe\u63a5\uff1ahttps:\/\/mp.weixin.qq.com\/s\/w6yaj-CImt27KZFIDDHHQw<\/p>\n<\/div><\/div><\/div><\/div><\/div><\/p>","protected":false},"excerpt":{"rendered":"
Patients who have just been diagnosed with non-small cell lung cancer have raised a common question: \"What should I do if the first genetic test shows no mutation? Do I need to retest?\" Regarding this issue, I would like to propose the following ideas from the perspective of genetic testing for your reference.<\/p>","protected":false},"author":1,"featured_media":3606,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"content-type":"","om_disable_all_campaigns":false,"_monsterinsights_skip_tracking":false,"_monsterinsights_sitenote_active":false,"_monsterinsights_sitenote_note":"","_monsterinsights_sitenote_category":0,"footnotes":""},"categories":[202],"tags":[],"class_list":["post-1323","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-202"],"acf":[],"aioseo_notices":[],"_links":{"self":[{"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/posts\/1323","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/comments?post=1323"}],"version-history":[{"count":6,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/posts\/1323\/revisions"}],"predecessor-version":[{"id":3618,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/posts\/1323\/revisions\/3618"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/media\/3606"}],"wp:attachment":[{"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/media?parent=1323"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/categories?post=1323"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.funcbio.cn\/en\/wp-json\/wp\/v2\/tags?post=1323"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}